The role of PI3K signaling pathway and its associated genes in papillary thyroid cancer

Amjad, Elham; Asnaashari, Solmaz; Sokouti, Babak

doi:10.1186/s43046-021-00068-2

Cited by 4 publications

(2 citation statements)

References 44 publications

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“…3 A– 3 H). The lncRNAs and mRNAs significantly enriched in the PI3K-Akt signaling pathway in KEGG analysis included LINC02082, LOC105369812, and LOC105375170, and EPHA2, LAMB3, and LPAR5, respectively, which play key roles in PTC pathogenesis, [ 13 ] were selected as candidate lncRNAs.…”

Section: Resultsmentioning

confidence: 99%

Significance of LINC02082 and LOC105369812 in differentiating papillary thyroid cancer from benign nodules

Xu,

Liu,

et al. 2023

Medicine

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Objective: To explore the significance of LINC02082 and LOC105369812 in the differential diagnosis of papillary thyroid carcinoma (PTC) and benign nodules. Methods: Cancer tissues and benign nodules from 8 patients were sequenced and constructed using high-throughput sequencing. Differentially expressed mRNAs (DEmRNAs) and lncRNAs (DElncRNAs) with significant differences were screened. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed on the mRNAs co-expressed by DEmRNAs and DElncRNAs. LncRNAs with significant differences, good consistency, and enrichment in the PI3K-AKt signaling pathway were selected as candidate lncRNAs, and the target lncRNAs were screened by correlation analysis. Target lncRNAs and co-expressed mRNAs enriched in the PI3K-AKt signaling pathway and microRNAs (miRNAs) interacting with each other were used to construct a competing endogenous RNA (ceRNA) network. Finally, the PTC-related gene set (GSE33630) was downloaded from the GEO database and the expression of the genes obtained by sequencing was compared. Differential expression was verified using quantitative real-time PCR (qRT-PCR). Finally, the receiver operating characteristic (ROC) curve was used to evaluate the value of the target lncRNAs in diagnosis, when used alone or in combination. Results: A total of 1113 differential RNAs (DE RNAs) were identified, of which 338 were DElncRNAs and 775 were DEmRNAs. Three lncRNAs enriched in the PI3K-AKt signaling pathway, LINC02082, LOC105369812, and LOC105375170, were used as candidate lncRNAs. After correlation analysis with known biomarkers, LINC02082 and LOC105369812 were selected as the target lncRNAs. The qRT-PCR results showed that the target lncRNAs were significantly different among the 3 tissues. The ROC curve showed that LOC105369812 could be used to differentiate PTC from benign thyroid nodules, whereas LINC02082 and its combination had lower predictive value. Conclusions: LOC105369812 is valuable for differentiating benign from malignant thyroid nodules, whereas LINC02082 has lower diagnostic value.

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Section: Resultsmentioning

confidence: 99%

Significance of LINC02082 and LOC105369812 in differentiating papillary thyroid cancer from benign nodules

Xu,

Liu,

et al. 2023

Medicine

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“…The PI3k-Akt signaling pathway is a well-known intracellular apoptotic pathway which is closely associated with the occurrence of PTC. 68 AKT1 is a key regulator in cell motility and the apoptotic process, the activation of which promotes metastasis in thyroid cancer. 69 Four out of 9 core targets identified in this study, namely VEGFA, AKT1, EGFR, and HSP90AA1, are in the PI3k-Akt signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Exploring Molecular Targets and Mechanisms of Apigenin in the Treatment of Papillary Thyroid Carcinoma Based on Network Pharmacology and Molecular Docking Analysis

Wang

Jing

et al. 2022

Natural Product Communications

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To explore potential therapeutic targets and underlying mechanisms of apigenin (API) for papillary thyroid carcinoma (PTC) treatment. Methods: In the present study, network pharmacological analysis combined with molecular docking was used to identify API-related therapeutic targets for PTC treatment. Firstly, potential API-interacting and PTC-related targets were obtained from online databases. Furthermore, we constructed drug-target-disease interaction networks followed by functional enrichment analysis, expression patterns and prognostic values of predicted core targets by using bioinformatic tools. Moreover, we performed molecular docking to validate binding activity of API to core pharmacological targets. Results: A total of 110 API-related therapeutic targets were found to be engaged in the treatment of PTC. Moreover, TP53, HSP90AA1, AKT1, EGFR, SRC, VEGFA, ACTB, JUN and ESR1 were identified as core pharmacological targets of API for treating PTC. The enrichment analysis suggested that API played a comprehensive role in regulating the apoptotic process, modulating cell proliferation, and that multiple pathways were involved in API-related PTC treatment. Further results indicated that the PI3k-Akt/p53 signaling pathway was the key signaling pathway. Moreover, JUN was found mostly correlated with survival and molecular docking results demonstrating strong affinities between API and these core targets. Conclusion: Our findings systematically demonstrated API-associated pharmacological targets and mechanisms for treating PTC and demonstrated that API could function against PTC by inducing apoptosis, cell death and immunological reactions. Our research gives valuable insights and a theoretical basis for API-related PTC treatment.

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The role of MAPK, notch and Wnt signaling pathways in papillary thyroid cancer: Evidence from a systematic review and meta-analyzing microarray datasets employing bioinformatics knowledge and literature

Amjad,

Asnaashari,

Jahanban-Esfahlan

et al. 2024

Biochemistry and Biophysics Reports

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The role of PI3K signaling pathway and its associated genes in papillary thyroid cancer

Cited by 4 publications

References 44 publications

Significance of LINC02082 and LOC105369812 in differentiating papillary thyroid cancer from benign nodules

Significance of LINC02082 and LOC105369812 in differentiating papillary thyroid cancer from benign nodules

Exploring Molecular Targets and Mechanisms of Apigenin in the Treatment of Papillary Thyroid Carcinoma Based on Network Pharmacology and Molecular Docking Analysis

The role of MAPK, notch and Wnt signaling pathways in papillary thyroid cancer: Evidence from a systematic review and meta-analyzing microarray datasets employing bioinformatics knowledge and literature

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