The role of specific immunoglobulins in antibody-dependent cell-mediated cytotoxicity assays during Babesia bovis infection

Goff, Will L.; Wagner, G. G.; Craig, Thomas M.; Long, R.F.

doi:10.1016/0304-4017(84)90118-3

Cited by 14 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The kinetics of parasite clearance suggested that recognition of infected erythrocytes (IRBCs) 3 by serum Abs was important for removal of the parasites from the circulation (4). Ab-dependent cellular cytotoxicity (ADCC) (5,6), and opsonization and phagocytosis (7), are two mechanisms by which cells of the immune system may eliminate IRBCs. However, serum Abs could also effect the destruction of B. bovis IRBCs by disruption of cytoadherence and IRBC sequestration.…”

mentioning

confidence: 99%

Selection of Babesia bovis-Infected Erythrocytes for Adhesion to Endothelial Cells Coselects for Altered Variant Erythrocyte Surface Antigen Isoforms

O’Connor

Allred

2000

The Journal of Immunology

View full text Add to dashboard Cite

Sequestration of Babesia bovis-infected erythrocytes (IRBCs) in the host microvasculature is thought to constitute an important mechanism of immune evasion. Since Ig is considered to be important for protection from disease, an in vitro assay of B. bovis sequestration was used to explore the ability of anti-B. bovis Ig to interfere with IRBC cytoadhesion, and to identify IRBC surface Ags acting as endothelial cell receptors. Bovine infection sera reactive with the IRBC surface inhibited and even reversed the binding of IRBCs to bovine brain capillary endothelial cells (BBECs). This activity is at least partially attributable to serum IgG. IgG isolated from inhibitory serum captured the variant erythrocyte surface ag 1 (VESA1) in surface-specific immunoprecipitations of B. bovis-IRBCs. Selection for the cytoadhesive phenotype concurrently selected for antigenic and structural changes in the VESA1 Ag. In addition, the anti-VESA1 mAb, 4D9.1G1, proved capable of effectively inhibiting and reversing binding of adhesive, mAb-reactive parasites to BBECs, and by immunoelectron microscopy localized VESA1 to the external tips of the IRBC membrane knobs. These data are consistent with a link between antigenic variation and cytoadherence in B. bovis and suggest that the VESA1 Ag acts as an endothelial cell ligand on the B. bovis-IRBC.

show abstract

mentioning

confidence: 99%

Selection of Babesia bovis-Infected Erythrocytes for Adhesion to Endothelial Cells Coselects for Altered Variant Erythrocyte Surface Antigen Isoforms

O’Connor

Allred

2000

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…The importance of IFN‐γ as a key immunomodulator for both humoral and cellular immune mechanisms against Plasmodium and Babesia has been building for several years. 11‐15 Evidence indicates that specific antibody functions as an opsonin to enhance cellular mechanisms, such as antibody‐dependent cellular cytotoxicity 16‐18 and phagocytosis of infected erythrocytes and/or merozoites. 19‐22 The importance of IgG 2 as an opsonin is well known, and it is now known that IFN‐γ plays a central role in the switch to this isotype.…”

mentioning

confidence: 99%

Babesia bovis Immunity: In Vitro and in Vivo Evidence for IL‐10 Regulation of IFN‐γ and iNOS^a

Goff¹,

Johnson²,

Cluff³

1998

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

IL-10 has been shown to have profound immunoregulatory attributes and in the bovine appears to downregulate both Th1- and Th2-like responses. Using RT-PCR, we demonstrate IL-10 in vitro down-regulation of mRNA expression of iNOS, the cytokines involved in nitric oxide signal transduction initiation (IFN-gamma and TNF-alpha), and other mononuclear phagocyte associate cytokines. In addition, using RT-PCR with peripheral blood leukocytes and spleen leukocytes, the Griess reaction, and a killing assay, we provide evidence for the importance of iNOS in a successful immune response to B. bovis infection and for high and persistent IL-10 mRNA expression when the immune response is unsuccessful. We also provide evidence that antibody developed early after an initial infection appears to lack protective attributes (neutralizing and opsonic). Together, the data suggests that IL-10 and IFN-gamma are critical molecules involved in the response to this intraerythrocytic protozoan infection.

show abstract

“…Effector arms of the adaptive immune system include antigen-specific antibodies, which have been hypothesized to target both infected erythrocytes and extracellular merozoites. There is also evidence that antibody-dependent cell-mediated cytotoxicity (ADCC) plays a role in parasite control during acute infection [31], [32]. A recombinant version of the Bd37 protein has been shown to stimulate antibody-directed protective immunity to B. divergens when administered in the context of an adjuvant [33], [34].…”

Section: Resultsmentioning

confidence: 99%

Targeting Cattle-Borne Zoonoses and Cattle Pathogens Using a Novel Trypanosomatid-Based Delivery System

et al. 2011

View full text Add to dashboard Cite

Trypanosomatid parasites are notorious for the human diseases they cause throughout Africa and South America. However, non-pathogenic trypanosomatids are also found worldwide, infecting a wide range of hosts. One example is Trypanosoma (Megatrypanum) theileri, a ubiquitous protozoan commensal of bovids, which is distributed globally. Exploiting knowledge of pathogenic trypanosomatids, we have developed Trypanosoma theileri as a novel vehicle to deliver vaccine antigens and other proteins to cattle. Conditions for the growth and transfection of T. theileri have been optimised and expressed heterologous proteins targeted for secretion or specific localisation at the cell interior or surface using trafficking signals from Trypanosoma brucei. In cattle, the engineered vehicle could establish in the context of a pre-existing natural T. theileri population, was maintained long-term and generated specific immune responses to an expressed Babesia antigen at protective levels. Building on several decades of basic research into trypanosomatid pathogens, Trypanosoma theileri offers significant potential to target multiple infections, including major cattle-borne zoonoses such as Escherichia coli, Salmonella spp., Brucella abortus and Mycobacterium spp. It also has the potential to deliver therapeutics to cattle, including the lytic factor that protects humans from cattle trypanosomiasis. This could alleviate poverty by protecting indigenous African cattle from African trypanosomiasis.

show abstract

The role of specific immunoglobulins in antibody-dependent cell-mediated cytotoxicity assays during Babesia bovis infection

Cited by 14 publications

References 6 publications

Selection of Babesia bovis-Infected Erythrocytes for Adhesion to Endothelial Cells Coselects for Altered Variant Erythrocyte Surface Antigen Isoforms

Selection of Babesia bovis-Infected Erythrocytes for Adhesion to Endothelial Cells Coselects for Altered Variant Erythrocyte Surface Antigen Isoforms

Babesia bovis Immunity: In Vitro and in Vivo Evidence for IL‐10 Regulation of IFN‐γ and iNOS^a

Targeting Cattle-Borne Zoonoses and Cattle Pathogens Using a Novel Trypanosomatid-Based Delivery System

Contact Info

Product

Resources

About

The role of specific immunoglobulins in antibody-dependent cell-mediated cytotoxicity assays during Babesia bovis infection

Cited by 14 publications

References 6 publications

Selection of Babesia bovis-Infected Erythrocytes for Adhesion to Endothelial Cells Coselects for Altered Variant Erythrocyte Surface Antigen Isoforms

Selection of Babesia bovis-Infected Erythrocytes for Adhesion to Endothelial Cells Coselects for Altered Variant Erythrocyte Surface Antigen Isoforms

Babesia bovis Immunity: In Vitro and in Vivo Evidence for IL‐10 Regulation of IFN‐γ and iNOSa

Targeting Cattle-Borne Zoonoses and Cattle Pathogens Using a Novel Trypanosomatid-Based Delivery System

Contact Info

Product

Resources

About

Babesia bovis Immunity: In Vitro and in Vivo Evidence for IL‐10 Regulation of IFN‐γ and iNOS^a