The role of new agents in the treatment of non-small cell lung cancer

Bröker, Linda E.; Giaccone, Giuseppe

doi:10.1016/s0959-8049(02)00457-4

Cited by 34 publications

(27 citation statements)

References 141 publications

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“…The number of lung cancer patients are increasing annually. Therapeutic approaches, such as chemotherapy, radiotherapy, and surgery have been widely used to treat lung cancer; however, these treatments offered little survival rate due to severe toxicity and drug resistance (Broker et al, 2002;Singh et al, 2008). Therefore, understanding of the carcinogenic mechanisms and genes involved in lung cancer cell's fast growth is of much importance in developing more precise treatments compared to conventional treatment methods.…”

Section: Introductionmentioning

confidence: 99%

Silencing of the COPS3 Gene by siRNA Reduces Proliferation of Lung Cancer Cells Most Likely via induction of Cell Cycle Arrest and Apoptosis

Wang

Cui²,

Li³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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The COPS3 gene has stimulating effect on cell proliferation and progression of osteosarcomas and related cells. However, the features of COPS3 and its potential application as a therapeutic target in other cancers has not yet been studied. In this study, therefore, the effect of COPS3 silencing via COPS3 siRNA on lung cancer cell proliferation was examined. Expression levels of COPS3 gene in COPS3 siRNA infected cells and control siRNA infected cells were compared with real time PCR and Western blot analysis. Cell proliferation levels were comprehensively analyzed by MTT, BrdU incorporationy, and colony formation assays. For mechanistic assessment the effects of COPS3 silencing on cell cycle and apoptosis were analyzed using flow cytometry. Results showed that successful silencing of the COPS3 gene at both translational and transcriptional levels significantly reduced the proliferation and colony formation by lung cancer cells (p<0.01). Flow cytometry showed cell cycle arrest in the G0/G1 phase after COPS3 silencing, and more importantly, apoptosis was induced as a result of COPS3 knockdown, which negatively affected cell survival. Therefore, these results provide another piece of important evidence that the COPS3 gene expressed in lung cancer cells may play a critical role in stimulating proliferation. Down-regulation of COPS3 could significantly inhibit lung cancer cell growth, which was most likely mediated via induction of cell cycle arrest in G0/G1 phase and apoptosis.

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Section: Introductionmentioning

confidence: 99%

Silencing of the COPS3 Gene by siRNA Reduces Proliferation of Lung Cancer Cells Most Likely via induction of Cell Cycle Arrest and Apoptosis

Wang

Cui²,

Li³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…In view of the fact that only one patient received more than four cycles of therapy, no conclusions on the cardiotoxic potential of the drug can be drawn at this time. The issue of cardiotoxicity will therefore be re-addressed in forthcoming phase II trials (Broker and Giaccone, 2002).…”

mentioning

confidence: 99%

Anthracyclines: Molecular Advances and Pharmacologic Developments in Antitumor Activity and Cardiotoxicity

et al. 2004

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“…Por esta razão, as epotilonas apresentam atividade superior ao paclitaxel em células resistentes, sendo que estes resultados foram, posteriormente, confirmados por cientistas da GBF 15 . Após os bons resultados preliminares, a epotilona B passou com sucesso por testes clínicos de fase I, estando atualmente em fase II 11,18 .…”

Section: Epotilonasunclassified

Novos produtos naturais capazes de atuar na estabilização de microtúbulos, um importante alvo no combate ao câncer

Souza¹

2004

Quím. Nova

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The role of new agents in the treatment of non-small cell lung cancer

Cited by 34 publications

References 141 publications

Silencing of the COPS3 Gene by siRNA Reduces Proliferation of Lung Cancer Cells Most Likely via induction of Cell Cycle Arrest and Apoptosis

Silencing of the COPS3 Gene by siRNA Reduces Proliferation of Lung Cancer Cells Most Likely via induction of Cell Cycle Arrest and Apoptosis

Anthracyclines: Molecular Advances and Pharmacologic Developments in Antitumor Activity and Cardiotoxicity

Novos produtos naturais capazes de atuar na estabilização de microtúbulos, um importante alvo no combate ao câncer

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