This study explored how heterosexual and lesbian women differ in their implicit sexual responses. Previous research indicates that heterosexual women have physiological and implicit responses to both genders, whereas lesbian women show stronger responses to their preferred gender. This study used two implicit measures: the Implicit Relational Assessment Procedure (IRAP) and the Function Acquisition Speed Test (FAST), both of which were novel in this context. We recruited 33 heterosexual and 25 lesbian women. Both IRAP and FAST were successful in differentiating the two sexual orientations as a group. The results confirmed that heterosexual women exhibit positive responses to both genders, while lesbian women show stronger, category-specific responses to their preferred gender. These findings align with previous research and provide further insight into the nuanced differences in sexual orientation responses among women.