The Role of Physical Stimuli on Calcium Channels in Chondrogenic Differentiation of Mesenchymal Stem Cells

Uzielienè, Ilona; Bernotas, Paulius; Mobasheri, Ali; Bernotienė, Eiva

doi:10.3390/ijms19102998

Cited by 56 publications

(58 citation statements)

References 62 publications

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“…During this process, progenitor cells differentiate into chondrocytes which later undergo maturation and mineralization, finally resulting in new bone tissue (reviewed in [95]). Whereas the positive effects of EStim on endochondral ossification have been shown in previous studies [29,96,97], there few studies focused on analyzing the effects EStim has on endochondral ossification and MSC chondrogenic differentiation (reviewed in [98]). In our own in vitro studies, we were not able to detect a positive effect of direct current EStim on rat MSC chondrogenic differentiation (unpublished data).…”

Section: Chondrogenesismentioning

confidence: 99%

Electrical stimulation in bone tissue engineering treatments

Leppik

Oliveira

Bhavsar

et al. 2020

Eur J Trauma Emerg Surg

161

148

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Electrical stimulation (EStim) has been shown to promote bone healing and regeneration both in animal experiments and clinical treatments. Therefore, incorporating EStim into promising new bone tissue engineering (BTE) therapies is a logical next step. The goal of current BTE research is to develop combinations of cells, scaffolds, and chemical and physical stimuli that optimize treatment outcomes. Recent studies demonstrating EStim's positive osteogenic effects at the cellular and molecular level provide intriguing clues to the underlying mechanisms by which it promotes bone healing. In this review, we discuss results of recent in vitro and in vivo research focused on using EStim to promote bone healing and regeneration and consider possible strategies for its application to improve outcomes in BTE treatments. Technical aspects of exposing cells and tissues to EStim in in vitro and in vivo model systems are also discussed.

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Section: Chondrogenesismentioning

confidence: 99%

Electrical stimulation in bone tissue engineering treatments

Leppik

Oliveira

Bhavsar

et al. 2020

Eur J Trauma Emerg Surg

161

148

View full text Add to dashboard Cite

show abstract

“…Growth factors such as TGF-β, IGF, and bone morphogenetic proteins (BMP)-2,-4,-7 are necessary to stimulate chondrogenic processes and require the presence of calcium ions (Ca 2+ ) to regulate cell functions, such as the synthesis of extracellular matrix components. Physical stimuli have been associated with the regulation of Ca 2+ entry, primarily through voltage-operated calcium channels (VOCCs), transient receptor potential (TRP) channels, and purinergic receptors [22]. Furthermore, VOCC inhibitors have shown to reduce cartilage degradation and the progression of osteoarthritis [23], suggesting their importance in both physiological and pathological milieux.…”

Section: Influencing the Mechanical Environmentmentioning

confidence: 99%

New Insights on Mechanical Stimulation of Mesenchymal Stem Cells for Cartilage Regeneration

et al. 2020

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Successful tissue regeneration therapies require further understanding of the environment in which the cells are destined to be set. The aim is to structure approaches that aspire to a holistic view of biological systems and to scientific reliability. Mesenchymal stem cells represent a valuable resource for cartilage tissue engineering, due to their chondrogenic differentiation capacity. Promoting chondrogenesis, not only by growth factors but also by exogenous enhancers such as biomechanics, represents a technical enhancement. Tribological evaluation of the articular joint has demonstrated how mechanical stimuli play a pivotal role in cartilage repair and participate in the homeostasis of this tissue. Loading stresses, physiologically experienced by chondrocytes, can upregulate the production of proteins like glycosaminoglycan or collagen, fundamental for articular wellness, as well as promote and preserve cell viability. Therefore, there is a rising interest in the development of bioreactor devices that impose compression, shear stress, and hydrostatic pressure on stem cells. This strategy aims to mimic chondrogenesis and overcome complications like hypertrophic phenotyping and inappropriate mechanical features. This review will analyze the dynamics inside the joint, the natural stimuli experienced by the chondrocytes, and how the biomechanical stimuli can be applied to a stem cell culture in order to induce chondrogenesis.

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“…To date, ion concentration in the spheroids has not been explored. It has been reported that the mechanical stress affected the activation of calcium ion channels on the cell membrane of MSCs, and the calcium ion-involved signaling played important roles in the differentiation of MSCs [68,69]. On the other hand, the cytoskeleton would be dramatically rearranged during the spheroid formation on CS substrates.…”

Section: Physical and Chemical Factors Affecting Cell Behaviors In Scmentioning

confidence: 99%

Cellular Spheroids of Mesenchymal Stem Cells and Their Perspectives in Future Healthcare

2019

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Intrinsic cellular properties of several types of cells are dramatically altered as the culture condition shifts from two-dimensional (2D) to three-dimensional (3D) environment. Currently, several lines of evidence have demonstrated the therapeutic potential of mesenchymal stem cells (MSCs) in regenerative medicine. MSCs not only replenish the lost cells, they also promote the regeneration of impaired tissues by modulating the immune responses. Following the development of 3D cell culture, the enhanced therapeutic efficacy of spheroid-forming MSCs have been identified in several animal disease models by promoting differentiation or trophic factor secretion, as compared to planar-cultured MSCs. Due to the complicated and multifunctional applications in the medical field, MSCs are recently named as medicinal signaling cells. In this review, we summarize the predominant differences of cell-environment interactions for the MSC spheroids formed by chitosan-based substrates and other scaffold-free approaches. Furthermore, several important physical and chemical factors affecting cell behaviors in the cell spheroids are discussed. Currently, the understanding of MSCs spheroid interactions is continuously expanding. Overall, this article aims to review the broad advantages and perspectives of MSC spheroids in regenerative medicine and in future healthcare. Three-Dimensional (3D) Cell Culture SystemsCell-cell and cell-environment interactions cooperatively determine the physiological phenomena in vivo. Many cell growth mechanisms and signal transduction pathways occurring in cells have been unveiled by the traditional two-dimensional (2D) culture system. Plastic-based culture materials remain the predominant cell culture platforms to date, yet some concerns have been raised with the accumulating experimental evidence.A major drawback of cell culture on 2D plastic plates is the planar cell-cell interaction that has the insufficient and inappropriate cell-environment crosstalk. In vivo, cells are grown and stacked within a three-dimensional (3D) space, and extracellular matrix (ECM) secreted by cells constitutes the supporting framework to further organize tissues and organs. Therefore, some important cell-cell and cell-environment interactions may be ignored in a planar culture platform. Primary cells, cancer cells, and stem cells have been cultured in a 3D environment, and many interesting findings have been uncovered. In general, the definition of 3D cell culture systems is that cells are cultured on/in the 3D scaffolds composed by natural or synthetic materials, or they are organized into cellular spheroids. With the continuing development of 3D cell culture, it is now generally agreed that the cell physiology and cell behavior are dramatically distinct for cells cultured within a 2D or 3D environment [1][2][3].Cancer cells are the most common cell types that have been investigated by 3D culture systems, and obvious change of gene expression as well as intracellular signaling in these 3D-cultured cancer cells have been ...

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The Role of Physical Stimuli on Calcium Channels in Chondrogenic Differentiation of Mesenchymal Stem Cells

Cited by 56 publications

References 62 publications

Electrical stimulation in bone tissue engineering treatments

Electrical stimulation in bone tissue engineering treatments

New Insights on Mechanical Stimulation of Mesenchymal Stem Cells for Cartilage Regeneration

Cellular Spheroids of Mesenchymal Stem Cells and Their Perspectives in Future Healthcare

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