The role of probiotic in the reduction of the colonization of Campylobacter jejuni in broiler chickens

Abstract

Aim of study is to provide efficacy of probiotic to reduce colonization of Campylobacter jejuni in broiler chickens. A total of 150 samples from broiler collected at Qena Province. All samples subjected to bacteriological investigation for C. jejuni and purified colonies identified biochemically. Molecular identification done for isolated strains. Multispecies probiotic product (Durvet) tested in vitro experiment for its inhibitory effects against growth of C. jejuni strains. Seven-day-old Hubbard broiler chicks used in vivo experiment; 120 Chicks classified into six groups; each group 20 chicks; Group (1) negative control, Gp2 infected with C.jejuni, Gp3 treated with probiotic, Gp4) treated with probiotic and infected with C.jejuni, Gp5 feed with supplement and Gp6 feed supplement and infected with C.jejuni. Chicks weighed; performance parameters weekly followed during experiment. (21.3%) C. jejuni identified from chicken by bacteriological examination and (23.3%) positive by molecular. The bacteriological analysis of swabs at end of experiment detected Gp4 and 6 had a pathogen colonization count below 2 log CFU/g, but infected group (Gp2) 7.2 log CFU/g. Weight of birds at 14 d and 28 d increased in Gp1,3,4,5 and 6 but slightly increased in Gp2. The performance was good at 7d in all groups, at 14d and 28d gp1,3,5 and 6 are good and active but depressed, lazy and rough feather in Gp2. In conclusion, the probiotics properties have been antimicrobial activities against C. jejuni.