Soil temperature change has considerable impact on land surface energy and water balances, and hence on changes in weather/climate, surface/subsurface hydrology, and ecosystems. However, little is known regarding the spatiotemporal variations and influencing factors of changes in hourly soil temperature (depth: 5–320 cm) in low-latitude highland areas. This study analyzed the hourly soil temperature at each hour during 2004–2020 and at 08:00, 14:00, and 20:00 (Beijing Time) during 1961–2020. The results revealed the following. 1) As soil depth increased, average soil temperature increased in autumn and winter, and decreased annually and in spring and summer. It exhibited significant increase during 00:00–23:00 annually, seasonally, and monthly, especially at depths of 40–320 cm during 2004–2020. Average soil temperature increased at 08:00 and decreased at 14:00 and 20:00 with increasing soil depth, but the opposite trend was found annually, seasonally, and monthly at 08:00, 14:00, and 20:00 during 1961–2020. 2) With increasing elevation, average soil temperature decreased at 08:00, 14:00, and 20:00 at depths of 5–20 cm, and showed significant increase trend at 08:00 and 14:00 at depths of 10–20 cm (except at 14:00 at 10-cm depth). 3) At 5-cm depth, the critical accumulated soil temperature of ≥12°C and 14°C extended the potential growing season during 1961–2020. 5) Significant uptrend of hourly soil temperature annually, seasonally, and monthly potentially leads to additional release of carbon to the atmosphere and increased soil respiration, reinforcing climate warming. These findings contribute to better understanding of the variation of shallow soil temperatures and land–atmosphere interactions in low-latitude highland areas.