The Role of Non-Coding RNAs in Cytoplasmic Male Sterility in Flowering Plants

Štorchová, Helena

doi:10.3390/ijms18112429

Cited by 18 publications

(7 citation statements)

References 94 publications

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“…We raise the question as to whether each of these Rf s is an idiosyncratic example that episodically emerged during plant-species diversification or is there a tendency or trend underlying Rf evolution. To approach this question, we will briefly review plant Rf s. Recent reviews on CMS, Rf and other types of male sterility will help to understand the progress of this research area [19][20][21][22][23][24][25][26][27][28][29][30][31][32]. This question has also been addressed from the viewpoint of evolutionary genetics (reviewed ib [33]).…”

Section: Introductionmentioning

confidence: 99%

What Does the Molecular Genetics of Different Types of Restorer-of-Fertility Genes Imply?

Kubo

Arakawa

Honma

et al. 2020

Plants

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Cytoplasmic male sterility (CMS) is a widely used trait for hybrid seed production. Although male sterility is caused by S cytoplasm (male-sterility inducing mitochondria), the action of S cytoplasm is suppressed by restorer-of-fertility (Rf), a nuclear gene. Hence, the genetics of Rf has attained particular interest among plant breeders. The genetic model posits Rf diversity in which an Rf specifically suppresses the cognate S cytoplasm. Molecular analysis of Rf loci in plants has identified various genes; however, pentatricopeptide repeat (PPR) protein (a specific type of RNA-binding protein) is so prominent as the Rf-gene product that Rfs have been categorized into two classes, PPR and non-PPR. In contrast, several shared features between PPR- and some non-PPR Rfs are apparent, suggesting the possibility of another grouping. Our present focus is to group Rfs by molecular genetic classes other than the presence of PPRs. We propose three categories that define partially overlapping groups of Rfs: association with post-transcriptional regulation of mitochondrial gene expression, resistance gene-like copy number variation at the locus, and lack of a direct link to S-orf (a mitochondrial ORF associated with CMS). These groups appear to reflect their own evolutionary background and their mechanism of conferring S cytoplasm specificity.

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Section: Introductionmentioning

confidence: 99%

What Does the Molecular Genetics of Different Types of Restorer-of-Fertility Genes Imply?

Kubo

Arakawa

Honma

et al. 2020

Plants

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“…Although the exact nature of miRNAs with respect to CMS in plants remains elusive, discovery of miRNAs with possible implications for CMS induction is intriguing in this respect. The recent explosion of high-throughput sequencing has enabled genome-wide discovery of these regulatory molecules and their targets, implying their potential involvement in CMS [67]. Using this method combined with bioinformatic analysis, researchers have successively identified differentially expressed miRNAs and their targets between CMS lines and fertile lines of rice, B. juncea, cybrid pummelo (Citrus grandis) and many other species [45][46][47][48][49][50][51].…”

Section: Plos Onementioning

confidence: 99%

Identification of microRNAs and their targets in inflorescences of an Ogura-type cytoplasmic male-sterile line and its maintainer fertile line of turnip (Brassica rapa ssp. rapifera) via high-throughput sequencing and degradome analysis

Lin

Jin

et al. 2020

PLoS ONE

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Cytoplasmic male sterility (CMS) is a widely used trait in angiosperms caused by perturbations in nucleus-mitochondrion interactions that suppress the production of functional pollen. MicroRNAs (miRNAs) are small non-coding RNAs that act as regulatory molecules of transcriptional or post-transcriptional gene silencing in plants. The discovery of miRNAs and their possible implications in CMS induction provides clues for the intricacies and complexity of this phenomenon. Previously, we characterized an Ogura-CMS line of turnip (Brassica rapa ssp. rapifera) that displays distinct impaired anther development with defective microspore production and premature tapetum degeneration. In the present study, high-throughput sequencing was employed for a genome-wide investigation of miRNAs. Six small RNA libraries of inflorescences collected from the Ogura-CMS line and its maintainer fertile (MF) line of turnip were constructed. A total of 120 pre-miRNAs corresponding to 89 mature miR-NAs were identified, including 87 conversed miRNAs and 33 novel miRNAs. Among these miRNAs, the expression of 10 differentially expressed mature miRNAs originating from 12 pre-miRNAs was shown to have changed by more than twofold between inflorescences of the Ogura-CMS line and inflorescences of the MF line, including 8 down-and 2 up-regulated miRNAs. The expression profiles of the differentially expressed miRNAs were confirmed by stem-loop quantitative real-time PCR. In addition, to identify the targets of the identified miR-NAs, a degradome analysis was performed. A total of 22 targets of 25 miRNAs and 17 targets of 28 miRNAs were identified as being involved in the reproductive development for Ogura-CMS and MF lines of turnip, respectively. Negative correlations of expression patterns between partial miRNAs and their targets were detected. Some of these identified targets, such as squamosa promoter-binding-like transcription factor family proteins, auxin response factors and pentatricopeptide repeat-containing proteins, were previously

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“…In this Special Issue, “Plant Mitochondria”, a total of 19 articles were accepted with 15 original research articles and 4 review articles broadly covering the field of plant mitochondrial research (Table 1). Manuscripts focused on protein synthesis and degradation [3,4,5,6], abiotic stress [7,8,9,10], OxPhos [11,12,13,14], protein import [15,16,17], ROS and antioxidants [18], and CMS [19,20].…”

mentioning

confidence: 99%

“…Both GMS and CMS are widely used in agricultural production for the production of hybrid crops that benefit from heterosis. In this Special Issue Štorchová [20] presents a comprehensive review of the role of non-coding RNA in the CMS of flowering plants, while Reddemann and Horn [19] presented research examining the role of atp9 in the male sterility of CMS PET2 in sunflower. Here they showed that CMS PET2, which has the potential to become an alternative CMS source for commercial breeding, has a duplicated atp9 with a 271-bp-insertion in the 5’ region of one of the atp9 genes which results in two unique open reading frames ( orf288 and orf231 ).…”

mentioning

confidence: 99%