The role of mitochondria in sulfolipid biosynthesis by Euglena chloroplasts

Saidha, Tekchand; Schiff, Jerome A.

doi:10.1016/0005-2760(89)90110-0

Cited by 15 publications

(14 citation statements)

References 26 publications

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“…1. Labelled sulpholipid is also found in all cells except those of W,, (Saidha & Schiff, 1989) which lack plastids completely (Osafune & Schiff, 1983).…”

Section: Resultsmentioning

confidence: 99%

“…bacillaris and mutants W, (lanes 5 and 6) and W,, (lanes 8 and 9) incubated with carrier-free 35SOi-in the light for the indicated times. Lanes 1, 4 and 7 received [35S]sulpholipid standard prepared from light-grown wild-type Euglena as described previously (Saidha & Schiff, 1989). Spots A, B and C represent the unknown lipids and SL indicates sulpholipid.…”

Section: Resultsmentioning

confidence: 99%

“…W1,, therefore, does not make the sulpholipid (Saidha & Schiff, 1989). Although traces of other radioactive spots could occasionally be seen on these chromatograms, their nature only became apparent in a study of resting (non-dividing) cells of Euglena incubated with 35SOf on a medium containing mannitol (not utilized as a carbon source for growth by EugZena), MgC1, and KH,PO,.…”

Section: Introductionmentioning

confidence: 97%

“…bacillaris grown in darkness or light on a complete medium with 35SOi-show a single major labelled spot on thin-layer silica gel chromatography corresponding to the thylakoid sulpholipid (diacylsulphoquinovos ylgl ycerol) ; much higher labelling is observed in the light than in the dark (Saidha & Schiff, 1989). Mutant W,BUL (W,) retains only a proplastid remnant but also shows light-stimulated accumulation of the sulpholipid under dividing conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Mutant W,BUL (W,) retains only a proplastid remnant but also shows light-stimulated accumulation of the sulpholipid under dividing conditions. W, yields a chromatographic pattern much like the wild-type with a single major spot corresponding to the sulpholipid (Saidha & Schiff, 1989). Dividing cells of mutant W,,BSmL (W,,) lack plastids completely (Osafune & Schiff, 1983) and show no major radioactive spot under the same conditions in either light or *Author for correspondence.…”

Section: Introductionmentioning

confidence: 99%

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Taurine conjugates in the lipid fraction of Euglena cells and their mitochondria

Saidha¹,

Stern²,

Schiff³

1993

Journal of General Microbiology

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Dark-grown resting (non-dividing) cells of EugZena gracilis var. baciZZaris and mutants W,BUL (with a proplastid remnant) and W,,,BSmL (lacking plastids) incubated with 3sSOi-form a series of labelled lipids which are low or absent in dividing cells. These lipids all release labelled taurine on mild acid-hydrolysis. Treatment of the labelled lipids with 2,4-dinitrofluorobenzene (DNFB) followed by acid hydrolysis does not yield labelled dinitrophenyltaurine (DNP-taurine), but treatment with DNFB after hydrolysis readily forms labelled DNP-taurine, indicating that taurine is linked to the lipids by at least the amino group. Illumination increases the labelling of these taurolipids in plastid-containing cells (wild-type and W,BUL) but has little effect in cells lacking plastids (W,,,BSmL); labelling is highest in W,, cells irrespective of illumination. This indicates that the presence of a plastid may exert a negative control on taurolipid formation which is relieved by light. The same series of labelled lipids is found in isolated purified mitochondria from mutant W,,, indicating that this organelle is a site for taurolipid deposition, The formation of taurolipids under non-dividing conditions may be a response to nutritional stress and these negatively charged constituents (as well as the thylakoid sulpholipid) may serve to protect membranes by repelling deleterious negatively charged oxygen species.

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“…1. Labelled sulpholipid is also found in all cells except those of W,, (Saidha & Schiff, 1989) which lack plastids completely (Osafune & Schiff, 1983).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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