The Role of Reactive Oxygen Species and Calcium Ions in the Implementation of the Stress-Protective Effect of Brassinosteroids on Plant Cells

Kolupaev, Yu. Е.; Vayner, A. A.; Yastreb, Т. О.; Oboznyi, A. I.; Хрипач, В. А.

doi:10.7868/s0555109914060075

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Decrease of mitochondrial electron transport is due, in part, to oxidative injury and change in intracellular Ca 2+ concentration (Kolupaev et al, 2014). In the early phase of apoptosis, mitochondrial Ca 2+ release leads to an increase in intracellular Ca 2+ levels (Akopova et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Mechanism of juglone-induced apoptosis of MCF-7 cells by the mitochondrial pathway

Yu-bin

Xin

et al. 2016

Genet. Mol. Res.

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ABSTRACT. This study investigated the nature and mechanism of juglone-induced apoptosis in the human breast cancer cell line MCF-7. The inhibitory effect of juglone on MCF-7 cell growth was evaluated by the dimethylthiazol tetrazolium assay. Morphological apoptotic changes were characterized using an inverted microscope, Hoechst 33258 fluorescence staining, and Giemsa staining. The rate of cell apoptosis, intracellular levels of reactive oxygen species (ROS), and mitochondrial membrane potential were detected using flow cytometry. Intracellular Ca 2+ concentrations were detected using laser scanning confocal fluorescence microscopy. Expression of the proteins Bcl-2, Bax, and cytochrome C was assessed by western blotting. Caspase-3 activity was quantified using a caspase-3 activity kit. Juglone inhibited the growth of MCF-7 cell line with an IC50 of 11.99 µM. The rates of MCF-7 cell apoptosis at 24 h after exposure to 5, 10, and 20 µM juglone were 9.29, 20.67, and 28.39%, respectively; compared to unexposed cells, juglone-exposed cells exhibited significant elevation in intracellular ROS level, decrease in mitochondrial membrane potential, and increase in intracellular Ca 2+ concentration. Juglone upregulated the expression of Bax, and downregulated the expression of Bcl-2, promoting the release of cytochrome C, thereby upregulating the activity of caspase-3. The results suggest that the mechanism of juglone-induced apoptosis in MCF-7 cells is characterized by elevated ROS levels, reduced Bcl-2 expression, increased Bax expression, decreased mitochondrial membrane potential, increased intracellular Ca 2+ concentration, outer mitochondrial-membrane rupture, cytochrome C release, and caspase-3 activation.

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Section: Discussionmentioning

confidence: 99%

Mechanism of juglone-induced apoptosis of MCF-7 cells by the mitochondrial pathway

Yu-bin

Xin

et al. 2016

Genet. Mol. Res.

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Mechanisms of cytosolic calcium elevation in plants: the role of ion channels, calcium extrusion systems and NADPH oxidase-mediated 'ROS-Ca2+ Hub'

Demidchik

Shabala

2018

Functional Plant Biol.

117

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Elevation in the cytosolic free calcium is crucial for plant growth, development and adaptation. Calcium influx into plant cells is mediated by Ca2+ depolarisation-activated, hyperpolarisation-activated and voltage-independent Ca2+-permeable channels (DACCs, HACCs and VICCs respectively). These channels are encoded by the following gene families: (1) cyclic nucleotide-gated channels (CNGCs), (2) ionotropic glutamate receptors (GLRs), (3) annexins, (4) ‘mechanosensitive channels of small (MscS) conductance’-like channels (MSLs), (5) ‘mid1-complementing activity’ channels (MCAs), Piezo channels, and hyperosmolality-induced [Ca2+]cyt. channel 1 (OSCA1). Also, a ‘tandem-pore channel1’ (TPC1) catalyses Ca2+ efflux from the vacuole in response to the plasma membrane-mediated Ca2+ elevation. Recent experimental data demonstrated that Arabidopsis thaliana (L.) Heynh. CNGCs 2, 5–10, 14, 16 and 18, GLRs 1.2, 3.3, 3.4, 3.6 and 3.7, TPC1, ANNEXIN1, MSL9 and MSL10,MCA1 and MCA2, OSCA1, and some their homologues counterparts in other species, are responsible for Ca2+ currents and/or cytosolic Ca2+ elevation. Extrusion of Ca2+ from the cytosol is mediated by Ca2+-ATPases and Ca2+/H+ exchangers which were recently examined at the level of high resolution crystal structure. Calcium-activated NADPH oxidases and reactive oxygen species (ROS)-activated Ca2+ conductances form a self-amplifying ‘ROS-Ca2+hub’, enhancing and transducing Ca2+ and redox signals. The ROS-Ca2+ hub contributes to physiological reactions controlled by ROS and Ca2+, demonstrating synergism and unity of Ca2+ and ROS signalling mechanisms.

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Calcium transport across plant membranes: mechanisms and functions

et al. 2018

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Contents Summary 49 I. Introduction 49 II. Physiological and structural characteristics of plant Ca -permeable ion channels 50 III. Ca extrusion systems 61 IV. Concluding remarks 64 Acknowledgements 64 References 64 SUMMARY: Calcium is an essential structural, metabolic and signalling element. The physiological functions of Ca are enabled by its orchestrated transport across cell membranes, mediated by Ca -permeable ion channels, Ca -ATPases and Ca /H exchangers. Bioinformatics analysis has not determined any Ca -selective filters in plant ion channels, but electrophysiological tests do reveal Ca conductances in plant membranes. The biophysical characteristics of plant Ca conductances have been studied in detail and were recently complemented by molecular genetic approaches. Plant Ca conductances are mediated by several families of ion channels, including cyclic nucleotide-gated channels (CNGCs), ionotropic glutamate receptors, two-pore channel 1 (TPC1), annexins and several types of mechanosensitive channels. Key Ca -mediated reactions (e.g. sensing of temperature, gravity, touch and hormones, and cell elongation and guard cell closure) have now been associated with the activities of specific subunits from these families. Structural studies have demonstrated a unique selectivity filter in TPC1, which is passable for hydrated divalent cations. The hypothesis of a ROS-Ca hub is discussed, linking Ca transport to ROS generation. CNGC inactivation by cytosolic Ca , leading to the termination of Ca signals, is now mechanistically explained. The structure-function relationships of Ca -ATPases and Ca /H exchangers, and their regulation and physiological roles are analysed.

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The Role of Reactive Oxygen Species and Calcium Ions in the Implementation of the Stress-Protective Effect of Brassinosteroids on Plant Cells

Cited by 3 publications

References 24 publications

Mechanism of juglone-induced apoptosis of MCF-7 cells by the mitochondrial pathway

Mechanism of juglone-induced apoptosis of MCF-7 cells by the mitochondrial pathway

Mechanisms of cytosolic calcium elevation in plants: the role of ion channels, calcium extrusion systems and NADPH oxidase-mediated 'ROS-Ca2+ Hub'

Calcium transport across plant membranes: mechanisms and functions

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