The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials

Sun, Shao‐Ni; Cao, Xuefei; Sun, Run‐Cang

doi:10.1016/j.biortech.2015.08.061

Cited by 824 publications

(442 citation statements)

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“…After the reaction is completed, because the separated material in the reaction are in the same space, the material dissolved in the biomass may be condensed again in the cellulose and hemicellulose; therefore, a flowthrough process is required. However, the concentrated acid pretreatments likely lead to severe cellulose degradation, high inhibitor concentrations, and serious equipment corrosion (Alvira et al 2010;Sun et al 2016). To avoid degrading cellulose/hemicellulose, thus forming inhibitors, a flow-through reactor for pretreatment of cellulosic biomass at high temperatures and pressure (300 psig) is required.…”

Section: Introductionmentioning

confidence: 99%

Two-Stage Flow-Through Pretreatment of Helianthus tuberosus Residue for Enzymatic Production of Fermentable Sugar by Alkaline and Acidic Solutions

Park¹,

Yoon

Kim

et al. 2017

BioResources

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A response surface methodology (RSM) tool with the Box-Behnken design was used to determine the optimum pretreatment conditions of Helianthus tuberosus residue for the enzymatic production of fermentable sugar with aqueous ammonia and sulfuric acid solutions, for various parameters such as pretreatment solution concentration, temperature, and reaction time. The pretreatment of biomass was performed using these optimized parameters in aqueous ammonia and sulfuric acid solution, followed by hot water, under the same conditions. The process was then performed by changing the sequence.

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Section: Introductionmentioning

confidence: 99%

Two-Stage Flow-Through Pretreatment of Helianthus tuberosus Residue for Enzymatic Production of Fermentable Sugar by Alkaline and Acidic Solutions

Park¹,

Yoon

Kim

et al. 2017

BioResources

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“…Irradiação por raios gama, feixe de elétrons e micro-ondas também podem ser utilizados para tratar o material lignocelulósico. Essas técnicas são, geralmente, combinadas a outras formas de pré-tratamento, já que não possuem a capacidade de remover hemicelulose (SUN et al, 2016).…”

Section: Pré-tratamento Do Materials Lignocelulósicounclassified

“…Há diversos tratamentos químicos que utilizam ácidos, bases, solventes orgânicos, líquidos iônicos e ozônio (ALVIRA; BALLESTEROS; NEGRO, 2010). O pré-tratamento com solução alcalina é o método químico mais utilizado para remover lignina e hemicelulose da biomassa (SUN et al, 2016). Na hemicelulose, o álcali atua removendo grupos acetil e através da substituição de vários ácidos urônicos, aumentando a acessibilidade do material.…”

Section: Pré-tratamento Do Materials Lignocelulósicounclassified

“…Dentre os processos físico-químicos, o de explosão a vapor é o mais comumente utilizado para pré-tratar a biomassa lignocelulósica (SUN et al, 2016). Nesse método a lignocelulose é submetida a alta pressão (0,7 a 4,8 MPa) e temperatura, (160 e 240 o C) durante alguns segundos ou minutos (CANILHA et al, 2012).…”

Section: Pré-tratamento Do Materials Lignocelulósicounclassified

“…Fungos como, por exemplo, Phanerochaete crysosporium, Ceriporia lacerata, Cyathus stercoletus, Ceriporiopsis subvermispora, Pycnoporus cinnabarinus e Pleurotus ostreatus vem sendo utilizados no pré-tratamento de diversos tipos de biomassa (SUN et al, 2016). Esse pré-tratamento não utiliza agentes químicos que podem atuar como inibidores o durante o processo e requer baixa quantidade de energia para ocorrer, pois se trata de um processo biológico.…”

Section: Pré-tratamento Do Materials Lignocelulósicounclassified

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Seleção de fungos filamentosos produtores de hidrolases e pré-otimização das condições de cultivo

Ascencio¹

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The selection of filamentous fungi that produce hydrolases and the preoptimization of their growth conditionsThe increasing demand for energy and the necessity to promote sustainable development has focused on renewable fuels. Within the available biofuels, 2G or second generation, has the aim of releasing the energy contained in plant biomass. Many microorganisms are able to secret enzymes capable of breaking down lignocellulose, the main component of plant cell walls, into fermentable sugars. The objectives of this project was to select filamentous fungi that produce enzymes that degrade biomass and optimize the growth conditions for them. Four isolates and two standards strains were tested for the production of cellulases and identified by ITS (Internal Transcribed Spacer) sequencing. After initial characterization, three strains were selected and tested in different sources of nitrogen and carbon, and buffering agents for optimal growth. The ideal conditions were chosen based on the criteria of enzymatic activity, total proteins released and pH. After the ideal condition for each strain were established, cell free extracts from each culture were lyophilized and the total protein content determined. This extract was tested for FPA (Filter Paper Activity), β-glucosidase and xylanase activity and the hydrolysis assays were carried out using pretreated biomass. The selected strains were identified as Phanerochaete chrysosporium (F88), Aspergillus brasiliensis (F811) and the standard strain Trichoderma reesei QM9414. The best combination of nitrogen sources was the ratio 4:3:3; 5:3:2 and 6:3:1 (ammonium sulfate, urea and yeast extract) for F88, F811 and T. reesei, respectively. Citric acid was selected as buffering agent for F88 and T. reesei, and PIPES for F811. Steam exploded bagasse, as a carbon source, was the best to induce the cellulase and xylanase production for F88 e F811, while, Solka-floc ® was better for T. reesei. The activities of β-glucosidase and xylanase were higher for F811 than for T. reesei. However, for FPA, T. reesei showed the best yield. In the hydrolysis assays, conversions of glucan and xylan were similar for both strains, even though there was an accumulation of celobiose in the T. reesei's assay. Comparing the data obteined for both strains, a wild type and an industrial reference, F811 showed as a promising strain to produce hydrolytic enzymes and as consequence, break down biomass into fermentable sugars.

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Lignocellulose Feedstock Availability, Types of Feedstocks, and New Designer Crops

Bustos¹,

Trujillo²,

Martinez³

et al. 2022

Lignocellulose Bioconversion Through White Biotechnology

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The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials

Cited by 824 publications

References 78 publications

Two-Stage Flow-Through Pretreatment of Helianthus tuberosus Residue for Enzymatic Production of Fermentable Sugar by Alkaline and Acidic Solutions

Two-Stage Flow-Through Pretreatment of Helianthus tuberosus Residue for Enzymatic Production of Fermentable Sugar by Alkaline and Acidic Solutions

Seleção de fungos filamentosos produtores de hidrolases e pré-otimização das condições de cultivo

Lignocellulose Feedstock Availability, Types of Feedstocks, and New Designer Crops

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