The role of Rho1 gene in the cell wall integrity and polysaccharides biosynthesis of the edible mushroom Grifola frondosa

Zan, Xinyi; Zhu, Hong-An; Jiang, Lihua; Liang, Yingying; Sun, Wenjing; Tao, Ting-Lei; Cui, Fengjie

doi:10.1016/j.ijbiomac.2020.09.239

Cited by 15 publications

(6 citation statements)

References 34 publications

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“…In particular, restoration of Rho1 was the highest and increased by 14.24-fold (Figure B). Previous studies have found that Rho1 not only integrates signals from the cell surface and the cell division cycle but also regulates a variety of outputs involved in cell wall biogenesis, actin organization, and polarized secretion. , Interruptions in Rho1 expression cause the reduction of cell wall β-1,3-glucan and increase the sensitivity of Aspergillus fumigatus to multiple stresses . Therefore, serine supplementation could positively regulate the CWI pathway to remodel the cell wall of Z.…”

Section: Resultsmentioning

confidence: 99%

Serine Improves Lactic Acid Stress Tolerance and Ethanol Production in Zygosaccharomyces bailii in Baijiu Fermentation

Wei,

Nie,

et al. 2023

J. Agric. Food Chem.

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Section: Resultsmentioning

confidence: 99%

Serine Improves Lactic Acid Stress Tolerance and Ethanol Production in Zygosaccharomyces bailii in Baijiu Fermentation

Wei,

Nie,

et al. 2023

J. Agric. Food Chem.

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“…Genes from the amino sugar and nucleotide sugar metabolism KEGG pathway (00520) had been proved to be involved in the synthesis of nucleotide-activated sugars [ 64 ]. Research papers have shown that genes which are involved in the MAPK signaling pathway—yeast KEGG pathway participate in the regulation of exopolysaccharide synthesis [ 65 ]. In this study, we also found genes mapped to these two KEGG pathways in the G. lucidum genome.…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing and Transcriptome Analysis of Ganoderma lucidum Strain Yw-1-5 Provides New Insights into the Enhanced Effect of Tween80 on Exopolysaccharide Production

Cai

et al. 2022

JoF

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Ganoderma lucidum is an important medicinal mushroom widely cultured in Asian countries. Exopolysaccharides are bioactive compounds of G. lucidum with health benefits. Limited exopolysaccharide content hinders its extraction from G. lucidum. The addition of Tween80 had an enhanced effect on G. lucidum exopolysaccharide production in submerged fermentation. However, the mechanism of this effect remains unclear. In this study, we report on a high-quality assembly of G. lucidum strain yw-1-5 to lay the foundation for further transcriptome analysis. The genome sequence was 58.16 Mb and consisted of 58 scaffolds with an N50 of 4.78 Mb. A total of 13,957 protein-coding genes were annotated and Hi-C data mapped to 12 pseudo-chromosomes. Genes encoding glycosyltransferases and glycoside hydrolases were also obtained. Furthermore, RNA-seq was performed in a Tween80-treated group and control group for revealing the enhanced effect of Tween80 on exopolysaccharide production. In total, 655 genes were identified as differentially expressed, including 341 up-regulated and 314 down-regulated. Further analysis of differentially expressed genes showed that groups of MAPK, amino sugar and nucleotide sugar metabolism, autophagy, ubiquitin-mediated proteolysis, peroxisome, starch and sucrose metabolism, TCA cycle, glycolysis/gluconeogenesis KEGG pathway, glycosyltransferases and glycoside hydrolases played important roles in the enhanced effect of Tween80 on exopolysaccharide production. This work provides a valuable resource for facilitating our understanding of the synthesis of polysaccharides and accelerating the breeding of new strains with a high content of exopolysaccharides.

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“…The basidiomycete Ustilago maydis causes corn smut disease; an Rho1 homolog of U. maydis is required for vegetative growth and is associated with cell polarity and cytokinesis, and Rho1 loss results in abnormalities in budding and chitin deposition [ 67 ]. In another basidiomycete, the edible mushroom Grifola frondosa , loss of Rho1 results in reduced mycelial growth, decreased amount of cell wall polysaccharides, and increased sensitivity to cell wall stress [ 68 ].…”

Section: Signal Transduction Downstream Of Cell Surface Sensorsmentioning

confidence: 99%

Cell Wall Integrity and Its Industrial Applications in Filamentous Fungi

Yoshimi,

Miyazawa,

Kawauchi

et al. 2022

JoF

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Signal transduction pathways regulating cell wall integrity (CWI) in filamentous fungi have been studied taking into account findings in budding yeast, and much knowledge has been accumulated in recent years. Given that the cell wall is essential for viability in fungi, its architecture has been analyzed in relation to virulence, especially in filamentous fungal pathogens of plants and humans. Although research on CWI signaling in individual fungal species has progressed, an integrated understanding of CWI signaling in diverse fungi has not yet been achieved. For example, the variety of sensor proteins and their functional differences among different fungal species have been described, but the understanding of their general and species-specific biological functions is limited. Our long-term research interest is CWI signaling in filamentous fungi. Here, we outline CWI signaling in these fungi, from sensor proteins required for the recognition of environmental changes to the regulation of cell wall polysaccharide synthesis genes. We discuss the similarities and differences between the functions of CWI signaling factors in filamentous fungi and in budding yeast. We also describe the latest findings on industrial applications, including those derived from studies on CWI signaling: the development of antifungal agents and the development of highly productive strains of filamentous fungi with modified cell surface characteristics by controlling cell wall biogenesis.

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The role of Rho1 gene in the cell wall integrity and polysaccharides biosynthesis of the edible mushroom Grifola frondosa

Cited by 15 publications

References 34 publications

Serine Improves Lactic Acid Stress Tolerance and Ethanol Production in Zygosaccharomyces bailii in Baijiu Fermentation

Serine Improves Lactic Acid Stress Tolerance and Ethanol Production in Zygosaccharomyces bailii in Baijiu Fermentation

Whole-Genome Sequencing and Transcriptome Analysis of Ganoderma lucidum Strain Yw-1-5 Provides New Insights into the Enhanced Effect of Tween80 on Exopolysaccharide Production

Cell Wall Integrity and Its Industrial Applications in Filamentous Fungi

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