The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria

Morales, Grace; Abelson, Benjamin; Reasoner, Seth; Miller, Jordan A.; Earl, Ashlee M.; Hadjifrangiskou, Maria; Schmitz, Jonathan E.

doi:10.1128/spectrum.04710-22

Cited by 9 publications

(4 citation statements)

References 92 publications

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“…However, the urinary asymptomatic carriage of these bacteria has been also established, with a mucosa remaining inert despite large numbers of bacteria in urine 17 . As the host's immunity status is an important element involved in the equilibrium between urinary tract colonization versus infection, the genetic variations of Escherichia could also explain the asymptomatic cases in our population 18 . In contrast, a significant increase of Proteus abundance was observed in patients with stagnated/worsened wounds.…”

Section: Discussionmentioning

confidence: 84%

“… 17 As the host's immunity status is an important element involved in the equilibrium between urinary tract colonization versus infection, the genetic variations of Escherichia could also explain the asymptomatic cases in our population. 18 In contrast, a significant increase of Proteus abundance was observed in patients with stagnated/worsened wounds. This evolution could be linked to the persistence of Proteus in the PU at D28, as previously documented, 10 reflecting the continuum between urinary microbiota and chronic wounds colonization.…”

Section: Discussionmentioning

confidence: 96%

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Evolution of the urinary microbiota in spinal cord injury patients with decubitus ulcer: A snapshot study

Morsli,

Salipante,

Gelis

et al. 2024

International Wound Journal

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Current microbiome investigations of patients with pressure ulcers (PU) are mainly based on wound swabs and/or biopsy sequencing, leaving the colonization scenario unclear. Urinary microbiota has been never studied. As a part of the prospective ESCAFLOR study, we studied urinary microbiota of spinal cord injury (SCI) patients with PU without any urinary tract infection at the inclusion, collected at two times (at admission [D0] and after 28 days [D28]) during the patient's care, investigated by 16S rDNA metagenomics next generation sequencing. Subgroup analyses were carried out between patients with wounds showing improved evolution versus stagnated/worsened wounds at D28. Analysis was done using EPISEQ® 16S and R software. Among the 12 studied patients, the urinary microbiota of patients with improved wound evolution at D28 (n = 6) presented a significant decrease of microbial diversity. This modification was associated with the presence of Proteobacteria phylum and an increase of Escherichia‐Shigella (p = 0.005), as well as the presence of probiotic anaerobic bacteria Lactobacillus and Bifidobacterium. In contrast, Proteus abundance was significantly increased in urine of patients with stagnated/worsened wound evolution (n = 6) (p = 0.003). This study proposes urinary microbiota as a complementary factor indirectly associated with the wound evolution and patient cure. It opens new perspectives for further investigations based on multiple body microbiome comparison to describe the complete scenario of the transmission dynamics of wound‐colonizing microorganisms.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 96%

Evolution of the urinary microbiota in spinal cord injury patients with decubitus ulcer: A snapshot study

Morsli,

Salipante,

Gelis

et al. 2024

International Wound Journal

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“…Identification of mobile genetic elements in diverse species provides clues to how certain virulence or antimicrobial resistance were acquired (Vale et al, 2022;Morales et al, 2023). Based on the gene analysis, we have identified presence of mefA and msrD in an intact prophage which likely provide evidence where strain SS/UPM/MY/F001 had acquired this resistance through prophage transduction.…”

Section: Discussionmentioning

confidence: 90%

Whole genome sequencing of Streptococcus suis revealed potential drug resistance and zoonotic transmission in companion cat

C.Y.

2024

Trop Biomed

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Streptococcus suis is a bacterium of clinical importance in diverse animal hosts including companion animals and humans. Companion animals are closely associated in the living environment of humans and are potential reservoirs for zoonotic pathogens. Given the zoonotic potential of S. suis, it is crucial to determine whether this bacterium is present among the companion animal population. This study aimed to detect Streptococcus suis in companion animals namely cats and dogs of the central west coast of Peninsular Malaysia and further characterize the positive isolates via molecular and genomic approach. The detection of S. suis was done via bacterial isolation and polymerase chain reaction assay of gdh and recN gene from oral swabs. Characterization was done by multiplex PCR serotyping, as well as muti-locus sequence typing, AMR gene prediction, MGE identification and phylogenomic analysis on whole genome sequence acquired from Illumina and Oxford Nanopore sequencing. Among the 115 samples, PCR assay detected 2/59 of the cats were positive for S. suis serotype 8 while all screened dog samples were negative. This study further described the first complete whole genome of S. suis strain SS/UPM/MY/F001 isolated from the oral cavity of a companion cat. Genomic analysis revealed a novel strain of S. suis having a unique MLST profile and antimicrobial resistance genes of mefA, msrD, patA, patB and vanY. Mobile genetic elements were described, and pathogenic determinants matched to human and swine strains were identified. Phylogenetic tree analysis on the core genome alignment revealed strain SS/UPM/MY/F001 was distinct from other S. suis strains. This study provided insight into the detection and genomic features of the S. suis isolate of a companion cat and highlighted its potential for antimicrobial resistance and pathogenicity.

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“…A limitation of the current investigation is that, although we included multiple uropathogens including two prototypical strains of UPEC (CFT073 and UTI89), ABU 83972 was the sole ABU strain utilized in the competition assays. Even though the model ABU strain E. coli 83972 is an effective competitor of uropathogens, additional ABU strains have been identified and characterized ( 52 , 53 ). Alternative ABU E. coli strains such as ABU 61, ABU 106, and ABU 123 have been found to be more competitive than ABU 83972, with low in vivo pathogenicity in a murine sepsis model and antibiotic sensitivity ( 52 ).…”

Section: Discussionmentioning

confidence: 99%

Competitive fitness of asymptomatic bacteriuria E. coli strain 83972 against uropathogens in human urine

George,

Kalairaj,

Zimmern

et al. 2024

Infect Immun

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Urinary tract infection (UTI) is one of the most common bacterial infections worldwide. The main causative agent of UTI is uropathogenic Escherichia coli (UPEC). There is an immediate need for novel prophylactic and treatment strategies against UTI because of the increasing incidence of antimicrobial resistance among uropathogens. ABU 83972, an asymptomatic bacteriuria-causing E. coli strain , prevents UTI by suppressing the colonization of UPEC. However, the nature of competition and growth repression of UPEC by ABU 83972 is unclear and is the subject of our investigation. Here, we characterized the growth kinetics of ABU 83972 and uropathogens in human urine and laboratory media. Next, we performed a series of competitive co-culture experiments where ABU 83972 and uropathogens were inoculated at a 1:1 ratio in human urine and in various media, and their relative abundance was determined. In human urine, ABU 83972 outcompeted UPEC and additional uropathogens, reaching up to 90% of the total population after 24 hours of incubation. In contrast, UPEC outcompeted ABU 83972 in LB and M9 minimal media and exhibited superior colonization than ABU 83972 in the mouse urinary bladder. Since engineered living materials (ELMs) can be used to retain an organism of interest in a particular location, we developed ABU 83972-containing ELMs that effectively outcompeted UPEC in human urine. In summary, our work establishes that ABU 83972 outcompetes UPEC in a milieu- and cell-density-dependent manner, highlighting the importance of the metabolites and nutrients found in the human urine as determinants of the competitive fitness of ABU 83972.

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The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria

Cited by 9 publications

References 92 publications

Evolution of the urinary microbiota in spinal cord injury patients with decubitus ulcer: A snapshot study

Evolution of the urinary microbiota in spinal cord injury patients with decubitus ulcer: A snapshot study

Whole genome sequencing of Streptococcus suis revealed potential drug resistance and zoonotic transmission in companion cat

Competitive fitness of asymptomatic bacteriuria E. coli strain 83972 against uropathogens in human urine

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