The role of miRNA in somatic embryogenesis

Siddiqui, Zahid Hameed; Abbas, Zahid Khorshid; Ansari, Mohammad Wahid; Khan, Md. Raisuddin

doi:10.1016/j.ygeno.2018.11.022

Cited by 54 publications

(22 citation statements)

References 76 publications

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“…The mechanism of DNA methylation-mediated gene regulation in SE induction might also involve the miRNAs that have an essential regulatory function in SE induction [ 155 ]. Relevantly, the miRNAs that operate at the chromatin level to direct the methylation of specific coding sequences have been identified, including a particular class of longer miRNAs [ 156 ].…”

Section: Discussionmentioning

confidence: 99%

Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis

Grzybkowska

Nowak

Gaj

2020

IJMS

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The auxin-induced embryogenic reprogramming of plant somatic cells is associated with extensive modulation of the gene expression in which epigenetic modifications, including DNA methylation, seem to play a crucial role. However, the function of DNA methylation, including the role of auxin in epigenetic regulation of the SE-controlling genes, remains poorly understood. Hence, in the present study, we analysed the expression and methylation of the TF genes that play a critical regulatory role during SE induction (LEC1, LEC2, BBM, WUS and AGL15) in auxin-treated explants of Arabidopsis. The results showed that auxin treatment substantially affected both the expression and methylation patterns of the SE-involved TF genes in a concentration-dependent manner. The auxin treatment differentially modulated the methylation of the promoter (P) and gene body (GB) sequences of the SE-involved genes. Relevantly, the SE-effective auxin treatment (5.0 µM of 2,4-D) was associated with the stable hypermethylation of the P regions of the SE-involved genes and a significantly higher methylation of the P than the GB fragments was a characteristic feature of the embryogenic culture. The presence of auxin-responsive (AuxRE) motifs in the hypermethylated P regions suggests that auxin might substantially contribute to the DNA methylation-mediated control of the SE-involved genes.

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Section: Discussionmentioning

confidence: 99%

Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis

Grzybkowska

Nowak

Gaj

2020

IJMS

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“…In particular, several studies reporting microRNA expression patterns, both from model plant and some cultivated crop tissues, during different bacterial infections showed that bacterial elements trigger the up- or downregulation of specific microRNAs, which suppress or induce key negative or positive regulators of the host defense (reviewed by [ 78 ]). In addition, key microRNAs involved in somatic embryogenesis [ 79 ] suggest that increasing our understanding about the role of these molecules could also contribute to improved gene transfer protocols based on SE. In peach, several microRNAs involved in response to different stress conditions have been identified [ 25 , 80 , 81 , 82 , 83 , 84 ]; nevertheless, microRNA expression profiles from Agrobacterium -mediated transformed or infected tissues has not been built to date.…”

Section: Possible Solutions To the Bottleneckmentioning

confidence: 99%

Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward

Ricci

Sabbadini

Prieto

et al. 2020

Plants

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Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established. Many strategies have been tested in order to obtain an efficient peach transformation system. Despite the amount of time and the efforts invested, the lack of success has significantly limited the utility of peach as a model genetic system for trees, despite its relatively short generation time; small, high-quality genome; and well-studied genetic resources. Additionally, the absence of efficient genetic transformation protocols precludes the application of many biotechnological tools in peach breeding programs. In this review, we provide an overview of research on regeneration and genetic transformation in this species and summarize novel strategies and procedures aimed at producing transgenic peaches. Promising future approaches to develop a robust peach transformation system are discussed, focusing on the main bottlenecks to success including the low efficiency of A. tumefaciens-mediated transformation, the low level of correspondence between cells competent for transformation and those that have regenerative competence, and the high rate of chimerism in the few shoots that are produced following transformation.

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“…The occurrence of somatic embryogenesis is associated with the induction of differentiated explant tissue cells that acquire the embryogenic characteristic, followed by the expression of the somatic embryo [19][20][21][22]. Thus, this process consists in the termination of the gene expression model present in the explant differentiated tissue cells that will be replaced by the expression of embryogenic genes [23,24]. But embryogenic program does not occur in all cells at the same time, only in some of them [14,17,25,26].…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Observations on Somatic Embryogenesis inCoffea arabicaL.

Almeida¹

2020

Coffee - Production and Research

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Somatic embryogenesis contributes to coffee breeding programs. This is a process of asexual reproduction which is based on the concept of cellular totipotency. Each haploid or somatic cell of the plant tissue has the genetic information necessary to generate a complete and functional plant. The somatic embryogenesis can occur either indirectly or directly. Coffea arabica genotypes may respond to direct, indirect, or both. In this species, the indirect somatic embryogenesis is composed of two phases, the callogenesis and the embryogenesis, while the direct pathway occurs in a single phase, without the callogenesis. In Coffea, in general, the indirect pathway is induced by the auxin and cytokinin, and the direct pathway with cytokinin only. C. arabica genotypes usually respond easily to the indirect route with high production of somatic embryos. But these are inefficient by the direct route because they present low production of embryos and the process occurs for a long time. In this review, emphasis will be given to different events that are part of the somatic embryogenesis of C. arabica occurring indirectly and in the direct pathway as well as factors that may affect its control.

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The role of miRNA in somatic embryogenesis

Cited by 54 publications

References 76 publications

Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis

Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis

Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward

Observations on Somatic Embryogenesis inCoffea arabicaL.

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