The role of platelet CD154 in the modulation in adaptive immunity

Sprague, Daniel L.; Sowa, Jennifer M.; Elzey, Bennett D.; Ratliff, Timothy L.

doi:10.1007/s12026-007-0074-3

Cited by 45 publications

(29 citation statements)

References 37 publications

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“…36 PLT-to-LC regulations were demonstrated in PLTmediated inflammatory responses, [37][38][39] and in the modulation of lymphocytes. 40,41 Conversely, less attention has been paid to LC-to-PLT modulation. Previous studies were mainly focused on the influence of LC on the downstream immune/defense responses elicited by PLTs, 36 while DC-mediated regulation of PLTs in response to engagement of CD14 ϩ LC was not demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Dendritic cells modulate platelet activity in IVIg-mediated amelioration of ITP in mice

Huang

Sun

Lien³

et al. 2010

Blood

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Intravenous immunoglobulin (IVIg) is an effective treatment against immune thrombocytopenia (ITP). Previous studies suggested that IVIg exerts this ame IntroductionImmune thrombocytopenia (ITP) is a disorder manifested by immune-mediated low platelet (PLT) counts. 1-3 Treatment with high-dose intravenous immunoglobulin (IVIg), a human IgG fraction prepared from pools of plasma of thousands of donors, can rapidly relieve the symptoms of thrombocytopenia. 2,4 The PLT clearance is mainly mediated through IgG Fc receptors (Fc␥R) bearing macrophages in the mononuclear phagocytic system (MPS). 5 In mice, both inhibitory and activating Fc␥Rs, the Fc␥RIIB and Fc␥RIII (encoded by Fcgr2b and Fcgr3, respectively) are important for IVIg-mediated amelioration. [6][7][8] IVIg treatment was shown to induce macrophages expressing Fc␥RIIB, by which it reduced the phagocytic activity of the macrophages. 5,7,8 In addition, Fc␥RIII on CD11c ϩ dendritic cells (DCs) is important for IVIg-mediated amelioration of ITP in a 2-step priming model, in which IVIg could modulate effector leukocytes indirectly through initiator DCs. 5,9 The modulation of PLTs, however, is less characterized. The splenic MPS plays important roles in the phagocytic clearance of PLT during ITP, 1,10,11 suggesting that the severity of ITP is associated with PLT-phagocyte engagements. Thus, we established a flow cytometry-based PLT-splenic CD14 ϩ leukocyte (PLT-CD14 ϩ LC) engagement analysis to investigate the roles of PLTs and phagocytes in IVIg-primed DCs (IVIg-DCs)-mediated modulations. The involvements of P-selectin, Fc␥RIIB, and Fc␥RIII in the amelioration of ITP are discussed. Methods IVIg and miceIVIg was purchased from Bayer (Gamimune N) and CSL Limited Inc. The C57BL/6J mice (males, 7-10 weeks old) were purchased from the National Laboratory Animal Center (NLAC). C57BL/6J mice deficient in Fc␥RIII (B6;129P2-Fcgr3 tm1Sjv /J), Fc␥RIIB (B6;129S4-Fcgr2b tm1Rav /J), and Pselectin (B6;129S2-Selp tm1Hyn /J), were purchased from The Jackson Laboratory. Mice were housed in the Laboratory Animal Center of Tzu Chi University. The research methods were approved by the Animal Care and Use Committee of Tzu-Chi University. Induction and reversal of murine ITPITP was induced and treated as previously described. 7 Mice were intravenously injected with 0.1 mg/kg body weight of anti-PLT monoclonal antibody (mAb, rat anti-mouse integrin ␣ IIb /CD41 Ig, clone MWReg30; BD Biosciences) to induce ITP in all experiments, except for the low-dose treatments of MWReg30 (0.03 mg/kg) described in supplemental Figure 1 (available on the Blood web site; see the Supplemental Materials link at the top of the online article). To analyze PLT counts, whole blood samples (50-100 L) of mice were collected from the retro-orbital venous plexus and mixed with anticoagulant ACD solution (38mM citric acid, 75mM sodium citrate, 100mM dextrose) in Eppendorf tubes. PLT counts were then measured with a hematology analyzer (KX-21N; Sysmex) at various time intervals (0, 2, 4, and 24 hours after anti-CD41...

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Section: Discussionmentioning

confidence: 99%

Dendritic cells modulate platelet activity in IVIg-mediated amelioration of ITP in mice

Huang

Sun

Lien³

et al. 2010

Blood

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“…Moreover, studies that are more recent, demonstrated that platelet-derived vesicles might transport and deliver various molecules among cells (9,143,144). Platelet-derived vesicles also participate in innate (145,146) and adapted (62,147) immunity, in inflammation (148), malignancies (149) and in hematopoietic stem cell engraftment (150). They promote endothelial repair and support angiogenesis (151).…”

Section: Background and Clinical Potentialmentioning

confidence: 99%

Platelet‐derived extracellular vesicles in plateletpheresis concentrates as a quality control approach

et al. 2015

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BACKGROUND Platelet‐derived extracellular vesicles (PL‐EVs) are present in plateletpheresis concentrates (PCs) and may influence the quality of PCs. The aim of the study was to analyze PC‐derived PL‐EVs and to correlate them with standard quality control (QC) variables of PCs and with donor‐specific laboratory variables. STUDY DESIGN AND METHODS PL‐EVs were analyzed by standard as well as advanced high‐sensitivity flow cytometry (FCM) and nanoparticle tracking analysis. A hematology analyzer was applied to the determination of platelet (PLT) count and immature PLT fraction (IPF). Functional capacity of PLTs (CD62P in response to thrombin receptor‐activating peptide 6 activation) was measured by FCM. All in vitro measurements were carried out on Day 0 and on Day 5. Altogether, a total of 42 PC samples, 15 irradiated on Day 0, were investigated. RESULTS Externalization of CD62P, as an indicator of intact PLT function, significantly decreased during in vitro PLT senescence and CD62P expression inversely correlated with increased PL‐EV levels. Interestingly, in fresh PCs a significant correlation was found between PL‐EVs and different hemapheresis instruments, duration of apheresis, and IPF count in peripheral blood of the donor before apheresis. In senescent PCs, the body mass index of donors inversely correlated with the PL‐EV counts. CONCLUSION Loss of PLT function in PCs was associated with increased PL‐EV levels. Shedding of PL‐EVs depends on shear stress influenced by different hemapheresis settings and diverse preanalytical conditions of donors. PL‐EV analysis may stimulate new quality and apheresis strategies for more vital PLTs for transfusion.

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“…Both of them are important and necessary response to bacterial infection and contain antimicrobial peptides that act against a broad range of pathogens (Elzey et al, 2005 andFitzgerald et al, 2006) demonstrated that platelets are an integral part of inflammation and can also be potent effector cells of the innate immune response. The platelet (CD154 molecule) has a vital importance to adaptive immune responses, is expressed by activated platelets and has been implicated in platelet-mediated modulation of innate immunity and inflammatory disease states (Elzey et al, 2005, Sprague et al, 2007 Antimicrobial peptides from white blood cells and platelets exert a rapid, potent and direct antimicrobial effect that contributes to limiting the infection. In addition, both of neutrophil and lymphocyte cells increased after one week, although after two weeks only lymphocyte cells increased.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Pathogenicity of Actinomycetes (Nocardiopsisdassonvillei) spores on male albino rats.

Saad¹,

Ahmed²,

Nabil³

2016

IOSR

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Abstracts:Nocardiopsis dassonvillei was isolated from the indoor air of critical areas of hospitals and its pathogenicity was evaluated in vivo. Male albino rats were injected I.P with Nocardiopsis dassonvillei spores (1×10 7 /rat ) for 2 weeks. Different changes in haematological and biochemical parameters after one and two weeks of actinomycete spores injection were assessed. Nocardiopsis infection caused RBCs count decrease and significantly decline Hb concentration compared to control group, while significant increase in WBCs, Neutrophil, Lymphocytes and Platelets were observed. Hepatotoxicity of Nocardiopsis was also investigated through severe drop in the activity of liver function markers (ALT, AST) after 2 weeks. Moreover, it induced hepatic apoptosis which is detected by caspase 3. It can be concluded that exposure to Nocardiopsis dassonvillei spores may cause haematological , biochemical disorders and heptotoxicity,

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The role of platelet CD154 in the modulation in adaptive immunity

Cited by 45 publications

References 37 publications

Dendritic cells modulate platelet activity in IVIg-mediated amelioration of ITP in mice

Dendritic cells modulate platelet activity in IVIg-mediated amelioration of ITP in mice

Platelet‐derived extracellular vesicles in plateletpheresis concentrates as a quality control approach

In Vivo Pathogenicity of Actinomycetes (Nocardiopsisdassonvillei) spores on male albino rats.

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