The Role of Plasminogen in Fibrinolysis

Wit, C. Dudok de

doi:10.1159/000464817

Cited by 5 publications

(6 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We have confirmed that plasma and serum do not differ significantly in their plasminogen content (3,4). In addition we have found that the formation of an in vitro thrombus is not associated with loss of plasminogen from the blood from which it is made, and direct measurement of the plasminogen content of thrombi suggests that serum trapped in its interstices may account for the small amount of plasminogen found.…”

Section: Discussionsupporting

confidence: 71%

The Plasminogen Content of Thrombi

Ogston¹,

Cm²,

Hw³

1966

Thromb Haemost

View full text Add to dashboard Cite

Summary1. The plasminogen content of serum and plasma does not differ. The serum remaining after the production of an in vitro artificial thrombus has a plasminogen content similar to the original plasma. Direct measurement of the plasminogen content of artificial thrombi confirms that little plasminogen is adsorbed onto fibrin during the process of clotting.2. The lysis of pre-formed artificial thrombi by urokinase is substantially increased in the presence of plasma. This is not due to free plasmin in the medium surrounding the thrombus.3. Partially purified plasminogen is adsorbed onto artificial thrombi endowing them with the capacity to lyse on the addition of urokinase. In contrast plasminogen from plasma or serum is not adsorbed onto thrombi.4. We suggest that the lysis of a thrombus results from diffusion of circulating plasminogen into the thrombus and its interaction with activator on the surface of the fibrin fibrils with consequent plasmin formation.

show abstract

Section: Discussionsupporting

confidence: 71%

The Plasminogen Content of Thrombi

Ogston¹,

Cm²,

Hw³

1966

Thromb Haemost

View full text Add to dashboard Cite

show abstract

“…This increase in lysis was accentuated if the plasma plasminogen was also high, but this cannot be due only to the plasminogen content of the plasma surrounding the thrombus since it occurred when the thrombi were incubated in urokinase alone. It has been suggested that plasminogen is adsorbed onto fibrin during clotting in an amount sufficient for subsequent lysis , but there is evidence against this hypothesis (Dudok de Wit 1962. We consider that the greater lysis of large thrombi is secondary to a relatively greater amount of serum, containing plasminogen, trapped in their fibrin interstices.…”

Section: Discussionmentioning

confidence: 71%

Factors Influencing the Lysis of Artificial Thrombi by Urokinase

Ogston

1967

Scandinavian Journal of Haematology

View full text Add to dashboard Cite

The urokinase‐induced lysis of artificial thrombi increased as their fibrin content rose. The plasminogen concentration of the plasma from which they were made also influenced their subsequent lysis. The presence of plasma outside the thrombi contributed to their lysis only when the thrombi were small. No relationship was found between the lysis of artificial thrombi by urokinase and their solubility in 1 per cent monochloroacetic‐acid. The platelet content of the blood from which the thrombi were made did not influence the lysis of their fibrin component.

show abstract

“…Fibrinolysis was measured with the aid of standard clots labelled with 'SII-Fibrinogen according to DUDOK DE WIT (1962).…”

Section: Methodsmentioning

confidence: 99%

“…Congenital haemorrhagic diathesis has been observed in animals, especially in the dog (FIELD et al, 1946;HUTT et al, 1948;GRAHAM et al, 1949;LEWIS et al, 1951;ARCHER and BOWDEN, 1959;MUSTARD et al, 1960 and1962). ROWSELL et al (1960) described the occurrence of hemophilia in the dog and distinguished between hemophilia-A and hemophilia-B.…”

Section: Introductionmentioning

confidence: 99%

Congenital Afibrinogenemia in Goats

Breukink

Hart

Arkel

et al. 2010

Zentralblatt Für Veterinärmedizin Reihe A

View full text Add to dashboard Cite

Summary A case of congenital afibrinogenemia in goats is described. The animal shows severe haemorrhagic diathesis with joint bleedings. The patient's blood was absolutely incoagulable. Immunologically no fibrinogen could be demonstrated in the plasma. The other clotting factors were present in normal concentrations. Fibrinolytic activity was not increased. The bleeding time was prolonged and the platelet adhesiveness on passage through a glass filter was diminished. The radiologic examination revealed the presence of a progressive change in the structure of the bone tissue. Multiple bone cysts developed. Microscopic examination revealed the presence of hyalin globules in the liver cells. Genetic examinations were carried out during six years. Four out of 34 descendents had the same symptoms as the first patient. A low fibrinogen content seems to be an expression of heterozygosity. Because of the severity of the bleeding tendency it was not possible to keep the animals alive for long. The genetic investigation led to the hypothesis that congenital afibrinogenemia in the goat may be considered as an “inborn error of metabolism” and could be due to a point mutation in a control gene. The hereditary pattern of the phenotype (fibrinogen content) seems to be incompletely dominant. Zusammenfassung In der vorliegenden Arbeit wird ein Fall von angeborener Afibrinogenämie bei einer Ziege beschrieben. Das Tier litt unter schwerer hämorrhagischer Diathese mit Blutungen in die Gelenke. Das Blut des Patienten war nicht gerinnungsfähig. Immunologisch konnte kein Fibrinogen im Plasma nachgewiesen werden. Die restlichen Gerinnungsfaktoren waren hingegen in normaler Konzentration vorhanden. Die Fibrinolyse‐Aktivität war nicht erhöht. Die Blutungszeit war verlängert und das Klebevermögen der Blutplättchen beim Passieren eines Glasfilters war vermindert. Die röntgenologische Untersuchung zeigte progressive Veränderungen im Knochengewebe. Es entwickelten sich vielfache Knochenzysten. Mikroskopisch stellte man Hyalintropfen in den Leberzellen fest. Während 6 Jahre wurden genetische Untersuchungen vorgenommen. 4 von 34 Nachkommen des ersten Patienten zeigten dieselben Symptome. Ein niedriger Fibrinogenspiegel scheint ein Anzeichen von Heterozygotie zu sein. Wegen der schweren Form der hämorrhagischen Diathese war es nicht möglich, die Tiere lange am Leben zu halten. Auf Grund der genetischen Untersuchung gelangen die Autoren zu folgender Hypothese: die kongenitale Afibrinogenämie der Ziege ist eine “angeborene Fehlleistung des Stoffwechsels” und könnte ihre Ursache in einer Punktmutation im Kontrollgen haben. Die Vererbung des Phänotyps (Fibrinogengehalt) scheint inkomplett dominant zu sein. Résumé Afibrinogènémie congénitale chez les chèvres Dans le présent travail, on décrit un cas d'afribrinogènémie congénitale chez une chèvre. L'animal souffre d'une diathèse hémorragique grave, avec hémorragies articulaires. Le sang du patient est incoagulable. A l'immunologie, on ne trouve pas de fibrinogène dans le plasma. Par contre, les autres...

show abstract

The Role of Plasminogen in Fibrinolysis

Cited by 5 publications

References 7 publications

The Plasminogen Content of Thrombi

The Plasminogen Content of Thrombi

Factors Influencing the Lysis of Artificial Thrombi by Urokinase

Congenital Afibrinogenemia in Goats

Contact Info

Product

Resources

About