The Role of Proteases in Determining Stomatal Development and Tuning Pore Aperture: A Review

Fanourakis, Dimitrios; Nikoloudakis, Nikolaos; Pappi, Polyxeni; Markakis, Emmanouil A.; Doupis, Georgios; Charova, Spyridoula N.; Delis, Costas; Tsaniklidis, Georgios

doi:10.3390/plants9030340

Cited by 22 publications

(16 citation statements)

References 84 publications

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“…Plant peptides have emerged as key regulators of stress responses and tolerance [ 56 , 57 ], with a subtilisin-like protease, phytaspase 2, generating the peptide hormone phytosulfokine that regulates drought-induced flower drop in tomato plants [ 28 ]. The phytaspase 2 coding gene, Solyc04g078740, segregating for an SNP in the RIL population, was found as a candidate gene (and at the QTL peak) for both FlN_C_4 and FlN_WD_4.1 ( Table 2 and Table S5 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Root-to-Shoot Signaling and Rootstock-Mediated Tolerance to Water Deficit in Tomato

Asíns

Albacete

Martínez‐Andújar

et al. 2020

Genes

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Developing drought-tolerant crops is an important strategy to mitigate climate change impacts. Modulating root system function provides opportunities to improve crop yield under biotic and abiotic stresses. With this aim, a commercial hybrid tomato variety was grafted on a genotyped population of 123 recombinant inbred lines (RILs) derived from Solanum pimpinellifolium, and compared with self- and non-grafted controls, under contrasting watering treatments (100% vs. 70% of crop evapotranspiration). Drought tolerance was genetically analyzed for vegetative and flowering traits, and root xylem sap phytohormone and nutrient composition. Under water deficit, around 25% of RILs conferred larger total shoot dry weight than controls. Reproductive and vegetative traits under water deficit were highly and positively correlated to the shoot water content. This association was genetically supported by linkage of quantitative trait loci (QTL) controlling these traits within four genomic regions. From a total of 83 significant QTLs, most were irrigation-regime specific. The gene contents of 8 out of 12 genomic regions containing 46 QTLs were found significantly enriched at certain GO terms and some candidate genes from diverse gene families were identified. Thus, grafting commercial varieties onto selected rootstocks derived from S. pimpinellifolium provides a viable strategy to enhance drought tolerance in tomato.

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Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Root-to-Shoot Signaling and Rootstock-Mediated Tolerance to Water Deficit in Tomato

Asíns

Albacete

Martínez‐Andújar

et al. 2020

Genes

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“…Interestingly, transcripts encoding proteins acting on CW polysaccharides and proteases which were well-represented in this set of candidates were down-regulated in Hern. Proteases play roles in the generation of signals involved in plant development, in the maturation of proteins and in protein turnover [ 73 , 74 ]. These processes which are still poorly understood could be important in the phenotypic differentiation of Hern floral stems at 15 °C.…”

Section: Discussionmentioning

confidence: 99%

An Integrative Study Showing the Adaptation to Sub-Optimal Growth Conditions of Natural Populations of Arabidopsis thaliana: A Focus on Cell Wall Changes

Duruflé

Ranocha

Zivy

et al. 2020

Cells

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In the global warming context, plant adaptation occurs, but the underlying molecular mechanisms are poorly described. Studying natural variation of the model plant Arabidopsisthaliana adapted to various environments along an altitudinal gradient should contribute to the identification of new traits related to adaptation to contrasted growth conditions. The study was focused on the cell wall (CW) which plays major roles in the response to environmental changes. Rosettes and floral stems of four newly-described populations collected at different altitudinal levels in the Pyrenees Mountains were studied in laboratory conditions at two growth temperatures (22 vs. 15 °C) and compared to the well-described Col ecotype. Multi-omic analyses combining phenomics, metabolomics, CW proteomics, and transcriptomics were carried out to perform an integrative study to understand the mechanisms of plant adaptation to contrasted growth temperature. Different developmental responses of rosettes and floral stems were observed, especially at the CW level. In addition, specific population responses are shown in relation with their environment and their genetics. Candidate genes or proteins playing roles in the CW dynamics were identified and will deserve functional validation. Using a powerful framework of data integration has led to conclusions that could not have been reached using standard statistical approaches.

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“…Drought is an environmental stress that can negatively impact plant productivity and crop yields ( Gupta et al, 2020 ). Several classes of proteases are involved in the acclimation of plants to drought (reviewed by Vaseva et al, 2011 ; Fanourakis et al, 2020 ). For example, the senescence-associated subtilisin protease (SASP) is a key regulator in abscisic acid (ABA) signaling and drought tolerance ( Acharya et al, 2013 ; Wang et al, 2018b ).…”

Section: Introductionmentioning

confidence: 99%

The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance

Mishra

Caddell

et al. 2021

Front. Plant Sci.

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While drought severely affects plant growth and crop production, the molecular mechanisms of the drought response of plants remain unclear. In this study, we demonstrated for the first time the effect of the pseudo-protease AtFtsHi3 of Arabidopsis thaliana on overall plant growth and in drought tolerance. An AtFTSHi3 knock-down mutant [ftshi3-1(kd)] displayed a pale-green phenotype with lower photosynthetic efficiency and Darwinian fitness compared to wild type (Wt). An observed delay in seed germination of ftshi3-1(kd) was attributed to overaccumulation of abscisic acid (ABA); ftshi3-1(kd) seedlings showed partial sensitivity to exogenous ABA. Being exposed to similar severity of soil drying, ftshi3-1(kd) was drought-tolerant up to 20 days after the last irrigation, while wild type plants wilted after 12 days. Leaves of ftshi3-1(kd) contained reduced stomata size, density, and a smaller stomatic aperture. During drought stress, ftshi3-1(kd) showed lowered stomatal conductance, increased intrinsic water-use efficiency (WUEi), and slower stress acclimation. Expression levels of ABA-responsive genes were higher in leaves of ftshi3-1(kd) than Wt; DREB1A, but not DREB2A, was significantly upregulated during drought. However, although ftshi3-1(kd) displayed a drought-tolerant phenotype in aboveground tissue, the root-associated bacterial community responded to drought.

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The Role of Proteases in Determining Stomatal Development and Tuning Pore Aperture: A Review

Cited by 22 publications

References 84 publications

Genetic Analysis of Root-to-Shoot Signaling and Rootstock-Mediated Tolerance to Water Deficit in Tomato

Genetic Analysis of Root-to-Shoot Signaling and Rootstock-Mediated Tolerance to Water Deficit in Tomato

An Integrative Study Showing the Adaptation to Sub-Optimal Growth Conditions of Natural Populations of Arabidopsis thaliana: A Focus on Cell Wall Changes

The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance

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