The role of MRI in applying the 3Rs to non-human primate neuroscience

Prescott, Mark J.; Poirier, Colline

doi:10.1016/j.neuroimage.2020.117521

Cited by 9 publications

(6 citation statements)

References 54 publications

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“…This method supplements our proposed modeling technique and is a welcome alternative to live MRI, which requires specialized equipment and facilities often unavailable to researchers. In sum, we propose our method as an additional way of applying the principles of replacement, reduction, and refinement (3Rs) to injections in NHPs [ 44 , 45 ]. We recommend NHP CED infusions be modeled in advance of surgery with our proposed method to reduce the number of animals, replace an excess of pilot procedures with artificial simulations, and refine the overall technique to reduce harm.…”

Section: Discussionmentioning

confidence: 99%

Improving the Efficacy and Accessibility of Intracranial Viral Vector Delivery in Non-Human Primates

Griggs

Garcia

et al. 2022

Pharmaceutics

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Non-human primates (NHPs) are precious resources for cutting-edge neuroscientific research, including large-scale viral vector-based experimentation such as optogenetics. We propose to improve surgical outcomes by enhancing the surgical preparation practices of convection-enhanced delivery (CED), which is an efficient viral vector infusion technique for large brains such as NHPs’. Here, we present both real-time and next-day MRI data of CED in the brains of ten NHPs, and we present a quantitative, inexpensive, and practical bench-side model of the in vivo CED data. Our bench-side model is composed of food coloring infused into a transparent agar phantom, and the spread of infusion is optically monitored over time. Our proposed method approximates CED infusions into the cortex, thalamus, medial temporal lobe, and caudate nucleus of NHPs, confirmed by MRI data acquired with either gadolinium-based or manganese-based contrast agents co-infused with optogenetic viral vectors. These methods and data serve to guide researchers and surgical team members in key surgical preparations for intracranial viral delivery using CED in NHPs, and thus improve expression targeting and efficacy and, as a result, reduce surgical risks.

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Section: Discussionmentioning

confidence: 99%

Improving the Efficacy and Accessibility of Intracranial Viral Vector Delivery in Non-Human Primates

Griggs

Garcia

et al. 2022

Pharmaceutics

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“…Quantitative MRI data from a given human neurology case is often compared to other patients of the same type and to normative data, informing patient-specific diagnosis, treatment and decision-making, whenever the patient's results are well outside of normative distributions. While this approach is available for human patients, outside of MRI information from specific disease models (e.g., Parkinson's disease ( Luan et al., 2008 ), stroke ( Li et al., 2018 )), MRI monitoring more broadly for nonhuman primates on neuroscientific procedures is largely missing, and a resource and framework for sharing these type of data are needed, as noted by ( Bridge et al., 2019 ; Prescott and Poirier, 2020 ; Basso et al., 2021 ). However, if established such an approach and resource could be of substantial benefit for animal welfare and in support of biomedical and scientific advance.…”

Section: Discussionmentioning

confidence: 99%

“…While the MRI-compatible implants used in these cases can be stable for years in the vast majority of the cases, customised, MRI-compatible hydroxyapatite coated implants are being developed and tested ( Ortiz-Rios et al., 2018 ). Developments such as these could be assessed and compared to other implant options using MRI monitoring ( Prescott and Poirier, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Also, given the rarity of such cases and how few any one institution might have, it is important to establish a resource to which the global scientific community can contribute, such as within the PRIMatE Data Exchange, PRIME-DE ( Milham et al., 2018 , 2020 ). This will help to advance the 3Rs and animal welfare ( Prescott and Poirier, 2020 ) and support delivery on scientific and biomedical advances (see Editorial Introduction this issue; Basso et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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MRI monitoring of macaque monkeys in neuroscience: Case studies, resource and normative data comparisons

et al. 2021

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Information from Magnetic Resonance Imaging (MRI) is useful for diagnosis and treatment management of human neurological patients. MRI monitoring might also prove useful for non-human animals involved in neuroscience research provided that MRI is available and feasible and that there are no MRI contra-indications precluding scanning. However, MRI monitoring is not established in macaques and a resource is urgently needed that could grow with scientific community contributions. Here we show the utility and potential benefits of MRI-based monitoring in a few diverse cases with macaque monkeys. We also establish a PRIMatE MRI Monitoring (PRIME-MRM) resource within the PRIMatE Data Exchange (PRIME-DE) and quantitatively compare the cases to normative information drawn from MRI data from typical macaques in PRIME-DE. In the cases, the monkeys presented with no or mild/moderate clinical signs, were well otherwise and MRI scanning did not present a significant increase in welfare impact. Therefore, they were identified as suitable candidates for clinical investigation, MRI-based monitoring and treatment. For each case, we show MRI quantification of internal controls in relation to treatment steps and comparisons with normative data in typical monkeys drawn from PRIME-DE. We found that MRI assists in precise and early diagnosis of cerebral events and can be useful for visualising, treating and quantifying treatment response. The scientific community could now grow the PRIME-MRM resource with other cases and larger samples to further assess and increase the evidence base on the benefits of MRI monitoring of primates, complementing the animals’ clinical monitoring and treatment regime.

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“…In the context of NHP imaging, functional localizers have distinctive added value compared to human imaging. The first most straightforward advantage is the increase in the number of subjects, when pooled across study sites, while at the same time allowing each study to comply with the reduction principle of the 3R’s (refinement, reduction, replacement) ( Prescott and Lidster, 2017 ; Prescott and Poirier, 2021 ; Tannenbaum and Bennett, 2015 ). This results in higher power in group analyses while at the same time offering a better description and understanding of inter-individual differences.…”

Section: Importance Of Functional Localizers In the Study Of Primate Brain Functionsmentioning

confidence: 99%

Common functional localizers to enhance NHP & cross-species neuroscience imaging research

et al. 2021

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Functional localizers are invaluable as they can help define regions of interest, provide cross-study comparisons, and most importantly, allow for the aggregation and meta-analyses of data across studies and laboratories. To achieve these goals within the non-human primate (NHP) imaging community, there is a pressing need for the use of standardized and validated localizers that can be readily implemented across different groups. The goal of this paper is to provide an overview of the value of localizer protocols to imaging research and we describe a number of commonly used or novel localizers within NHPs, and keys to implement them across studies. As has been shown with the aggregation of resting-state imaging data in the original PRIME-DE submissions, we believe that the field is ready to apply the same initiative for task-based functional localizers in NHP imaging. By coming together to collect large datasets across research group, implementing the same functional localizers, and sharing the localizers and data via PRIME-DE, it is now possible to fully test their robustness, selectivity and specificity. To do this, we reviewed a number of common localizers and we created a repository of well-established localizer that are easily accessible and implemented through the PRIME-RE platform.

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The role of MRI in applying the 3Rs to non-human primate neuroscience

Cited by 9 publications

References 54 publications

Improving the Efficacy and Accessibility of Intracranial Viral Vector Delivery in Non-Human Primates

Improving the Efficacy and Accessibility of Intracranial Viral Vector Delivery in Non-Human Primates

MRI monitoring of macaque monkeys in neuroscience: Case studies, resource and normative data comparisons

Common functional localizers to enhance NHP & cross-species neuroscience imaging research

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