The Role of Proteomics in Bacterial Response to Antibiotics

Abstract

For many years, we have tried to use antibiotics to eliminate the persistence of pathogenic bacteria. However, these infectious agents can recover from antibiotic challenges through various mechanisms, including drug resistance and antibiotic tolerance, and continue to pose a global threat to human health. To design more efficient treatments against bacterial infections, detailed knowledge about the bacterial response to the commonly used antibiotics is required. Proteomics is a well-suited and powerful tool to study molecular response to antimicrobial compounds. Bacterial response profiling from system-level investigations could increase our understanding of bacterial adaptation, the mechanisms behind antibiotic resistance and tolerance development. In this review, we aim to provide an overview of bacterial response to the most common antibiotics with a focus on the identification of dynamic proteome responses, and through published studies, to elucidate the formation mechanism of resistant and tolerant bacterial phenotypes.

Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach

“…The disappearance of the signal characteristic of the 50S ribosomal protein L30 ( m/z = 6341.0) was demonstrated. This can result in disturbances in translation and protein synthesis [ 57 ].…”

Evaluation of New Antimicrobial Agents Based on tris(1H-Indol-3-yl)methylium Salts: Activity, Toxicity, Suppression of Experimental Sepsis in Mice

“…A promising direction is the development of tris(indolyl)methylium salts containing maleimide moiety [20]. The latter are known for their ability to suppress various enzymes, including various protein kinases [11][12][13][14], and function as active inhibitors of intracellular signaling [25]. Derivatives of tris(indolyl)methane, for their part, possess the ability to actively form ion channels in the structure of cell membranes and can easily penetrate the microbial cell; thus, they can be considered as carriers, increasing penetration of a target molecule into the microbial cell.…”

“…The situation in the field of therapy of infectious diseases has been significantly complicated due to the wide spread of pathogens resistant to known antibiotic drugs [11][12][13].…”

Antibiotic resistance: Time of synthesis in a post-genomic age

“…Further, it has been shown that antibiotics may induce an uncoordinated response in which transcriptionally and phenotypically important genes are not linked [129] . Nevertheless, this decoupling does not mean that transcriptomic/proteomic data are irrelevant for analysing AR, and there are different examples of their application [130] , [131] , [132] , [133] , [134] . Indeed, these methods have been used to describe new mechanisms of resistance, which have been experimentally validated [135] , and it has been proposed that transcriptome profiling can be a useful tool in the analysis of AR in organisms such as P. aeruginosa [136] .…”