The role of rosemary extract in degeneration of hippocampal neurons induced by kainic acid in the rat: A behavioral and histochemical approach

Abstract

Systemic Kainic Acid (KA) administration has been used to induce experimental temporal lobe epilepsy in rats. The aim of this study was to evaluate the neuroprotective effect of rosemary extract (RE, 40% Carnosic acid) against KA-induced neurotoxicity in hippocampus and impaired learning and memory. Animals received a single dose of KA (9.5 mg/kg) intraperitoneally (i.p.) (KA group) and were observed for 2 h and were scored from 0 (for normal, no convulsion) to 5 (for continuous generalized limbic seizures). RE (100 mg/kg, orally) was administered daily for 23 days, starting a week before KA injection (KA+RE group). Neuronal degeneration in hippocampus was demonstrated by using Fluoro-Jade B immunofluorescence. The number of pyramidal cells in hippocampus was evaluated by Nissl staining. Also, the Morris Water Maze and Shuttle box have been used to assess spatial memory and passive avoidance learning, respectively. Our results revealed that, after treatment with RE, neuronal loss in CA1 decreased significantly in the animals in KA+RE group. The Morris water navigation task results revealed that spatial memory impairment decreased in the animals in KA+RE group. Furthermore, results in Shuttle box test showed that passive avoidance learning impairment significantly, upgraded in the animals in KA+RE group. These results suggest that RE may improve the spatial and working memory deficits and also neuronal degeneration induced by toxicity of KA in the rat hippocampus, due to its antioxidant activities.

Mediterranean Natural Extracts Improved Cognitive Behavior in Zebrafish and Healthy Rats and Ameliorated LPS-induced Cognitive Impairment in a Sex Dependent Manner

“…In a similar study, GGRE improved memory functions in a mice model of LPS-induced neuroin ammation (41). Moreover, RE has been shown to rescue learning and spatial memory de cits in a rat model of epilepsy induced by treatment with kainic acid (42).…”

Danggui Sini decoction protects against oxaliplatin-induced peripheral neuropathy in rats

“…We employed an effective Chinese Medicine, DSD, for treating oxaliplatin-induced side-effects in DRG cells. Clinically, the oxaliplatin-induced side-effects mainly include electrophysiological characteristic changes, inflammatory response, Nissl body depletion, and damaged ultra-microstructures, all of which are associated with peripheral neuropathy or neurotoxicity (Coriat et al, 2014;Kono et al, 2015;Naderali et al, 2018). However, all of these side-effects always occur in patients suffering from cancers and undergoing treatment of OXAL.…”

Effect of Aluminium and Aqueous extract of Rosmarinus officinalis on rat Brain: Impact on Neurobehavioral and Histological study

“…This is manifested by adecrease in the number of cellular units relative to controls, fibrosis and vacuolation of neuronal cells in the cerebral cortex, as well as necrosis of pyramidal cells in the CA1 region of hippocampus (Al-B). The hippocampus and the cerebral cortex are the key structures of memory formation 55 , which could imply that morphologic abnormalities could partially explain the deficits of memory performances caused by Al.…”