The Role of miR-378a in Metabolism, Angiogenesis, and Muscle Biology

Krist, Bart; Florczyk, Urszula; Pietraszek-Gremplewicz, Katarzyna; Józkowicz, Alicja; Dulak, Józef

doi:10.1155/2015/281756

Cited by 119 publications

(101 citation statements)

References 133 publications

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“…Growing evidence has demonstrated that miR-378a-3p is involved in a wide range of biological functions and processes such as cell growth, cell cycle, migration, differentiation, metabolism and angiogenesis [12,15]. A recent study shows that miR-378a-3p, a negative regulator of TGFβ1, contributes to the suppression of cardiac fibrosis [16].…”

Section: Introductionmentioning

confidence: 99%

Activation of Hepatic Stellate Cells is Inhibited by microRNA-378a-3p via Wnt10a

Fan

Chen

et al. 2016

Cell Physiol Biochem

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Background/Aims: Wnt/β-catenin pathway is involved in liver fibrosis and microRNAs (miRNAs) are considered as key regulators of the activation of hepatic stellate cells (HSCs). A recent study showed the protective role of miR-378a-3p against cardiac fibrosis. However, whether miR-378a-3p suppresses Wnt/β-catenin pathway in liver fibrosis is largely unknown. Methods: miR-378a-3p expression was detected in carbon tetrachloride-induced liver fibrosis and activated HSCs. Effects of miR-378a-3p overexpression on HSC activation and Wnt/β-catenin pathway were analyzed. Bioinformatic analysis was employed to identify the potential targets of miR-378a-3p. Serum miR-378a-3p expression was analyzed in patients with cirrhosis. Results: Reduced miR-378a-3p expression was observed in the fibrotic liver tissues and activated HSCs. Up-regulation of miR-378a-3p inhibited HSC activation including cell proliferation, α-smooth muscle actin (α-SMA) and collagen expression. Moreover, miR-378a-3p overexpression resulted in Wnt/β-catenin pathway inactivation. Luciferase reporter assays demonstrated that Wnt10a, a member of Wnt/β-catenin pathway, was confirmed to be a target of miR-378a-3p. By contrast, miR-378a-3p inhibitor contributed to HSC activation, with an increase in cell proliferation, α-SMA and collagen expression. But all these effects were blocked down by silencing of Wnt10a. Notably, sera from patients with cirrhosis contained lower levels of miR-378a-3p than sera from healthy controls. Receiver operating characteristic curve analysis suggested that serum miR-378a-3p differentiated liver cirrhosis patients from healthy controls, with an area under the curve of ROC curve of 0.916. Conclusion: miR-378a-3p suppresses HSC activation, at least in part, via targeting of Wnt10a, supporting its potential utility as a novel therapeutic target for liver fibrosis.

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Section: Introductionmentioning

confidence: 99%

Activation of Hepatic Stellate Cells is Inhibited by microRNA-378a-3p via Wnt10a

Fan

Chen

et al. 2016

Cell Physiol Biochem

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“…In addition to myomiRs, previous studies have revealed the roles of other abundantly expressed miRNAs in skeletal myogenesis. For example, miR-378-3p could increase the expression of MyoD by repressing MyoR in myoblast cells [39]; miR-424 promoted C2C12 myoblast differentiation via direct targeting of the 3´UTR of Cdc25A [40]. Ge et al [41] have reported that miR-125b-5p negatively modulated muscle regeneration in mice, while IGF-2 is a direct target of miR-125b-5p.…”

Section: Discussionmentioning

confidence: 99%

Identification and Expression Profiling of miRNAome in Goat longissimus dorsi Muscle from Prenatal Stages to a Neonatal Stage

et al. 2016

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Skeletal muscle development is a complex biological process regulated by numerous genes and non-coding RNAs, such as microRNAs (miRNAs). In the current study, we made use of the deep sequencing data from Jianzhou Da’er goat longissimus dorsi sampled on days 45, 60, and 105 of gestation, as well as day three after birth to identify miRNAs that regulate goat skeletal myogenesis, and examine their temporal expression profiles. A total of 410 known goat miRNAs, 752 miRNA homologs and 88 novel miRNAs were identified across four stages. Besides three myomiRs, the abundance of 17 miRNAs, including chi-miR-424, chi-miR-542-3p and chi-miR-136-5p was more than 10,000 reads per million mapped reads (RPM), on average. Furthermore, 50 miRNAs with more than 100 RPM clustered at the imprinted DLK1–DIO3 locus on chromosome 21 and showed similar expression patterns, indicating that these miRNAs played important roles in skeletal myogenesis of goats. Based on pairwise comparisons, 221 differentially expressed (DE), known miRNAs were identified across four stages. GO and KEGG analyses of the genes targeted by the DE miRNAs revealed the significantly enriched processes and pathways to be consistent with temporal changes of skeletal muscle development across all sampled stages. However, follow-up experimental studies were required to explore functions of these miRNAs and targets underlying skeletal myogenesis.

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“…Studies of muscle development often explore gene expression under different conditions (Krist et al, 2015; Wang, Xiao & Wang, 2016; Zhang et al, 2016a; Zhang et al, 2015). The expressions of most genes display variable expression levels in prenatal and postnatal periods, and reference genes that are frequently used for other experiments are not suitable for studies of skeletal muscle development.…”

Section: Introductionmentioning

confidence: 99%

Identifying suitable reference genes for gene expression analysis in developing skeletal muscle in pigs

Niu

Yang

Zhang

et al. 2016

PeerJ

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The selection of suitable reference genes is crucial to accurately evaluate and normalize the relative expression level of target genes for gene function analysis. However, commonly used reference genes have variable expression levels in developing skeletal muscle. There are few reports that systematically evaluate the expression stability of reference genes across prenatal and postnatal developing skeletal muscle in mammals. Here, we used quantitative PCR to examine the expression levels of 15 candidate reference genes (ACTB, GAPDH, RNF7, RHOA, RPS18, RPL32, PPIA, H3F3, API5, B2M, AP1S1, DRAP1, TBP, WSB, and VAPB) in porcine skeletal muscle at 26 different developmental stages (15 prenatal and 11 postnatal periods). We evaluated gene expression stability using the computer algorithms geNorm, NormFinder, and BestKeeper. Our results indicated that GAPDH and ACTB had the greatest variability among the candidate genes across prenatal and postnatal stages of skeletal muscle development. RPS18, API5, and VAPB had stable expression levels in prenatal stages, whereas API5, RPS18, RPL32, and H3F3 had stable expression levels in postnatal stages. API5 and H3F3 expression levels had the greatest stability in all tested prenatal and postnatal stages, and were the most appropriate reference genes for gene expression normalization in developing skeletal muscle. Our data provide valuable information for gene expression analysis during different stages of skeletal muscle development in mammals. This information can provide a valuable guide for the analysis of human diseases.

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The Role of miR-378a in Metabolism, Angiogenesis, and Muscle Biology

Cited by 119 publications

References 133 publications

Activation of Hepatic Stellate Cells is Inhibited by microRNA-378a-3p via Wnt10a

Activation of Hepatic Stellate Cells is Inhibited by microRNA-378a-3p via Wnt10a

Identification and Expression Profiling of miRNAome in Goat longissimus dorsi Muscle from Prenatal Stages to a Neonatal Stage

Identifying suitable reference genes for gene expression analysis in developing skeletal muscle in pigs

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