The Role of Nanofat Grafting in Vulvar Lichen Sclerosus: A Preliminary Report

Tamburino, Serena; Lombardo, Gabriella; Tarico, Maria Stella; Perrotta, Rosario Emanuele

doi:10.5999/aps.2016.43.1.93

Cited by 37 publications

(32 citation statements)

References 7 publications

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“…With regard to symptoms, scales for measurement included continuous 14 and discrete 2 , 4 – 9 , 15 – 34 scales varying between 4 and 10 points. These scales focused on specific symptoms with itching, burning, and dyspareunia being the most common.…”

Section: Resultsmentioning

confidence: 99%

“…The most common signs measured were hyperkeratosis, erosion, atrophy, erythema, and purpuric lesions and/or itching-related excoriations. Both discrete 15 and continuous scales 4 – 9 , 18 , 20 , 21 , 23 , 27 , 29 , 32 , 36 – 42 were used to measure these signs. In addition to determining the specific sign, some studies added measurements of lesion size 28 , 29 , 38 , 41 , 43 as well as the duration of the lesion.…”

Section: Resultsmentioning

confidence: 99%

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Development of the Adult Vulvar Lichen Sclerosus Severity Scale—A Delphi Consensus Exercise for Item Generation

Sheinis

Selk

2018

J Low Genit Tract Dis

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Development of the Adult Vulvar Lichen Sclerosus Severity Scale—A Delphi Consensus Exercise for Item Generation

Sheinis

Selk

2018

J Low Genit Tract Dis

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“…The use of nanofat has been generalized and applied for cosmetic treatment in recent years [30, 31]. In the present study, NFSCs were isolated successfully and then identified by induction of multipotent differentiation and flow cytometry.…”

Section: Discussionmentioning

confidence: 91%

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

Liang

Lü

et al. 2018

Cell Physiol Biochem

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Background/Aims: The rejuvenation properties of nanofat grafting have been described in recent years. However, it is not clear whether the clinical efficacy of the procedure is attributable to stem cells or linked to other components of adipose tissue. In this study we isolated nanofat-derived stem cells (NFSCs) to observe their biological characteristics and evaluate the efficacy of precise intradermal injection of nanofat combined with platelet-rich fibrin (PRF) in patients undergoing facial rejuvenation treatment. Methods: Third-passage NFSCs were isolated and cultured using a mechanical emulsification method and their surface CD markers were analyzed by flow cytometry. The adipogenic and osteogenic nature and chondrogenic differentiation capacity of NFSCs were determined using Oil Red O staining, alizarin red staining, and Alcian blue staining, respectively. Paracrine function of NFSCs was evaluated by enzyme-linked immunosorbent assay (ELISA) at 1, 3, 7, 14, and 28 days after establishing the culture. Then, the effects of PRF on NFSC proliferation were assessed in vitro. Finally, we compared the outcome in 103 patients with facial skin aging who underwent both nanofat and intradermal PRF injection (treatment group) and 128 patients who underwent hyaluronic acid (HA) injection treatment (control group). Outcomes in the two groups were compared by assessing pictures taken at the same angle before and after treatment, postoperative recovery, incidence of local absorption and cysts, and skin quality before treatment, and at 1, 12, 24 months after treatment using the VISIA Skin Image Analyzer and a SOFT5.5 skin test instrument. Results: NFSCs expressed CD29, CD44, CD49d, CD73, CD90, and CD105, but did not express CD34, CD45, and CD106. NFSCs also differentiated into adipocytes, osteoblasts, and chondrocytes under appropriate induction conditions. NFSCs released large amounts of growth factors such as VEGF, bFGF, EGF, and others, and growth factor levels increased in a time-dependent manner. At the same time, PRF enhanced proliferation of NFSCs in vitro in a dose-dependent manner, and the growth curves under different concentrations of PRF all showed plateaus 6d after seeding. Facial skin texture was improved to a greater extent after combined injection of nanofat and PRF than after control injection of HA. The nanofat-PRF group had a higher satisfaction rate. Neither treatment caused any complications such as infection, anaphylaxis, or paresthesia during long-term follow-up. Conclusion: NFSCs demonstrate excellent multipotential differentiation and paracrine function, and PRF promotes proliferation of NFSCs during the early stage after seeding. Both nanofat-PRF and HA injection improve facial skin status without serious complications, but the former was associated with greater patient satisfaction, implying that nanofat-PRF injection is a safe, highly effective, and long-lasting method for skin rejuvenation.

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“…Nevertheless, all three types of samples contained 5.1-6.5% of CD34 + cells on the total of all SVF cells, implying that the emulsification/filtration step did not hamper the presence of good numbers of ASCs in the nanofat grafting. The efficacy and the key properties of nanofat have been demonstrated in multiple case studies, concerning skin rejuvenation [83], scar healing [82], skin grafting for wound management [84], and the treatment of vulvar lichen sclerosus (VLS), a chronic inflammatory disease of the anogenital area [85]. The combination of the emulsification technique with the centrifugation phase can yield products highly concentrated in ASCs, eliminating therefore enzymatic digestion, reducing process time and cost, and acting on regulatory constraints [86].…”

Section: Isolation and Differentiation Of Ascsmentioning

confidence: 99%

The Role of Adipose-Derived Stem Cells, Dermal Regenerative Templates, and Platelet-Rich Plasma in Tissue Engineering-Based Treatments of Chronic Skin Wounds

Conese

Annacontini

Carbone

et al. 2020

Stem Cells International

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The continuous improvements in the field of both regenerative medicine and tissue engineering have allowed the design of new and more efficacious strategies for the treatment of chronic or hard-to-heal skin wounds, which represent heavy burden, from a medical and economic point of view. These novel approaches are based on the usage of three key methodologies: stem cells, growth factors, and biomimetic scaffolds. These days, the adipose tissue can be considered the main source of multipotent mesenchymal stem cells, especially adipose-derived stem cells (ASCs). ASCs are easily accessible from various fat depots and show an intrinsic plasticity in giving rise to cell types involved in wound healing and angiogenesis. ASCs can be found in fat grafts, historically used in the treatment of chronic wounds, and have been evaluated as such in both animal models and human trials, to exploit their capability of accelerating wound closure and inducing a correct remodeling of the newly formed fibrovascular tissue. Since survival and fitness of ASCs need to be improved, they are now employed in conjunction with advanced wound dressings, together with dermal regenerative templates and platelet-rich plasma (as a source of growth and healing factors). In this work, we provide an overview of the current knowledge on the topic, based on existing studies and on our own experience.

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The Role of Nanofat Grafting in Vulvar Lichen Sclerosus: A Preliminary Report

Cited by 37 publications

References 7 publications

Development of the Adult Vulvar Lichen Sclerosus Severity Scale—A Delphi Consensus Exercise for Item Generation

Development of the Adult Vulvar Lichen Sclerosus Severity Scale—A Delphi Consensus Exercise for Item Generation

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

The Role of Adipose-Derived Stem Cells, Dermal Regenerative Templates, and Platelet-Rich Plasma in Tissue Engineering-Based Treatments of Chronic Skin Wounds

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