Nafion, a sulfonated tetrafluoroethylene copolymer, consists of a hydrophobic fluorocarbon backbone (-CF2-CF2-) to which hydrophilic sulfonate groups ( SO −3 ) are attached. The presence of negatively charged sulfonate groups in a nanomembrane brings about a high level of proton conductivity. In this study, Saccharomyces cerevisiae was used for production of bioelectricity in a 2-chambered microbial fuel cell (MFC). We selected 9-cm 2 Nafion 117 and Nafion 112 as nanomembranes to transport the produced proton from the anode chamber to the cathode compartment at ambient temperature and pressure. Initial glucose concentration was 30 g/L. The maximum obtained voltage, current, and power density for Nafion 117 were 668 mV, 60.28 mA/m 2 , and 9.95 mW/m 2 , respectively. For Nafion 112, those results were 670 mV, 150.6 mA/m 2 , and 31.32 mW/m 2 , respectively.