The role of passive calcium influx through the cell membrane in galvanotaxis

Borys, Przemysław

doi:10.2478/s11658-013-0082-3

Cited by 7 publications

(7 citation statements)

References 28 publications

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“…The molecular processes that govern cell behavior under galvanotactic conditions are not well understood. However, it is known that different mechanisms are involved in this behavior, for example, the bidirectional traffic of Ca 2+ through the cell membranes, the sequential events of actin polymerization/depolymerization and the actomyosin contractility [54][55][56].…”

Section: External Stimuli Migration and Cancermentioning

confidence: 99%

Cell Motility and Cancer

Fuente

López

2020

Cancers

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Cell migration is an essential systemic behavior, tightly regulated, of all living cells endowed with directional motility that is involved in the major developmental stages of all complex organisms such as morphogenesis, embryogenesis, organogenesis, adult tissue remodeling, wound healing, immunological cell activities, angiogenesis, tissue repair, cell differentiation, tissue regeneration as well as in a myriad of pathological conditions. However, how cells efficiently regulate their locomotion movements is still unclear. Since migration is also a crucial issue in cancer development, the goal of this narrative is to show the connection between basic findings in cell locomotion of unicellular eukaryotic organisms and the regulatory mechanisms of cell migration necessary for tumor invasion and metastases. More specifically, the review focuses on three main issues, (i) the regulation of the locomotion system in unicellular eukaryotic organisms and human cells, (ii) how the nucleus does not significantly affect the migratory trajectories of cells in two-dimension (2D) surfaces and (iii) the conditioned behavior detected in single cells as a primitive form of learning and adaptation to different contexts during cell migration. New findings in the control of cell motility both in unicellular organisms and mammalian cells open up a new framework in the understanding of the complex processes involved in systemic cellular locomotion and adaptation of a wide spectrum of diseases with high impact in the society such as cancer.

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Section: External Stimuli Migration and Cancermentioning

confidence: 99%

Cell Motility and Cancer

Fuente

López

2020

Cancers

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“…In general, several theories and mechanisms on how EFs affect cells directly are debated. 46 Fig. 2 shows an overview of our present understanding of the mechanism underlying cell electrotactic behaviour.…”

Section: Mechanism Of Intracellular Motility Pathwaysmentioning

confidence: 99%

“…63 The role of calcium ions in the motility process leads to various possible calcium pathways into the cytoplasm voltage-gated calcium channels (VGCCs), Na + /Ca 2+ exchangers (NCX), plasma membrane Ca 2+ ATP-ases (PMCAs), leakage channels, internal rearrangements by calcium ''waves,'' or eventually, calcium release (uptake) from internal stores. 46,64 Other intracellular signalling cascades reported in electrotaxis include PI3K, cAMP, PTEN, ERK1/2. [65][66][67][68] Voltage-gated Ca 2+ channels play a critical role in the control of selective Ca 2+ flow down their electrochemical gradient in response to a change in the membrane potential in various cells.…”

Section: Calcium Signalling Rolementioning

confidence: 99%

“…Some researchers purported that cells migrating toward the cathode have a cathode high [Ca 2+ ] i on the leading edge of the cell. 11,40,46,54 This side of the cell then contracts, thereby propelling the cell toward the cathode. This process continues until the voltage-gated Ca 2+ channels near the cathodal side depolarise (open), thereby allowing Ca 2+ influx.…”

Section: Calcium Signalling Rolementioning

confidence: 99%

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Influence of electrotaxis on cell behaviour

et al. 2014

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Understanding the mechanism of cell migration and interaction with the microenvironment is not only of critical significance to the function and biology of cells, but also has extreme relevance and impact on physiological processes and diseases such as morphogenesis, wound healing, neuron guidance, and cancer metastasis. External guidance factors such as topography and physical cues of the microenvironment promote directional migration and can target specific changes in cell motility and signalling mechanisms. Recent studies have shown that cells can directionally respond to applied electric fields (EFs), in both in vitro and in vivo settings, a phenomenon called electrotaxis. However, the exact cellular mechanisms for sensing electrical signals are still not fully well understood, and it is thus far unknown how cells recognize and respond to electric fields, although some studies have suggested that electro-migration of some cell surface receptors and ion channels in cells could be involved. Applied electric fields may have a potential clinical role in guiding cell migration and present a more precise manageability to change the magnitude and direction of the electric field than most other guidance cues such as chemical cues. Here we present a review of recent studies used for studying electrotaxis to point out similarities, identify points of disagreement, and stimulate new directions for investigation. Insights into the mechanisms by which applied EFs direct cell migration, morphological change and development will enable current and future therapeutic applications to be optimized.

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“…Calcium ion flux is known to be involved in the electrotaxis signaling of multiple cell types including mouse fibroblasts, human prostate cancer cell, and neural crest cells [65][66][67][68][69] . Deregulation of calcium influx in the cells reduces actin polymerization and affects cell motility speed but its effect on electrotactic directedness vary depending on cell types 65,69 .…”

Section: Glioblastoma Electrotaxis Requires Extracellular Calciummentioning

confidence: 99%

Voltage-gated ion channels mediate the electrotaxis of glioblastoma cells in a hybrid PMMA/PDMS microdevice

Tsai

IJspeert

Shen

2020

Preprint

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Transformed astrocytes in the most aggressive form cause glioblastoma, the most common cancer in central nervous system with high mortality. The physiological electric field by neuronal local field potentials and tissue polarity may guide the infiltration of glioblastoma cells through the electrotaxis process. However, microenvironments with multiplex gradients are difficult to create. In this work, we have developed a hybrid microfluidic platform to study glioblastoma electrotaxis in controlled microenvironments with high throughput quantitative analysis by a machine learning-powered single cell tracking software. By equalizing the hydrostatic pressure difference between inlets and outlets of the microchannel, uniform single cells can be seeded reliably inside the microdevice. The electrotaxis of two glioblastoma models, T98G and U-251MG, require optimal laminin-containing extracellular matrix and exhibits opposite directional and electro-alignment tendencies. Calcium signaling is a key contributor in glioblastoma pathophysiology but its role in glioblastoma electrotaxis is still an open question. Anodal T98G electrotaxis and cathodal U-251MG electrotaxis require the presence of extracellular calcium cations. U-251MG electrotaxis is dependent on the P/Q-type voltage-gated calcium channel (VGCC) and T98G is dependent on the R-type VGCC. U-251MG and T98G electrotaxis are also mediated by A-type (rapidly inactivating) voltage-gated potassium channels and acidsensing sodium channels. The involvement of multiple ion channels suggests that the glioblastoma electrotaxis is complex and patient-specific ion channel expression can be critical to develop personalized therapeutics to fight against cancer metastasis. The hybrid microfluidic design and machine learning-powered single cell analysis provide a simple and flexible platform for quantitative investigation of complicated biological systems.

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The role of passive calcium influx through the cell membrane in galvanotaxis

Cited by 7 publications

References 28 publications

Cell Motility and Cancer

Cell Motility and Cancer

Influence of electrotaxis on cell behaviour

Voltage-gated ion channels mediate the electrotaxis of glioblastoma cells in a hybrid PMMA/PDMS microdevice

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