The role of phenylacetic acid in biosynthesis of penicillin amidase in E. coli

Babu, Punuri Jayasekhar; Panda, T.

doi:10.1007/bf00369280

Cited by 7 publications

(2 citation statements)

References 5 publications

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“…Obviously, there is no repression by glucose, no induction and stimulation by phenylacetic acid and no thermoregulation. These three kinds of regulation were described repeatedly in PAC producer strains of E. coli containing a vector or not (BABU and PANDA 1991, DAUMY et af. 1982, ROBAS et al 1993, ROBAS and BRANLANT 1994, VOJTISEK and SLEZAK 1975.…”

Section: Discussionmentioning

confidence: 77%

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Synthesis and secretion of recombinant penicillin G acylase in bacterial L‐forms

Gumpert¹,

Cron

Plapp

et al. 1996

J. Basic Microbiol.

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L‐form strains of Proteus mirabilis and Escherichia coli lacking the cell wall represent an alternative prokaryotic cell system for the production of recombinant proteins (KLESSEN et al. 1988, LAPLACE et al. 1988 a, 1989b). We could demonstrate that they are also able to synthesize the enzyme penicillin G acylase (PAC)1). PAC was processed and secreted into the medium by recombinant L‐form strains. The synthesis of PAC was growth‐associated and stably regulated. Expression, secretion, and processing were not temperature‐dependent and occurred at 26 °C, 32 °C and even 37 °C. The expression vector pHC1 carried the pac gene under the control of the lac UV promoter and a kanamycin resistance gene. It could be maintained in L‐form cells, showing low structural as well as segregational instability. The secretion of the biologically active enzyme into the medium indicated that the postranslational processing of the PAC molecule, including the excision of a 54 amino acid spacer peptide between the α and β subunit, is not carried out in the periplasmic space, but occurs at the cytoplasmic membrane or autocatalytically.

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Section: Discussionmentioning

confidence: 77%

“…Phenylacetic acid and ammonium phenylacetate in concentrations of 0.1-2% are known as highly effective inducers of natural PAC activity in E. coli (DAUMY et al 1982, VOJTISEK and SLEZAK 1975, BABU and PANDA 1991, ROBAS et al 1993. For the strain l?…”

Section: Medium Compositionmentioning

confidence: 99%