The Role of Rhizobacterial Volatile Organic Compounds in a Second Green Revolution—The Story so Far

Heenan-Daly, Darren; Velivelli, Siva L. S.; Prestwich, Barbara Doyle

doi:10.1007/978-3-030-30926-8_8

Cited by 3 publications

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“…Volatile compounds, particularly volatile organic compounds, often serve as chemical communication signals in both intra- and interspecific communication between kingdoms ( Schulz-Bohm et al, 2017 ). Of the numerous activities that rhizobacteria employ to aid plant growth, BVCs have emerged as a key area of investigation over the last two decades ( Ryu et al, 2003 ; Farag et al, 2006 , 2017 ) and their future application to agricultural production systems are an attractive option, especially with regard to sustainable agriculture ( Heenan-Daly et al, 2019 ; Garbeva and Weisskopf, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Volatile Compounds From Bacillus, Serratia, and Pseudomonas Promote Growth and Alter the Transcriptional Landscape of Solanum tuberosum in a Passively Ventilated Growth System

et al. 2021

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The interaction of an array of volatile organic compounds (VOCs) termed bacterial volatile compounds (BVCs) with plants is now a major area of study under the umbrella of plant-microbe interactions. Many growth systems have been developed to determine the nature of these interactions in vitro. However, each of these systems have their benefits and drawbacks with respect to one another and can greatly influence the end-point interpretation of the BVC effect on plant physiology. To address the need for novel growth systems in BVC-plant interactions, our study investigated the use of a passively ventilated growth system, made possible via Microbox® growth chambers, to determine the effect of BVCs emitted by six bacterial isolates from the genera Bacillus, Serratia, and Pseudomonas. Solid-phase microextraction GC/MS was utilized to determine the BVC profile of each bacterial isolate when cultured in three different growth media each with varying carbon content. 66 BVCs were identified in total, with alcohols and alkanes being the most abundant. When cultured in tryptic soy broth, all six isolates were capable of producing 2,5-dimethylpyrazine, however BVC emission associated with this media were deemed to have negative effects on plant growth. The two remaining media types, namely Methyl Red-Voges Proskeur (MR-VP) and Murashige and Skoog (M + S), were selected for bacterial growth in co-cultivation experiments with Solanum tuberosum L. cv. ‘Golden Wonder.’ The BVC emissions of Bacillus and Serratia isolates cultured on MR-VP induced alterations in the transcriptional landscape of potato across all treatments with 956 significantly differentially expressed genes. This study has yielded interesting results which indicate that BVCs may not always broadly upregulate expression of defense genes and this may be due to choice of plant-bacteria co-cultivation apparatus, bacterial growth media and/or strain, or likely, a complex interaction between these factors. The multifactorial complexities of observed effects of BVCs on target organisms, while intensely studied in recent years, need to be further elucidated before the translation of lab to open-field applications can be fully realized.

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