The Role of PME2 and PME3 in Arabidopsis Stomatal Development and Morphology †

Tsakali, Amalia; Asitzoglou, Ioannis-Christos; Basdeki, Vassiliki; Podia, Varvara; Adamakis, Ioannis-Dimosthenis S.; Giannoutsou, Eleni; Haralampidis, Kosmas

doi:10.3390/iecps2021-12010

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…The expression of AtPME2 was improved during lateral root emergence and hypocotyl elongation [27]. Furthermore, both AtPME2 and AtPME3 participated in stomatal development, and regulated guard cell movement [28]. AtPME35 was specifically expressed in Arabidopsis in the basal part of the inflorescence stem, and was involved in regulating the stem's mechanical strength [29].…”

Section: Discussionmentioning

confidence: 99%

“…For example, AtPME41 in Arabidopsis [25] and nine CsPME genes in navel orange were induced by low temperature treatment [20]. In contrast, AtPME34 was induced by heat stress and ABA; additionally, it was highly expressed in guard cells [26], whilst AtPME2 was associated with lateral root emergence and hypocotyl elongation, stomatal development and guard cell movement [27,28]. Moreover, AtPME35 was involved in regulating stem mechanical strength [29], AtPME6 was increased during embryo development [30] and AtPME31 was induced by salt stress [31].…”

Section: Introductionmentioning

confidence: 99%

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Integrated Characterization of Cassava (Manihot esculenta) Pectin Methylesterase (MePME) Genes to Filter Candidate Gene Responses to Multiple Abiotic Stresses

Wang

Zhou

et al. 2023

Plants

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Plant pectin methylesterases (PMEs) play crucial roles in regulating cell wall modification and response to various stresses. Members of the PME family have been found in several crops, but there is a lack of research into their presence in cassava (Manihot esculent), which is an important crop for world food security. In this research, 89 MePME genes were identified in cassava that were separated into two types (type-Ⅰ and type-Ⅱ) according to the existence or absence of a pro-region (PMEI domain). The MePME gene members were unevenly located on 17 chromosomes, with 19 gene pairs being identified that most likely arose via duplication events. The MePMEs could be divided into ten sub-groups in type-Ⅰ and five sub-groups in type-Ⅱ. The motif analysis revealed 11 conserved motifs in type-Ⅰ and 8 in type-Ⅱ MePMEs. The number of introns in the CDS region of type-Ⅰ MePMEs ranged between one and two, and the number of introns in type-Ⅱ MePMEs ranged between one and nine. There were 21 type-Ⅰ and 31 type-Ⅱ MePMEs that contained signal peptides. Most of the type-Ⅰ MePMEs had two conserved “RK/RLL” and one “FPSWVS” domain between the pro-region and the PME domain. Multiple stress-, hormone- and tissue-specific-related cis-acting regulatory elements were identified in the promoter regions of MePME genes. A total of five co-expressed genes (MePME1, MePME2, MePME27, MePME65 and MePME82) were filtered from different abiotic stresses via the use of UpSet Venn diagrams. The gene expression pattern analysis revealed that the expression of MePME1 was positively correlated with the degree of cassava postharvest physiological deterioration (PPD). The expression of this gene was also significantly upregulated by 7% PEG and 14 °C low-temperature stress, but slightly downregulated by ABA treatment. The tissue-specific expression analysis revealed that MePME1 and MePME65 generally displayed higher expression levels in most tissues than the other co-expressed genes. In this study, we obtain an in-depth understanding of the cassava PME gene family, suggesting that MePME1 could be a candidate gene associated with multiple abiotic tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Integrated Characterization of Cassava (Manihot esculenta) Pectin Methylesterase (MePME) Genes to Filter Candidate Gene Responses to Multiple Abiotic Stresses

Wang

Zhou

et al. 2023

Plants

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“…Various environmental parameters, such as light intensity, atmospheric CO 2 concentration, water availability [ 31 ], or even immersion of the plants in 1–5% sucrose solution [ 32 ], affect stomatal density. Thus, stomatal aggregations are affected by both environmental and genetic parameters [ 33 , 34 , 35 , 36 ]. Stomatal clustering has also been observed in Begonia , where it has been considered an adaptation for growth in arid environments [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Stomata in Close Contact: The Case of Pancratium maritimum L. (Amaryllidaceae)

Saridis

Georgiadou

Shtein³

et al. 2022

Plants

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A special feature found in Amaryllidaceae is that some guard cells of the neighboring stomata form a “connection strand” between their dorsal cell walls. In the present work, this strand was studied in terms of both its composition and its effect on the morphology and function of the stomata in Pancratium maritimum L. leaves. The structure of stomata and their connection strand were studied by light and transmission electron microscopy. FM 4–64 and aniline blue staining and application of tannic acid were performed to detect cell membranes, callose, and pectins, respectively. A plasmolysis experiment was also performed. The composition of the connection strand was analyzed by fluorescence microscopy after immunostaining with several cell-wall-related antibodies, while pectinase treatment was applied to confirm the presence of pectins in the connection strand. To examine the effect of this connection on stomatal function, several morphological characteristics (width, length, size, pore aperture, stomatal distance, and cell size of the intermediate pavement cell) were studied. It is suggested that the connecting strand consists of cell wall material laid through the middle of the intermediate pavement cell adjoining the two stomata. These cell wall strands are mainly comprised of pectins, and crystalline cellulose and extensins were also present. Connected stomata do not open like the single stomata do, indicating that the connection strand could also affect stomatal function. This trait is common to other Amaryllidaceae representatives.

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Genome-Wide Characterization of Class III Peroxidases and Their Expression Profile During Mycorrhizal Symbiosis and Phosphorus Deprivation in Lettuce (Lactuca sativa L.)

Simoni,

Castellacci,

Usai

et al. 2024

Horticulturae

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Lettuce cultivation requires high fertilizer inputs, which impact the environment and costs. Arbuscular mycorrhizal symbiosis (AMS) can reduce fertilizer use, enhance plant nutrition (especially phosphorus), and promote healthier plants. Class III peroxidases (PRXs) play crucial roles in various physiological processes and stress responses. However, their role in AMS and phosphorous (P) deficiency is still unclear. Our study identified 91 PRX genes in the lettuce genome (LsPRXs) and clustered them into eight subfamilies based on phylogenetic relationships. Evolutionary analysis indicated that tandem duplication was the main driver for LsPRX gene family expansion. Synteny analysis showed orthologous relationships of the PRX gene family between lettuce and potato, Arabidopsis, and maize, identifying 39, 28, and 3 shared PRXs, respectively. Transcriptomic data revealed that most LsPRX genes were more expressed in roots than in leaves and differentially expressed LsPRXs were found in response to AMS and P supply. Notably, 15% of LsPRX genes were differentially expressed in roots during mycorrhization. Gene expression network analysis highly correlated five LsPRXs (LsPRX17, LsPRX23, LsPRX24, LsPRX64, and LsPRX79) with genes involved in cell wall remodeling and reorganization during mycorrhization. Our results provide insights into the evolutionary history and functional roles of PRX genes in lettuce and identify candidate gene targets that may enhance the bio-stimulant effects of AMS and help to cope with P deficiency.

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The Role of PME2 and PME3 in Arabidopsis Stomatal Development and Morphology †

Cited by 3 publications

References 32 publications

Integrated Characterization of Cassava (Manihot esculenta) Pectin Methylesterase (MePME) Genes to Filter Candidate Gene Responses to Multiple Abiotic Stresses

Integrated Characterization of Cassava (Manihot esculenta) Pectin Methylesterase (MePME) Genes to Filter Candidate Gene Responses to Multiple Abiotic Stresses

Stomata in Close Contact: The Case of Pancratium maritimum L. (Amaryllidaceae)

Genome-Wide Characterization of Class III Peroxidases and Their Expression Profile During Mycorrhizal Symbiosis and Phosphorus Deprivation in Lettuce (Lactuca sativa L.)

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