The Role of Outer Membrane Proteins in UPEC Antimicrobial Resistance: A Systematic Review

Rodrigues, Inês; Rodrigues, Silvia Cristina Martini; Duarte, Filipe V.; Costa, Paula M. da; Costa, Paulo Martins da

doi:10.3390/membranes12100981

Cited by 12 publications

(8 citation statements)

References 63 publications

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“…Reducing antibiotic influx through OMP channels is another important mechanism of drug resistance in bacteria ( Rodrigues et al, 2022 ). For example, in E. coli , OmpC is associated with β-lactams resistance, OmpF with β-lactams and fluoroquinolone resistance, and TolC, YddB, OmpX and TosA with the resistances of enrofloxacin, novobiocin, fluoroquinolones, β-lactams and globomycin ( Rodrigues et al, 2022 ). Seventeen OMPs including OmpU, OmpN, OmpA, OmpV and OmpK are associated with the resistance of V. alginolyticus to erythromycin, tetracycline, kanamycin, streptomycin, nalidixic acid and chloromycetin ( Xiong et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of sublethal dose of chloramphenicol on biofilm formation and virulence in Vibrio parahaemolyticus

Zhang,

Cai,

Luo

et al. 2023

Front. Microbiol.

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Vibrio parahaemolyticus isolates are generally very sensitive to chloramphenicol. However, it is usually necessary to transfer a plasmid carrying a chloramphenicol resistance gene into V. parahaemolyticus to investigate the function of a specific gene, and the effects of chloramphenicol on bacterial physiology have not been investigated. In this work, the effects of sublethal dose of chloramphenicol on V. parahaemolyticus were investigated by combined utilization of various phenotypic assays and RNA sequencing (RNA-seq). The results showed that the growth rate, biofilm formation capcity, c-di-GMP synthesis, motility, cytoxicity and adherence activity of V. parahaemolyticus were remarkably downregulated by the sublethal dose of chloramphenicol. The RNA-seq data revealed that the expression levels of 650 genes were significantly differentially expressed in the response to chloramphenicol stress, including antibiotic resistance genes, major virulence genes, biofilm-associated genes and putative regulatory genes. Majority of genes involved in the synthesis of polar flagellum, exopolysaccharide (EPS), mannose-sensitive haemagglutinin type IV pilus (MSHA), type III secretion systems (T3SS1 and T3SS2) and type VI secretion system 2 (T6SS2) were downregulated by the sublethal dose of chloramphenicol. Five putative c-di-GMP metabolism genes were significantly differentially expressed, which may be the reason for the decrease in intracellular c-di-GMP levels in the response of chloramphenicol stress. In addition, 23 genes encoding putative regulators were also significantly differentially expressed, suggesting that these regulators may be involved in the resistance of V. parahaemolyticus to chloramphenicol stress. This work helps us to understand how chloramphenicol effect on the physiology of V. parahaemolyticus.

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Section: Discussionmentioning

confidence: 99%

Effect of sublethal dose of chloramphenicol on biofilm formation and virulence in Vibrio parahaemolyticus

Zhang,

Cai,

Luo

et al. 2023

Front. Microbiol.

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“…The function of particular porins for antibiotic transport is only marginally significant, save from specific examples like YddB and LamB. Most non β -lactam antibiotics appear to penetrate the OM in a porin-independent manner, but the majority of β -lactam antibiotics are transported with non-specific porins, particularly OmpF and OmpC ( Choi and Lee, 2019 ; Rodrigues et al., 2022 ).…”

Section: Membrane Permeability Mechanismsmentioning

confidence: 99%

A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli

Nasrollahian,

Graham,

Halaji

2024

Front. Cell. Infect. Microbiol.

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The dissemination of antibiotic resistance in Escherichia coli poses a significant threat to public health worldwide. This review provides a comprehensive update on the diverse mechanisms employed by E. coli in developing resistance to antibiotics. We primarily focus on pathotypes of E. coli (e.g., uropathogenic E. coli) and investigate the genetic determinants and molecular pathways that confer resistance, shedding light on both well-characterized and recently discovered mechanisms. The most prevalent mechanism continues to be the acquisition of resistance genes through horizontal gene transfer, facilitated by mobile genetic elements such as plasmids and transposons. We discuss the role of extended-spectrum β-lactamases (ESBLs) and carbapenemases in conferring resistance to β-lactam antibiotics, which remain vital in clinical practice. The review covers the key resistant mechanisms, including: 1) Efflux pumps and porin mutations that mediate resistance to a broad spectrum of antibiotics, including fluoroquinolones and aminoglycosides; 2) adaptive strategies employed by E. coli, including biofilm formation, persister cell formation, and the activation of stress response systems, to withstand antibiotic pressure; and 3) the role of regulatory systems in coordinating resistance mechanisms, providing insights into potential targets for therapeutic interventions. Understanding the intricate network of antibiotic resistance mechanisms in E. coli is crucial for the development of effective strategies to combat this growing public health crisis. By clarifying these mechanisms, we aim to pave the way for the design of innovative therapeutic approaches and the implementation of prudent antibiotic stewardship practices to preserve the efficacy of current antibiotics and ensure a sustainable future for healthcare.

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“…Outer membrane proteins (OMPs) are a major source of virulence in gram-negative bacteria making them a potential candidate for the development of mAb [ 93 , 94 , 95 , 96 , 97 ]. Located abundantly on the outer membrane, OMPs are proteinaceous components mostly composed of β-barrel [ 98 ].…”

Section: Current Therapeutic Approaches To Treat Sepsismentioning

confidence: 99%

Recent Advances in Monoclonal Antibody-Based Approaches in the Management of Bacterial Sepsis

2023

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Sepsis is a life-threatening condition characterized by an uncontrolled inflammatory response to an infectious agent and its antigens. Immune cell activation against the antigens causes severe distress that mediates a strong inflammatory response in vital organs. Sepsis is responsible for a high rate of morbidity and mortality in immunosuppressed patients. Monoclonal antibody (mAb)-based therapeutic strategies are now being explored as a viable therapy option for severe sepsis and septic shock. Monoclonal antibodies may provide benefits through two major strategies: (a) monoclonal antibodies targeting the pathogen and its components, and (b) mAbs targeting inflammatory signaling may directly suppress the production of inflammatory mediators. The major focus of mAb therapies has been bacterial endotoxin (lipopolysaccharide), although other surface antigens are also being investigated for mAb therapy. Several promising candidates for mAbs are undergoing clinical trials at present. Despite several failures and the investigation of novel targets, mAb therapy provides a glimmer of hope for the treatment of severe bacterial sepsis and septic shock. In this review, mAb candidates, their efficacy against controlling infection, with special emphasis on potential roadblocks, and prospects are discussed.

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The Role of Outer Membrane Proteins in UPEC Antimicrobial Resistance: A Systematic Review

Cited by 12 publications

References 63 publications

Effect of sublethal dose of chloramphenicol on biofilm formation and virulence in Vibrio parahaemolyticus

Effect of sublethal dose of chloramphenicol on biofilm formation and virulence in Vibrio parahaemolyticus

A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli

Recent Advances in Monoclonal Antibody-Based Approaches in the Management of Bacterial Sepsis

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