The Role Of OprD Gene In The Carbapenems Resistance In Pseudomonas Aeruginosa Isolated From Burns Infection In Al-Najaf Province

Abstract

Carbapenems-resistant Pseudomonas aeruginosa mutants are disseminating around the globe. 90%-100% of P. aeruginosa isolates became Carbapenems-resistant mutants in some countries like Thailand and Brazil respectively. Finding alternative therapeutic options instead of carbapenems is the global duty of researchers and institutions. The OprD gene mutation/s has the main role in carbapenems resistance appearance in P. aeruginosa due to the decrease in the outer membrane permeability for carbapenems. Aim: This study aims to assess the antimicrobial activity of carbapenems (imipenem, and meropenem) against Pseudomonas aeruginosa isolated from hospitalized burn patients in Al-Najaf/Iraq, and the role of OprD gene mutations in the resistance. Methods and materials: 76 samples were collected from hospitalized burn patients suffering from burn wounds infections, and the identity of each cultivated microbe was confirmed by VITEK compact system 2 and further confirmation for P. aeruginosa isolates via performing molecular identification by OprD gene detection, 10/12 carbapenems resistant isolates, 1/12 sensitive, and 1/12 intermediate resistant were submitted to OprD gene sequencing by Sanger method

Molecular Detection of Carbapenem's Genes Among (Pseudomonas Areuginosa) Isolates

“…Descriptions of the susceptibility of P. aeruginosa to piperacillin have ranged from as low as 33.7% and 60.5. Similarly, [14] reported a moderate resistance rate against piperacillin among P. aeruginosa strains isolated.…”