The Role of Pumilio RNA Binding Protein in Plants

Huh, Sung Un

doi:10.3390/biom11121851

Cited by 10 publications

(6 citation statements)

References 63 publications

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“…Members of this gene family are mostly related to plants response to stress, biotic as well as abiotic stress. In biotic stress expression of Pum genes is related to plants immunity (Huh, 2021) and synthesis of antibacterial peptides (Gerber et al, 2006). Expression of Pum genes is also changed under salt, cold or drought stress and upregulated expression of these genes can enhance heat stress tolerance (Nyiko et al, 2019).…”

Section: Identification Of Candidate Genes Involved In Primed Memory ...mentioning

confidence: 99%

RNA Sequencing indicates gene expression changes in Silene sendtneri seeds after seed priming with silicic acid

Karalija

Demira

Samardžić

et al. 2022

GenApp

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To improve our understanding of the molecular mechanisms underlaying seed priming, RNA transcriptome analysis was performed using primed and non-primed seeds of Silene sendtneri. Seed priming was performed by submergence in 1% silicic acid for 24h at 4°C, followed by rinsing with sterilised water and desiccation to original moisture content. Silene sendtneri is a species with no sequenced genome and annotation of de novo assembly of transcriptome was done against several species. Gene ontology (GO) analysis indicated that genes related to heavy metal transporters and heat shock proteins are differentially expressed after priming with silicic acid. Within these gene categories, genes such as heavy metal-associated isoprenylated plant protein 26-like (log2fold -8.79) were downregulated, while others such as heavy metal ATPase 5 (log2fold 6.46), heat shock factor protein HSF30-like isoform X1 (log2fold 5.98) were upregulated.

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Section: Identification Of Candidate Genes Involved In Primed Memory ...mentioning

confidence: 99%

RNA Sequencing indicates gene expression changes in Silene sendtneri seeds after seed priming with silicic acid

Karalija

Demira

Samardžić

et al. 2022

GenApp

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“…Protein interactions in general, and those of PUF (Pumilio and FBF) family members in particular, can be organism‐, transcript‐, tissue‐, and even condition‐specific (Marrero et al , 2011 ). Pumilio was discovered over 20 years ago to serve crucial roles in germline stem cell maintenance and embryogenesis in Drosophila and C. elegans (Wickens et al , 2002 ), and studies of its targets reveal it to be involved in development, host defense, and response to stressors in yeast and plants (Wickens et al , 2002 ; Miles et al , 2012 ; Goldstrohm et al , 2018 ; Uyhazi et al , 2020 ; Huh, 2021 ). Almost nothing is known about Pum1 function in the mammalian brain, however, beyond embryonic neurogenesis (Zhang et al , 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Dosage sensitivity to Pumilio1 variants in the mouse brain reflects distinct molecular mechanisms

et al. 2023

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Different mutations in the RNA‐binding protein Pumilio1 (PUM1) cause divergent phenotypes whose severity tracks with dosage: a mutation that reduces PUM1 levels by 25% causes late‐onset ataxia, whereas haploinsufficiency causes developmental delay and seizures. Yet PUM1 targets are derepressed to equal degrees in both cases, and the more severe mutation does not hinder PUM1's RNA‐binding ability. We therefore considered the possibility that the severe mutation might disrupt PUM1 interactions, and identified PUM1 interactors in the murine brain. We find that mild PUM1 loss derepresses PUM1‐specific targets, but the severe mutation disrupts interactions with several RNA‐binding proteins and the regulation of their targets. In patient‐derived cell lines, restoring PUM1 levels restores these interactors and their targets to normal levels. Our results demonstrate that dosage sensitivity does not always signify a linear relationship with protein abundance but can involve distinct mechanisms. We propose that to understand the functions of RNA‐binding proteins in a physiological context will require studying their interactions as well as their targets.

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“…Although Pum proteins show high conservation in the Pum-HD sequence, there is a high diversity of Pum gene members in plants. For example, there are 2, 2, 2, 6, 10, and 11 Pum genes in the genome of Drosophila melanogaster , human, mouse, Saccharomyces cerevisiae , Trypanosoma cruzi , and Caenorhabditis elegans , respectively [ 8 , 11 , 23 , 24 ]. On the contrary, there are 31, 26, 28, 26, 22, and 20 Pum genes in the genome of Arabidopsis lyrata , Arabidopsis thaliana , Malus domestica , Glycine max , Oryza sativa ssp.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Identification of the Pum Gene Family and Its Involvement in Kernel Development in Maize

Feng,

Zhang,

Cao

et al. 2023

IJMS

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The Pumilio (Pum) RNA-binding protein family regulates post-transcription and plays crucial roles in stress response and growth. However, little is known about Pum in plants. In this study, a total of 19 ZmPum genes were identified and classified into two groups in maize. Although each ZmPum contains the conserved Pum domain, the ZmPum members show diversity in the gene and protein architectures, physicochemical properties, chromosomal location, collinearity, cis-elements, and expression patterns. The typical ZmPum proteins have eight α-helices repeats, except for ZmPum2, 3, 5, 7, and 14, which have fewer α-helices. Moreover, we examined the expression profiles of ZmPum genes and found their involvement in kernel development. Except for ZmPum2, ZmPum genes are expressed in maize embryos, endosperms, or whole seeds. Notably, ZmPum4, 7, and 13 exhibited dramatically high expression levels during seed development. The study not only contributes valuable information for further validating the functions of ZmPum genes but also provides insights for improvement and enhancing maize yield.

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The Role of Pumilio RNA Binding Protein in Plants

Cited by 10 publications

References 63 publications

RNA Sequencing indicates gene expression changes in Silene sendtneri seeds after seed priming with silicic acid

RNA Sequencing indicates gene expression changes in Silene sendtneri seeds after seed priming with silicic acid

Dosage sensitivity to Pumilio1 variants in the mouse brain reflects distinct molecular mechanisms

Comprehensive Identification of the Pum Gene Family and Its Involvement in Kernel Development in Maize

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