The Role of Sodium-Potassium—Activated Adenosine Triphosphatase in the Reabsorption of Sodium by the Kidney*

Katz, Adrian I.; Epstein, Franklin H.

doi:10.1172/jci105689

Cited by 241 publications

(80 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been implicated in the process of active reabsorption of sodium from the glomerular filtrate by renal tubules (Katz & Epstein 1967). In a previous study from our laboratory (Wald et al 1983), evidence was presented to support the concept that the kidney responds to chronic changes in the filtered load of sodium with an adaptive increase in the specific activity of the transport enzyme Na-K-ATPase.…”

Section: Introductionmentioning

confidence: 90%

Renal Na-K-ATPase hyperactivity in diabetic Psammomys obesus is related to glomerular hyperfiltration but is insulin-independent

Scherzer

Nachliel²,

Bar‐On³

et al. 2000

Journal of Endocrinology

View full text Add to dashboard Cite

Psammomys obesus, a desert rodent, develops diabetes when displaced from its natural environment and fed a high energy diet in the laboratory. This study was designed to examine variations in renal function in relation to the diabetic state with emphasis on changes in Na-K-ATPase activity.The following groups of Psammomys were studied: (1) Animals fed a saltbush diet; a low energy/high salt diet (natural). (2) Animals fed a low energy/low salt diet (laboratory). Both 1 and 2 were normoglycemic and normoinsulinemic and thus served as control. (3) Animals fed a high energy diet (group C) who were hyperglycemic and hyperinsulinemic; this group was divided into two subgroups: C1 presented with glomerular hyperfiltration rate and C2 with glomerular hypofiltration rate. (4) Animals fed a high energy diet presenting with hyperglycemia-hypoinsulinemia (group D). (5) Group D+I, similar to group D but treated with external insulin (2 U/24 h).Groups D and C1, whose glomerular filtration rose above normal by 30% and 70% respectively, exhibited metabolic similarity to Type I and Type II diabetes. In these groups, Na-K-ATPase activity in the cortex increased by 80-100% and in the medulla by 180% (P<0·001 vs control). In group C2 with reduced glomerular filtration rate (GFR), Na-K-ATPase activity did not differ from control. In group D+I, with normalized glomerular filtration rate, Na-K-ATPase activity was similar to control. There was a linear and significant correlation between GFR and Na-K-ATPase activity both in the cortex and in the medulla.These experiments present a well defined animal model of diabetes mellitus. Variations in glucose and in insulin did not correlate with Na-K-ATPase activity. These results clearly demonstrated that Na-K-ATPase activity in the diabetic Psammomys was determined by glomerular filtration but was independent of plasma glucose or insulin levels.

show abstract

Section: Introductionmentioning

confidence: 90%

Renal Na-K-ATPase hyperactivity in diabetic Psammomys obesus is related to glomerular hyperfiltration but is insulin-independent

Scherzer

Nachliel²,

Bar‐On³

et al. 2000

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Cell coats of a thickness between 100 and 5000 A, especially in microvilli, have been reported (30). In the present study, a clear indication for the specialization within the cell envelope has been obtained, but no information is available concern- ing at what level of the cell development this occurs The whole enveloping membrane contains MgATPase This may be considered as a hint for a common genotyplc precursor of this membrane This precursor must develop into a membrane of different phenotype, in which tile apical part (microvllli) possesses alkaline phosphatase and the above mentioned coat, whereas the basal part (membranes of the basal infoldings) contains the Na-K-ATPase, a key enzyme for active transport (31), (32). The latter enzyme is being considered as a general marker and constituent of a mammalian plasma membrane Almost nothing is known about the ontogenetic development of this enzyme in kidney.…”

Section: T~e Jovr~a~ Ov C~ll Biology • Volu~e 54 1972mentioning

confidence: 99%

The Polarity of the Proximal Tubule Cell in Rat Kidney

Heidrich¹,

Kinne²,

Kinne-Saffran³

et al. 1972

The Journal of Cell Biology

217

View full text Add to dashboard Cite

Two different membrane fractions were obtained from a brush-border fraction of rat kidney cortex by using their different electrical surface charges in preparative free-flow electrophoresis. One membrane fraction contained only morphologically intact microvilli and was characterized by a high specific activity of alkaline phosphatase. The other fraction morphologically resembled classical plasma membranes by possessing junctional complexes and a high Na-K-ATPase activity The contamination of the isolated membrane fractions by other cell organelles was extremely low These two fractions represent the apical (luminal) and the basal (interstitial) area of the renal proximal tubule cell membrane and clearly demonstrate the polarity of this cell.

show abstract

“…In addition, an increased blood flow may lead to the reestablishments of enzyme sys tem of energy metabolism and supply of substrates, which are responsible for the improve ment of transtubular movement of sodium ions (26)(27)(28)(29)(30). The results obtained in the pre sent study do not allow us to conclude whether cortisol acts directly on the transport mecha nism at the cellular level or indirectly via changes in renal blood flow.…”

Section: Discussionmentioning

confidence: 99%

Effects of Aldosterone and Cortisol on Henle's Loop in the Adrenalectomized Rat's Kidney

Sakai

Murayama

1971

Jpn.J.Pharmacol

View full text Add to dashboard Cite

It is generally accepted that urine is concentrated by the mammalian kidney through the operation of a countercurrent mechanism in the renal medulla and that active sodium transport out of the ascending limb of Henle's loop plays an important role in this mecha nism. In addition, it has been reported that in the adrenalectomized rat urine volume is decreased and the corticomedullary osmotic gradient lowered (1, 2).Experiments studying the effects of aldosterone on free water clearance (3) and the attainment of maximum urinary osmolality (4), have suggested that aldosterone may cause an increased rate of transport of sodium from the inside of the ascending limb of Henle's loop to the surrounding tissue. On the other hand, the action of glucocorticoids, particu larly their effects on the loop of Henle have not yet been precisely defined. Experiments dealing with free water reabsorption (T°H2O) indicated, that glucocorticoids may enhance sodium transport at the ascending limb (5, 6). Hierholzer's experiments (7) suggested that cortisol affects the water permeability of distal tubular segments. In the present experiments we have attempted, by microperfusion of Henle's loop in the kidneys of ad renalectomized rats, to clarify the mode of action of corticosteroids on these tubular seg ments. METHODSMale albino rats weighing 150-230g were adrenalectomized and given free access to 0.9% saline and a dry pellet diet. Intact rats were kept on tap water. The following groups of rats were studied (a) intact animals on controls, (b) adrenalectomized animals 18-24 hours, 4 days and 7-9 days after operation, (c) animals adrenalectomized 7-9 days previously and given 12.5 micrograms of d-aldosterone monoacetate (Aldocorten, Ciba) subcutaneously prior to anesthesia. A further dose of 3.1 micrograms was given intra venously when the kidney had been prepared for micropuncture, (d) animals adrenalecto mized 7-9 days previously and given a subcutaneous injection of 2.5 mg cortisol (alcohol free, Merck) for 2 days. In some animals of this group, 5 mg of cortisol was given daily for 3 days prior to micropuncture. (e) The final group of animals were given both a] dosterone and cortisol similarly to those of group c and d.

show abstract

The Role of Sodium-Potassium—Activated Adenosine Triphosphatase in the Reabsorption of Sodium by the Kidney*

Cited by 241 publications

References 41 publications

Renal Na-K-ATPase hyperactivity in diabetic Psammomys obesus is related to glomerular hyperfiltration but is insulin-independent

Renal Na-K-ATPase hyperactivity in diabetic Psammomys obesus is related to glomerular hyperfiltration but is insulin-independent

The Polarity of the Proximal Tubule Cell in Rat Kidney

Effects of Aldosterone and Cortisol on Henle's Loop in the Adrenalectomized Rat's Kidney

Contact Info

Product

Resources

About