The Role of Red Blood Cell Deformability and Na,K-ATPase Function in Selected Risk Factors of Cardiovascular Diseases in Humans: Focus on Hypertension, Diabetes Mellitus and Hypercholesterolemia

Radošinská, Jana; Vrbjar, N

doi:10.33549/physiolres.933402

Cited by 73 publications

(61 citation statements)

References 78 publications

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“…Besides, plasma NO increases RBC velocity through microvascular beds in vivo by regulating vascular tone and modifying RBC deformability . Importantly, RBC deformability may affect microcirculatory blood flow and oxygen delivery to tissues, and impaired RBC deformability independently predicts adverse outcomes in cardiovascular diseases and sepsis . Our results indicated that high‐dose NTG may benefit tissue perfusion of cardiac surgical patients through improving RBC deformability and function of cytoskeletal structural proteins during CPB.…”

Section: Discussionmentioning

confidence: 74%

High‐dose nitroglycerin administered during rewarming preserves erythrocyte deformability in cardiac surgery with cardiopulmonary bypass

Tai

Chu

et al. 2020

Microcirculation

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Objective:We aimed to determine whether high-dose nitroglycerin, a nitric oxide donor, preserves erythrocyte deformability during cardiopulmonary bypass and examines the signaling pathway of nitric oxide in erythrocytes. Methods:In a randomized and controlled fashion, forty-two patients undergoing cardiac surgery with hypothermic cardiopulmonary bypass were allocated to high-dose (N = 21) and low-dose groups (N = 21). During rewarming period, patients were given intravenous nitroglycerin with an infusion rate 5 and 1 µg·kg −1 ·min −1 in high-dose and low-dose groups, respectively. Tyrosine phosphorylation level of non-muscle myosin IIA in erythrocyte membrane was used as an index of erythrocyte deformability and analyzed using immunoblotting.Results: Tyrosine phosphorylation of non-muscle myosin IIA was significantly enhanced after bypass in high-dose group (3.729 ± 1.700 folds, P = .011) but not low-dose group (1.545 ± 0.595 folds, P = .076). Phosphorylation of aquaporin 1, vasodilator-stimulated phosphoprotein, and focal adhesion kinase in erythrocyte membrane was also upregulated in high-dose group after bypass. Besides, plasma nitric oxide level was highly correlated with fold change of non-muscle myosin IIA phosphorylation (Pearson's correlation coefficient .871). Conclusions: High-dose nitroglycerin administered during cardiopulmonary bypass improves erythrocyte deformability through activating phosphorylation of aquaporin 1, vasodilator-stimulated phosphoprotein, and focal adhesion kinase in erythrocytes. K E Y W O R D S aquaporin 1, focal adhesion kinase, nitric oxide, non-muscle myosin IIA, vasodilatorstimulated phosphoprotein S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section.

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Section: Discussionmentioning

confidence: 74%

High‐dose nitroglycerin administered during rewarming preserves erythrocyte deformability in cardiac surgery with cardiopulmonary bypass

Tai

Chu

et al. 2020

Microcirculation

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“…Показано, что прогрессирование ряда заболеваний сопровождается морфофункциональными изменениями форменных элементов крови, в том числе, эритроцитов [26]. Более поздние исследования позволили предположить, что нарушение функционального состояния эритроцитов (изменение их деформируемости) можно рассматривать как один из патогенетических механизмов развития МС [27].…”

Section: функциональные свойства эритроцитовunclassified

Abnormal Microcirculation and Red Blood Cell Function as a Cardiovascular Risk Factor in Metabolic Syndrome

Подзолков

Королева

Pisarev

et al. 2018

Racionalʹnaâ farmakoterapiâ v kardiologii

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The metabolic syndrome is a highly prevalent condition associated with increased cardiovascular risk in the population. Microvasculature is the terminal part of the cardiovascular system which primarily reacts to the increased secretion of the pro inflammatory adipokines typical for the metabolic syndrome. Microcirculation and blood cell abnormalities are the leading mechanisms of cardiovascular events development in this condition. Prevalence of microcirculation abnormalities and red blood cell dysfunction in metabolic syndrome and their role in the increased blood viscosity and cardiovascular events development are covered by the paper. The microcirculation abnormalities with a special focus on red blood cell dysfunction (impaired aggregation, stiffness) seen in metabolic syndrome and associated conditions are discussed in detail. The impact of abnormal red blood cell deformability (secondary to cholesterol accumulation in the cellular membranes) on the hemorheological abnormalities is revealed. Abnormal red blood cell surface charge due to proinflammatory changes associated with insulin resistance in diabetes mellitus is highlighted. These abnormalities lead to increased red blood cell aggregation and plasma viscosity that are the essential components of cardiovascular events pathogenesis. Their timely diagnosis is crucial for effective cardiovascular prevention.

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“…The mechanical analysis of a cell population, such as a blood sample, enables meaningful biological and medical interpretation for diagnosis and monitoring of diseases. Malaria, [10][11][12] diabetes, 13,14 hypercholesterolemia 14,15 and many others are examples of widespread blood diseases that alter the mechanics of human erythrocytes also called red blood cells (RBCs). 16,17 Most methods for mechanical analysis provide number averaged values such as the blood's viscosity.…”

Section: Introductionmentioning

confidence: 99%

Acoustic erythrocytometer for mechanically probing cell viscoelasticity

Link

Franke

2020

Lab Chip

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We demonstrate an acoustic device to mechanically probe a population of red blood cells at the single cell level. The device operates by exciting a surface acoustic wave in a microfluidic channel creating a stationary acoustic wave field of nodes and antinodes. Erythrocytes are attracted to the nodes and are deformed. Using a stepwise increasing and periodically oscillating acoustic field we study the static and dynamic deformation of individual red blood cells one by one. We quantify the deformation by the Taylor deformation index D and relaxation times τ 1 and τ 2 that reveal both the viscous and elastic properties of the cells. The precision of the measurement allows us to distinguish between individual cells in the suspension and provides a quantitative viscoelastic fingerprint of the blood sample at single cell resolution. The method overcomes limitations of other techniques that provide averaged values and has the potential for high-throughput. † Electronic supplementary information (ESI) available: Movie showing the surface acoustic wave actuated deformation of a red blood cell for stepwise increasing power amplitudes. See

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The Role of Red Blood Cell Deformability and Na,K-ATPase Function in Selected Risk Factors of Cardiovascular Diseases in Humans: Focus on Hypertension, Diabetes Mellitus and Hypercholesterolemia

Cited by 73 publications

References 78 publications

High‐dose nitroglycerin administered during rewarming preserves erythrocyte deformability in cardiac surgery with cardiopulmonary bypass

High‐dose nitroglycerin administered during rewarming preserves erythrocyte deformability in cardiac surgery with cardiopulmonary bypass

Abnormal Microcirculation and Red Blood Cell Function as a Cardiovascular Risk Factor in Metabolic Syndrome

Acoustic erythrocytometer for mechanically probing cell viscoelasticity

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