“…In addition, we have reported that patients with protein overexpression of actinin-4 had a worse prognosis than patients without overexpression in breast (4,12), pancreas (16,17), ovarian (13)(14)(15), thyroid (18), salivary gland (19), and tongue cancers (20). Moreover, involvement in malignant phenotypes for protein overexpression of actinin-4 was reported in brain tumors (21)(22)(23), head and neck cancer (24,25), lung cancer (26)(27)(28), breast cancer (29)(30)(31), esophageal cancer (32), gastric cancer (33), pancreatic cancer (34), gastrointestinal stromal tumor (GIST) (35), cervical cancer (36), ovarian cancer, bladder cancer (37), prostate cancer (38,39), melanoma (40,41), leukemia (42,43), and osteosarcoma (44,45) from not only our groups, but also other independent groups. In fact, use of an exogenous transfection technique to overexpress actinin-4 in cancer cells demonstrated that cancer cells overexpressing actinin-4 can form the protrusions that are involved in cell motility and cancer invasion and exhibit significantly increased invasive potential (6).…”