The Role of Platelets in Antimicrobial Host Defense

Yeaman, Michael R.

doi:10.1016/b978-0-12-813456-6.00029-1

Cited by 28 publications

(32 citation statements)

References 334 publications

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“…PLTs are small particles of megakaryocytes that originate via a mechanism that is similar to that of macrophages and granulocytes, that is, from myeloid progenitors (27,28). There are 3 types of particles produced by PLTs: a-granules, d-granules, and l-granules (29). These particles contain different substances, including growth factors, blood coagulation factors, and PLT microbicidal proteins, that establish the functional diversity of PLTs.…”

Section: Discussionmentioning

confidence: 99%

Platelets directly regulate DNA damage and division ofStaphylococcus aureus

Zhang

et al. 2018

FASEB j.

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Platelets (PLTs) are classically used in the clinical setting to maintain hemostasis. Recent evidence supports important roles for PLTs in host inflammatory and immune responses, and PLT-rich plasma has been demonstrated to inhibit the growth of bacteria in vitro and in vivo; however, few studies have examined whether PLTs can inhibit bacterial growth directly, and related mechanisms have not been elucidated further. Accordingly, in this study, we evaluated the effects of PLTs on bacterial growth. We washed and purified PLTs from peripheral blood, then confirmed that PLTs significantly inhibited the growth of Staphylococcus aureus when cocultured in vitro. Moreover, PLTs damaged DNA and blocked cell division in S. aureus. During coculture, PLT-derived TGF-β1 was dramatically down-regulated compared with that in PLT culture alone, and the addition of TGF-β1 to the coculture system promoted the inhibition of PLTs on S. aureus. Analysis of a murine S. aureus infection model demonstrated that the depletion of PLTs exacerbated the severity of infection, whereas the transfusion of PLTs alleviated this infection. Our observations demonstrate that PLTs could directly inhibit the growth of S. aureus by damaging DNA and blockage cell division, and that PLT-derived TGF-β1 may play an important role in this machinery.-Xu, J., Yi, J., Zhang, H., Feng, F., Gu, S., Weng, L., Zhang, J., Chen, Y., An, N., Liu, Z., An, Q., Yin, W., Hu, X. Platelets directly regulate DNA damage and division of Staphylococcus aureus.

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Section: Discussionmentioning

confidence: 99%

Platelets directly regulate DNA damage and division ofStaphylococcus aureus

Zhang

et al. 2018

FASEB j.

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“…PRGF® has been shown to have antibacterial effects on Staphylococcus aureus and Staphylococcus epidermidis strains, 13,14 effects that may be mediated by the platelets which have been shown to have many functions in the antimicrobial host defence systems. 29 By contrast, Alvogyl® contains the antiseptic iodoform which has been shown to have an antibacterial effect on several strains of oral bacteria. 30 In the present study the increase in the numbers of patients with halitosis in the Alvogyl® group could reflect a decline in antimicrobial effects of the dressing, but perhaps also may reflect the potential that there was an increased accumulation of oral debris and bacteria due to dressing loss.…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Plasma Rich in Growth Factors for the Treatment of Alveolar Osteitis: A Randomized Controlled Trial

King

Cerajewska

Locke

et al. 2018

Journal of Oral and Maxillofacial Surgery

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“…[55][56][57] Some of the antimicrobial agents found include the antimicrobial peptides released by platelets, the platelet microbiocidal proteins, the fibrinopeptides A and B, the platelet basic proteins, the thymosin ß-4, the platelet factor IV, the connective tissue-activating peptide III, and the RANTES (regulated upon activation normal T-Cell expressed and secreted). 58,59 The sharp reduction in the bacterial adhesion at AE 1 Ca 1 Plasma surfaces may thus be highly associated to this platelet activation process.…”

Section: Discussionmentioning

confidence: 99%

Balancing microbial and mammalian cell functions on calcium ion‐modified implant surfaces

et al. 2017

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Implant integration is a complex process mediated by the interaction of the implant surface with the surrounding ions, proteins, bacteria, and tissue cells. Although most implants achieve long-term bone-tissue integration, preventing pervasive implant-centered infections demands further advances, particularly in surfaces design. In this work, we analyzed classical microrough implant surfaces (only acid etched, AE; sandblasted then acid etching, SB 1 AE) and a new calcium-ion-modified implant surface (AE 1 Ca) in terms of soft-and hard-tissue integration, bacterial adhesion, and biofilm formation. We cultured on the surfaces primary oral cells from gingiva and alveolar bone, and three representative bacterial strains of the oral cavity, emulating oral conditions of natural saliva and blood plasma. With respect to gingiva and bone cells and in the presence of platelets and plasma proteins, AE 1 Ca surfaces yielded in average 86% higher adhesion, 44% more proliferation, and triggered 246% more synthesis of extracellular matrix biomolecules than AE-unmodified controls. Concomitantly, AE 1 Ca surfaces regardless of conditioning with saliva and/or blood plasma showed significantly less bacterial adhesion (67% reduction in average) and biofilm formation (40% reduction in average) than unmodified surfaces. These results highlight the importance of a calcium-rich hydrated interface to favor mammalian cell functions over microbial colonization at implant surfaces.

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The Role of Platelets in Antimicrobial Host Defense

Cited by 28 publications

References 334 publications

Platelets directly regulate DNA damage and division ofStaphylococcus aureus

Platelets directly regulate DNA damage and division ofStaphylococcus aureus

The Efficacy of Plasma Rich in Growth Factors for the Treatment of Alveolar Osteitis: A Randomized Controlled Trial

Balancing microbial and mammalian cell functions on calcium ion‐modified implant surfaces

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