In this study, eco-friendly natural coloring matter such as pomegranate, curcumin, cutch, red onion peel and a mixture of red onion peel/curcumin (40 g/L, 50%) are used. They are extracted in water at 80°C (red onion at boiling). Cotton, wool, silk and nylon fabrics are dyed using conventional method at pH 4 (except cotton at pH 8), liquor ratio 1:40 and 25 g/L dye concentration for a specific time in presence or absence of FeSO4 using simultaneous mordanting method. Colour strength (K/S) and colorimetric data (L*, a* and b*) are measured at specific λ max . Also, fastness properties (light, washing, rubbing) are determined. Also, ultraviolet protection and antibacterial activity of fabrics against micro-organisms strains of Staphylococcus aureus, Klebsiella Pneumoniae and Candida albicans are measured. Results of the dyed fabrics are highly UV protective and have effective antibacterial activity. All the above dyed fabrics are suggested to use in the medical cloths.