The Role of Promoter-Associated Histone Acetylation of Haem Oxygenase-1 (HO-1) and Giberellic Acid-Stimulated Like-1 (GSL-1) Genes in Heat-Induced Lateral Root Primordium Inhibition in Maize

Abstract

In plants, lateral roots play a crucial role in the uptake of water and nutrients. Several genes such as Zea mays Haem Oxygenase-1 (ZmHO-1) and Giberellic Acid-Stimulated Like-1 (ZmGSL-1) have been found to be involved in lateral root development. In the present investigation, we observed that heat treatment might be involved in the inhibition of lateral root primordium (LRP) formation in maize, accompanied by an increase in global acetylation levels of histone 3 lysine residue 9 (H3K9) and histone 4 lysine residue 5 (H4K5), suggesting that histone modification was related to LRP inhibition. However, Trichostatin A (TSA), an inhibitor of histone deacetylases (HDACs), apparently did not inhibit the LRP formation, revealing that global hyperacetylation might not be the determining factor in the LRP inhibition induced by heat stress. Furthermore, expression of genes related to lateral root development in maize, ZmHO-1 and ZmGSL-1, was down-regulated and the acetylation levels in the promoter region of these two genes were decreased under heat stress, suggesting that promoter-associated histone acetylation might be associated with the expression of ZmHO-1 and ZmGSL-1 genes which were found to be involved in the heat-induced LRP inhibition in maize.

Sugar transporter proteins in Capsicum: identification, characterization, evolution and expression patterns

“…The plant growth and development are seriously affected by abiotic stress [44][45][46], including high temperature and low temperature [47][48][49][50][51][52][53][54][55][56][57]. In the present study, based on available genome-wide RNA-seq data, we analyzed the expression profile of pepper STP genes under cold and high temperature stress treatments.…”

Histone Deacetylase Inhibitor SAHA Improves High Salinity Tolerance Associated with Hyperacetylation-Enhancing Expression of Ion Homeostasis-Related Genes in Cotton

“…The global change of histone acetylation can also lead to the alternation of chromatin structure [ 25 ]. To further confirm the above results ( Figure 5 D), we used chromatin accessibility real-time PCR (CHART-PCR) to analyze sensitivity of the promoter regions of the ion homeostasis-related genes to micrococcal nuclease (MNase).…”

“…In recent years, exogenous application of small molecules such as abscisic acid, melatonin, and glycinebetaine represent a promising way to increase salt tolerance in plants [ 22 , 23 , 24 ]. HDAC inhibitors (HDACis), a class of natural and synthetic chemical compounds, can suppress the activity of HDACs and increase histone acetylation level, leading to chromatin decondensation and transcriptional activation [ 25 , 26 ]. HDACis have long been regarded as a class of agents for cancer therapy.…”

Redox Components: Key Regulators of Epigenetic Modifications in Plants

“…Acetylated histones can also recruit other proteins, which regulate chromatin structure [38,39]. Generally, hyperacetylation of histones favors transcriptional activation whereas hypoacetylation of histones causes gene repression [22,37,40]. The levels of histone acetylation are regulated by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) [1,41].…”