The Role of Nanoparticle Design in Determining Analytical Performance of Lateral Flow Immunoassays

Zhan, Li; Guo, Shuang; Song, Fayi; Gong, Yan; Xu, Feng; Boulware, David R.; McAlpine, Michael C.; Chan, Warren C. W.; Bischof, John C.

doi:10.1021/acs.nanolett.7b02302

Cited by 175 publications

(167 citation statements)

References 37 publications

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“…As smaller Pd−Ir NPs have lower k cat but similar k cat‐specific values compared to larger ones (Figure ), we assume the enhanced detection sensitivities of smaller Pd−Ir NPs based ELISAs can be ascribed to the capture of a great larger number of Pd−Ir NPs during the detection. This assumption is consistent with previous reports that smaller NPs have greater diffusivities and reduced steric effect that make them easier to bind to analytes through the antibody‐antigen interaction …”

Section: Figuresupporting

confidence: 93%

Size Effect in Pd−Ir Core‐Shell Nanoparticles as Nanozymes

Gao

Xia

2020

ChemBioChem

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Comprehensive studies on the size effect in nanozyme (i. e., nanomaterials with enzyme‐like activities)‐based catalysis have rarely been reported. In this work, we systematically investigated the size effect in nanozymes by using Pd−Ir core‐shell nanoparticles with peroxidase‐like activities as a model system. Pd−Ir nanoparticles with four different sizes (3.3, 5.9, 9.8 and 13.0 nm), but identical shapes and surface structures, were designed and synthesized. We found that the catalytic activity for individual nanozymes increased with particle size. The area‐specific catalytic activity was similar for nanoparticles of 3.3–9.8 nm, but decreased slightly when particle size reached 13.0 nm. By using an enzyme‐linked immunosorbent assay (ELISA) as a model platform, the size effect of Pd−Ir nanoparticles in biosensing applications was investigated; smaller nanoparticles were found to offer lower detection limits. This work not only demonstrates the size effect, but also provides an effective strategy to enhance the performance of nanozymes in certain applications.

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Section: Figuresupporting

confidence: 93%

Size Effect in Pd−Ir Core‐Shell Nanoparticles as Nanozymes

Gao

Xia

2020

ChemBioChem

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“…Although RDTs have dominated point-of-care-tests (POCT) for malaria, there have been major concerns about the stability and performance relating to sensitivity and specificity which constrains their impact [25][26][27]. Currently, the commercially available RDTs are about 1000-fold lower in sensitivity than alternative laboratory-based techniques [28][29][30], thus they do not provide the sensitivity and quantitation comparable to the gold-standard microscopy or PCR [29]. Studies have reported that RDTs which incorporate pan-aldolase have poor sensitivity due to low expression of the enzyme by parasites.…”

Section: Parasite Development In Humans Biomarkers and Diagnosismentioning

confidence: 99%

Recent Advances in the Development of Biosensors for Malaria Diagnosis

Krampa

Aniweh

Kanyong

et al. 2020

Sensors

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The impact of malaria on global health has continually prompted the need to develop more effective diagnostic strategies that could overcome deficiencies in accurate and early detection. In this review, we examine the various biosensor-based methods for malaria diagnostic biomarkers, namely; Plasmodium falciparum histidine-rich protein 2 (PfHRP-2), parasite lactate dehydrogenase (pLDH), aldolase, glutamate dehydrogenase (GDH), and the biocrystal hemozoin. The models that demonstrate a potential for field application have been discussed, looking at the fabrication and analytical performance characteristics, including (but not exclusively limited to): response time, sensitivity, detection limit, linear range, and storage stability, which are first summarized in a tabular form and then described in detail. The conclusion summarizes the state-of-the-art technologies applied in the field, the current challenges and the emerging prospects for malaria biosensors.Sensors 2020, 20, 799 2 of 21 emergence and spread of drug resistance. It could also reduce the pool of individuals who can contribute to malaria transmission [5].To date, many technologies have attempted to circumvent the challenges in malaria diagnostics with technologies that address point-of-care needs and early stage asymptomatic detection. In this review, we first comprehensively summarize the biomarkers targeted during the course of malaria with emphasis on the importance of sensitive early detection. Next, we provide an overview of the recent advances in biosensor technologies for the detection of the most targeted biomarkers, focusing on: development, analytical performances, and suitability for point of care testing. The prevailing challenges and future outlook of the use of these technologies in the field are also highlighted. Parasite Development in Humans, Biomarkers, and DiagnosisThe developmental cycle of Plasmodium species that infect humans is briefly illustrated in Figure 1 [6]. The cycle begins with the injection of sporozoites into the host's circulation by an infected female Anopheles mosquito. The sporozoites then target and enter hepatocytes where they multiply and differentiate into merozoites. This stage of the parasite life cycle is known as pre-erythrocytic. In infections involving P. vivax and P. ovale, dormant forms of the liver stage, called hypnozoites may persist in the liver [7] and cause relapse of the infection, thereby making it difficult to eradicate. The pre-erythrocytic stage is essential in the establishment of malaria infection. This stage is asymptomatic, however, it is difficult for diagnostic tools to detect sporozoites because hepatocytes invasion occurs within 30-45 min after sporozoites are inoculated by the infected mosquito [8,9]. This short time and low numbers of sporozoites injected leaves little time for their detection. Some efforts in finding biomarkers for detection of early liver stage or the dormant form have identified the Plasmodium liver-specific protein 2 (LISP2) [10,11]. The sporozoite surface circum...

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“…To improve the analytical sensitivity and detection range of the Au NP-based LFA, Bischof et al investigated the effect of different size of Au NPs (30, 60, and 100 nm) on the performance of LFA devices [186]. They found that the sensitivity was significantly dependent on the optical properties of Au NPs and their binding efficiency.…”

Section: Plasmonic Paper Devicesmentioning

confidence: 99%

Plasmonic molecular assays: Recent advances and applications for mobile health

Wei

2018

Nano Res.

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Plasmonics-based biosensing assays have been extensively employed for biomedical applications. Significant advancements in use of plasmonic assays for the construction of point-of-care (POC) diagnostic methods have been made to provide effective and urgent health care of patients, especially in resourcelimited settings. This rapidly progressive research area, centered on the unique surface plasmon resonance (SPR) properties of metallic nanostructures with exceptional absorption and scattering abilities, has greatly facilitated the development of cost-effective, sensitive, and rapid strategies for disease diagnostics and improving patient healthcare in both developed and developing worlds. This review highlights the recent advances and applications of plasmonic technologies for highly sensitive protein and nucleic acid biomarker detection. In particular, we focus on the implementation and penetration of various plasmonic technologies in conventional molecular diagnostic assays, and discuss how such modification has resulted in simpler, faster, and more sensitive alternatives that are suited for point-of-use. Finally, integration of plasmonic molecular assays with various portable POC platforms for mobile health applications are highlighted.

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The Role of Nanoparticle Design in Determining Analytical Performance of Lateral Flow Immunoassays

Cited by 175 publications

References 37 publications

Size Effect in Pd−Ir Core‐Shell Nanoparticles as Nanozymes

Size Effect in Pd−Ir Core‐Shell Nanoparticles as Nanozymes

Recent Advances in the Development of Biosensors for Malaria Diagnosis

Plasmonic molecular assays: Recent advances and applications for mobile health

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