The role of seminal plasma in the liquid storage of spermatozoa

Höfner, Lisa; Luther, Anne-Marie; Waberski, Dagmar

doi:10.1016/j.anireprosci.2020.106290

Cited by 27 publications

(28 citation statements)

References 61 publications

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“…As shown previously for extended semen portions [3,4], the sensitivity of stored boar sperm to SP is time-and concentration-dependent affecting sperm motility, mitochondrial activity, and, to a lesser extent, the membrane integrity. Here, we tested effects of SP in standard semen doses as used in many European AI stations (18 × 10 6 sperm/mL), taking into account that lower sperm concentrations render sperm more susceptible to the long-term effect of SP [3].…”

Section: Discussionsupporting

confidence: 69%

“…Consequently, there is an unphysiological prolonged exposure of sperm to SP during in vitro storage for several days. Previous studies demonstrated a dose-and time-dependent depressing effect of boar SP on motility, which becomes increasingly apparent after 72 h of storage [3,4]. Thus, removal of SP prior to long-term liquid storage can be beneficial for maintaining sperm motility [3] and fertilising ability [5], but seems not to be applicable in AI practice.…”

Section: Introductionmentioning

confidence: 98%

“…Furthermore, this is influenced by environmental factors [10]. Moreover, a low sperm concentration in the semen dose makes sperm more susceptible to adverse effects of SP during in vitro storage [3] and a protective extender can counteract the SP-induced loss of sperm function [4].…”

Section: Introductionmentioning

confidence: 99%

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Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Höfner

Luther

Palladini

et al. 2020

IJMS

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Long-term exposure of liquid preserved boar spermatozoa to seminal plasma (SP) can cause dramatic sperm injury. This study examined whether boar specificity exists in the sensitivity of spermatozoa to SP and whether correspondent biomarkers can be identified. Consecutive ejaculates (n = 4–5) collected from 19 boars were centrifuged, diluted with a pH-stablising extender with 10% (v/v) autologous SP and evaluated by computer-assisted semen analysis and flow cytometry. Up until 144 h storage, four boars showed consistently high sperm motility, viability and mitochondria activity, and one boar showed consistently low values. Intra-boar variability was high in the other boars. Screening of SP (n = 12 samples) for protein markers using mass spectrometry identified three protein candidates of which the granulin precursor, legumain and AWN were 0.5 to 0.9 log2-fold less abundant (p < 0.05) in SP-resistant compared to SP-sensitive samples. Lipidome analysis by mass spectrometry revealed 568 lipids showing no difference between the SP-groups. The most abundant lipids were cholesterol (42,442 pmol), followed by phosphatidylserine (20,956 pmol) and ether-linked phosphatidylethanolamine (13,039 pmol). In conclusion, three candidate proteins were identified which might be indicative of SP-tolerance of sperm during long-term storage. Noteworthy, a first lipidomic profile of boar SP is presented.

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Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 98%

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Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Höfner

Luther

Palladini

et al. 2020

IJMS

Self Cite

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“…When insofar routinary semen analysis are hardly capable to identify sub-fertile sires 1 , 2 ; finding effective biomarkers to predict the potential fertility of sires used in artificial insemination (AI) programs is a recurring challenge of the livestock industry; requiring alternative ways. One, and perhaps the most appropriate way to meet this challenge might be to explore the molecular composition of seminal plasma (SP) and of spermatozoa 3 , 4 . Indeed, some SP and sperm proteins have been postulated as suitable biomarkers of fertility 5 – 8 .…”

Section: Introductionmentioning

confidence: 99%

Granulocyte-macrophage colony stimulating factor (GM-CSF) is fully expressed in the genital tract, seminal plasma and spermatozoa of male pigs

Padilla

Martínez-Hernández

Barranco

et al. 2020

Sci Rep

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Granulocyte-macrophage colony stimulating factor (GM-CSF) is a pro-inflammatory cytokine identified in boar seminal plasma (SP) but until now unexplored in terms of place of production and its association to spermatozoa. This study aimed to explore these aspects by evaluating the presence of GM-CSF in porcine reproductive organs (testes, epididymis and accessory sex glands), SP and mature spermatozoa (from cauda epididymis and ejaculated) using Western blot (WB), immunohistochemistry and immunocytochemistry. Positive labelling was obtained in tissues, SP and spermatozoa. In reproductive organs, WB revealed three forms of GM-CSF with different glycosylation degrees (15, 31 and 40 kDa). In SP and epididymal fluid, the GM-CSF appeared only in its active form while in spermatozoa the GM-CSF form present varied among sperm sources. Non-viable spermatozoa showed more GM-CSF than viable spermatozoa (14.87 ± 1.98 RU vs. 7.25 ± 0.52 RU) of fluorescence intensity. In conclusion, GM-CSF is widely present in the reproductive tract of male pigs, attached to the spermatozoa already in the epididymis as well as verted to SP. Consequently, the GM-CSF ought to regulate male genital tract and sperm function as well as mediating initial inflammatory responses and further mediating later immune actions by the female to semen deposition.

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“…Additionally, stallions that are satisfactorily fertile under normal field conditions can produce semen that after freezing and thawing results in very low pregnancy rates [ 10 ]. Several factors influence the cryo-survival of stallion sperm including freezing regimes [ 12 , 50 , 51 ], oxidative and osmotic stress, ice crystal formation, toxicity of the cryoprotectants [ 8 , 52 , 53 ], sample processing [ 54 ] and variability among stallions [ 11 ]. Consequently, the attachment of cryopreserved equine spermatozoa to equine OECs or zona pellucida in vitro is reduced compared to that of fresh spermatozoa [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen–Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status

Gimeno

Bariani

Laiz-Quiroga

et al. 2021

Animals

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Cryopreservation by negatively affecting sperm quality decreases the efficiency of assisted reproduction techniques (ARTs). Thus, we first evaluated sperm motility at different conditions for the manipulation of equine cryopreserved spermatozoa. Higher motility was observed when spermatozoa were incubated for 30 min at 30 × 106/mL compared to lower concentrations (p < 0.05) and when a short centrifugation at 200× g was performed (p < 0.05). Moreover, because sperm suitable for oocyte fertilization is released from oviduct epithelial cells (OECs), in response to the capacitation process, we established an in vitro OEC culture model to select a sperm population with potential fertilizing capacity in this species. We demonstrated E-cadherin and cytokeratin expression in cultures of OECs obtained. When sperm–OEC cocultures were performed, the attached spermatozoa were motile and presented an intact acrosome, suggesting a selection by the oviductal model. When co-cultures were incubated in capacitating conditions a greater number of alive (p < 0.05), capacitated (p < 0.05), with progressive motility (p < 0.05) and with the intact acrosome sperm population was observed (p < 0.05) suggesting that the sperm population released from OECs in vitro presents potential fertilizing capacity. Improvements in handling and selection of cryopreserved sperm would improve efficiencies in ARTs allowing the use of a population of higher-quality sperm.

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The role of seminal plasma in the liquid storage of spermatozoa

Cited by 27 publications

References 61 publications

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Granulocyte-macrophage colony stimulating factor (GM-CSF) is fully expressed in the genital tract, seminal plasma and spermatozoa of male pigs

Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen–Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status

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