The Role of Primary Cilia in Thyroid Cancer: From Basic Research to Clinical Applications

Ma, Chengxu; Ma, Xiaoni; Li, Yingdong; Fu, Songbo

doi:10.3389/fendo.2021.685228

Cited by 7 publications

(13 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Like the medulloblast and basal cell carcinoma, PC was required for or to inhibit tumor formation, depending on the initiating oncogenic event [ 28 , 29 ]. Other researchers indicate that PC was reduced in multiple tumors, restoring PC might be a tumor therapeutic strategy [ 18 ]. Therefore, PC plays a complex role in promoting and preventing tumorigenesis [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…PC has widely been known as a tumour suppressor organelle [ 45 ]. Recent research hypothesizes restoring cilia may be a promising therapeutic strategy in cancers [ 18 ]. There are some pre-clinical examples of PC restoration as a therapeutic target, such as PC restoration by HDAC inhibitors in cholangiocarcinoma decrease cholangiocarcinoma cell growth [ 21 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

“…Through knocking down AURKA, we observed that deficient PC caused by STIL overexpression was restored, suggesting that STIL could influence PC formation through regulating AURKA. Recent research hypothesizes that restoring PC may be a promising therapeutic strategy in cancers [ 18 ]. We found that co-silencing of STIL and AURKA maximizes the restoration of PC in BLCA cells, suggesting these two genes have a synergistic effect on PC regulation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

STIL/AURKA axis promotes cell proliferation by influencing primary cilia formation in bladder cancer

et al. 2023

J Transl Med

View full text Add to dashboard Cite

Background The primary cilia (PC) is a microtubule-based and nonmotile organelle which protrudes from the surface of almost all mammalian cells. At present, PC has been found to be a deficiency or loss in multiple cancers. Restoring PC could be a novel targeting therapy strategy. Our research showed that PC was reduced in human bladder cancer (BLCA) cells, and PC deficiency promotes cell proliferation. However, the concrete mechanisms remain unknown. SCL/TAL1 interrupting locus (STIL), a PC-related protein, was screened in our previous study and could influence the cell cycle by regulating PC in tumor cells. In this study, we aimed to elucidate the function of STIL for PC to explore the underlying mechanism of PC in BLCA. Methods Public database analysis, western blot, and enzyme-linked immunosorbent assay (ELISA) were used to screen genes and explore gene expression alteration. Immunofluorescence and western blot were utilized to investigate PC. Wound healing assay, clone formation assay, and CCK-8 assay were used to explore cell migration, growth, and proliferation. The co-immunoprecipitation and western blot were employed to reveal the interaction of STIL and AURKA. Results We found that high STIL expression is correlated with poor outcomes of BLCA patients. Further analysis revealed that STIL overexpression could inhibit PC formation, activate SHH signaling pathways, and promote cell proliferation. In contrast, STIL-knockdown could promote PC formation, inactivate SHH signaling, and inhibit cell proliferation. Furthermore, we found that the regulatory functions of STIL for PC depend on AURKA. STIL could influence proteasome activity and maintain AURKA stabilization. AURKA-knockdown could reverse PC deficiency caused by STIL overexpression for PC in BLCA cells. We observed that co-knockdown in STIL and AURKA significantly enhanced PC assembly. Conclusion In summary, our result provides a potential therapy target for BLCA based on the restoration of PC.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

STIL/AURKA axis promotes cell proliferation by influencing primary cilia formation in bladder cancer

et al. 2023

J Transl Med

View full text Add to dashboard Cite

show abstract

“…Specific lipids and receptors on PC enable them to sense various extracellular chemical and mechanical signals and transmit them to the intracellular level, invoking responses that regulate varying cellular developmental and physiological processes ( 1 , 5 ). The PC structure consists of a basal body, a transition zone, and an axoneme ( 6 ). The basal body defines cell polarity and initiates ciliogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…Studies show that thyroid illnesses emerge due to oncogenic mutations and abnormal changes in some intracellular downstream signaling pathways and cytokines ( 21 – 23 ). Previous research revealed that as a key mediator, PC influences the pathogenesis of thyroid abnormalities in function and structure ( 6 ). However, there are no inductive studies that describe the systematic role of PC in thyroid diseases.…”

Section: Introductionmentioning

confidence: 99%

The role of primary cilia in thyroid diseases

Tian,

Li,

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

Primary cilia (PC) are non-motile and microtube-based organelles protruding from the surface of almost all thyroid follicle cells. They maintain homeostasis in thyrocytes and loss of PC can result in diverse thyroid diseases. The dysfunction of structure and function of PC are found in many patients with common thyroid diseases. The alterations are associated with the cause, development, and recovery of the diseases and are regulated by PC-mediated signals. Restoring normal PC structure and function in thyrocytes is a promising therapeutic strategy to treat thyroid diseases. This review explores the function of PC in normal thyroid glands. It summarizes the pathology caused by PC alterations in thyroid cancer (TC), autoimmune thyroid diseases (AITD), hypothyroidism, and thyroid nodules (TN) to provide comprehensive references for further study.

show abstract

Primary cilia: Structure, dynamics, and roles in cancer cells and tumor microenvironment

Guan

Zhang

et al. 2023

Journal Cellular Physiology

View full text Add to dashboard Cite

Despite the initiation of tumor arises from tumorigenic transformation signaling in cancer cells, cancer cell survival, invasion, and metastasis also require a dynamic and reciprocal association with extracellular signaling from tumor microenvironment (TME). Primary cilia are the antenna‐like structure that mediate signaling sensation and transduction in different tissues and cells. Recent studies have started to uncover that the heterogeneous ciliation in cancer cells and cells from the TME in tumor growth impels asymmetric paracellular signaling in the TME, indicating the essential functions of primary cilia in homeostasis maintenance of both cancer cells and the TME. In this review, we discussed recent advances in the structure and assembly of primary cilia, and the role of primary cilia in tumor and TME formation, as well as the therapeutic potentials that target ciliary dynamics and signaling from the cells in different tumors and the TME.

show abstract

The Role of Primary Cilia in Thyroid Cancer: From Basic Research to Clinical Applications

Cited by 7 publications

References 48 publications

STIL/AURKA axis promotes cell proliferation by influencing primary cilia formation in bladder cancer

STIL/AURKA axis promotes cell proliferation by influencing primary cilia formation in bladder cancer

The role of primary cilia in thyroid diseases

Primary cilia: Structure, dynamics, and roles in cancer cells and tumor microenvironment

Contact Info

Product

Resources

About