The role of Roquin overexpression in the modulation of signaling during in vitro and ex vivo T-cell activation

Kim, Hei Jung; Ji, Young Rae; Kim, Myoung Ok; Yu, Dong Hoon; Shin, Mi Jung; Yuh, Hyung Soo; Bae, Ki Beom; Park, Seo Jin; Yi, Jun Koo; Kim, Na Ri; Park, Si Jun; Yoon, D. H.; Lee, Won Ha; Lee, Sanggyu; Ryoo, Zae Young

doi:10.1016/j.bbrc.2011.11.101

Cited by 11 publications

(18 citation statements)

References 21 publications

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“…Genomic DNA analysis of the dead fetuses revealed that they were transgenic mice (Figure 2A). Furthermore, analysis of the litter size and birth rate revealed that littermates of PlGF transgenic mice were smaller than WT mice and control transgenic mice that expressed another gene 19 by the human CD-2 promoter ( Figure 2B and 2C). Interestingly, the birth rate of transgenic mice did not follow Mendelian inheritance.…”

Section: Defects Of Litter Size and Weight Gain In Plgf Overexpressinmentioning

confidence: 99%

Gestational Loss and Growth Restriction by Angiogenic Defects in Placental Growth Factor Transgenic Mice

Kang

Park

Kim

et al. 2014

ATVB

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Objective-Angiogenesis is an important biological process during development, reproduction, and in immune responses.Placental growth factor (PlGF) is a member of vascular endothelial growth factor that is critical for angiogenesis and vasculogenesis. We generated transgenic mice overexpressing PlGF in specifically T cells using the human CD2-promoter to investigate the effects of PlGF overexpression. Approach and Results-Transgenic mice were difficult to obtain owing to high lethality; for this reason, we investigated why gestational loss occurred in these transgenic mice. Here, we report that placenta detachment and inhibition of angiogenesis occurred in PlGF transgenic mice during the gestational period. Moreover, even when transgenic mice were born, their growth was restricted. Conclusions-Conclusively, PlGF overexpression prevents angiogenesis by inhibiting Braf, extracellular signal-regulated kinase activation, and downregulation of HIF-1α in the mouse placenta. Furthermore, it affected regulatory T cells, which are important for maintenance of pregnancy. (Arterioscler Thromb Vasc Biol. 2014;34:2276-2282.)

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Section: Defects Of Litter Size and Weight Gain In Plgf Overexpressinmentioning

confidence: 99%

Gestational Loss and Growth Restriction by Angiogenic Defects in Placental Growth Factor Transgenic Mice

Kang

Park

Kim

et al. 2014

ATVB

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“…However, in our previous study, Roquin overexpression induced up-regulation of CD28 expression as well as downregulation of ICOS during T cell activation by anti-CD3/CD28. Secretion of IL-2 and proinflammatory cytokines was increased by enforced expression of Roquin in T cell activation (21). Thus, decreased ICOS expression may repress Th cell function and prevent pathogenic immune responses.…”

mentioning

confidence: 90%

“…Mice-Roquin transgenic mice were generated as described previously (21). Six generations of Roquin transgenic mice were backcrossed onto the DBA/1 background.…”

Section: Methodsmentioning

confidence: 99%

“…We isolated serum from mice at 20 and 45 days after immunization and then assessed the levels of various cytokines by ELISAs. In a previous study, CD28-induced IL-2 secretion was increased by Roquin overexpression in vitro (21). To confirm whether IL-2 secretion is increased in autoimmunity as it is in vivo, we performed an ELISA on serum collected from CIA mice at 20 and 45 days after immunization.…”

Section: Induction Of Proinflammatory Cytokine Production and Signalimentioning

confidence: 99%

“…ϩ T Cells in Naive Mice-To generate transgenic mouse lines that expressed high levels of Roquin specifically in T cells, mouse Roquin cDNA was inserted into a vector containing a human CD2 transgene cassette (21). To confirm the expression of the transgene, Western blotting was used to monitor production of the Roquin-HA fusion protein in the spleens of Roquin-overexpressing transgenic (Tg) mice (Fig.…”

Section: Generation Of Roquin Transgenic Mice and Modulation Of Co-stmentioning

confidence: 99%

See 2 more Smart Citations

Enforced Expression of Roquin Protein in T Cells Exacerbates the Incidence and Severity of Experimental Arthritis

Kim

et al. 2012

Journal of Biological Chemistry

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The role of Roquin overexpression in the modulation of signaling during in vitro and ex vivo T-cell activation

Cited by 11 publications

References 21 publications

Gestational Loss and Growth Restriction by Angiogenic Defects in Placental Growth Factor Transgenic Mice

Gestational Loss and Growth Restriction by Angiogenic Defects in Placental Growth Factor Transgenic Mice

Enforced Expression of Roquin Protein in T Cells Exacerbates the Incidence and Severity of Experimental Arthritis

Over-expression of Roquin aggravates T cell mediated hepatitis in transgenic mice using T cell specific promoter

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