The Role of Sperm Membrane Potential and Ion Channels in Regulating Sperm Function

Pinto, Francisco M.; Odriozola, Ainize; Candenas, Luz; Subirán, Nerea

doi:10.3390/ijms24086995

Cited by 7 publications

(5 citation statements)

References 201 publications

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“…The activity of ENaC is controlled by intracellular pH, Ca 2+ , Cl − , phosphorylation or amiloride. Previous findings support the involvement of this channel in sperm movement and regulation of sperm resting potential, which becomes more negative during capacitation-associated hyperpolarization [33][34][35].…”

Section: Sodium Channelssupporting

confidence: 67%

Signaling Roleplay between Ion Channels during Mammalian Sperm Capacitation

Benko,

Urminská,

Ďuračka

et al. 2023

Biomedicines

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In order to accomplish their primary goal, mammalian spermatozoa must undergo a series of physiological, biochemical, and functional changes crucial for the acquisition of fertilization ability. Spermatozoa are highly polarized cells, which must swiftly respond to ionic changes on their passage through the female reproductive tract, and which are necessary for male gametes to acquire their functional competence. This review summarizes the current knowledge about specific ion channels and transporters located in the mammalian sperm plasma membrane, which are intricately involved in the initiation of changes within the ionic milieu of the sperm cell, leading to variations in the sperm membrane potential, membrane depolarization and hyperpolarization, changes in sperm motility and capacitation to further lead to the acrosome reaction and sperm–egg fusion. We also discuss the functionality of selected ion channels in male reproductive health and/or disease since these may become promising targets for clinical management of infertility in the future.

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Section: Sodium Channelssupporting

confidence: 67%

Signaling Roleplay between Ion Channels during Mammalian Sperm Capacitation

Benko,

Urminská,

Ďuračka

et al. 2023

Biomedicines

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“…Ca concentrations in the prostate, seminal vesicles, and epididymis may be associated with sperm infertility [22] . Na/K-ATPase plays an essential role in maintaining membrane potential (Em) and electrochemical gradients (Na + and K + ) across the sperm membrane, which is related to the structure and ultrastructure of the spermatozoa membrane [23] .…”

Section: Resultsmentioning

confidence: 99%

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Baharun,

Rahmi,

Handarini

et al. 2024

J Adv Vet Anim Res

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Objective: To determine the correlation between the molecular weight (MW) of proteins in seminal plasma and spermatozoa and the quality of fresh and frozen semen production in Pasundan bulls. Materials and methods: Nine selected Pasundan bulls, aged 5–10 years, from the Regional Artificial Insemination Center at Ciamis, West Java, Indonesia, were used in the study, with fresh semen sperm motility ≥70% and <70%. We analyzed the motility, viability, integrity of the intact plasma membrane (IPM), and the morphological characteristics of spermatozoa. 1D-SDS-PAGE analysis was performed to determine the protein profile by assessing MW, depicted as bands on the gel. Results: The motility, viability, and IPM of spermatozoa had lower values (p < 0.05) in Pasundan bulls named Bagaskara and Kertarajasa compared to the other bulls. Proteins with MW 35–50 kDa were not detected in the seminal plasma of Pasundan bulls, exhibiting low quality in fresh semen. The correlation analysis showed that the non-detected proteins with MW 35–50 kDa in seminal plasma correlated with spermatozoa motility (r = 0.421), viability (r = 0.424), and IPM (r = 0.428) so that fresh semen quality was low in both Pasundan bulls. Analysis of semen volume, spermatozoa concentration, and spermatozoa motility showed that the average frozen semen production of Pasundan bulls per ejaculate was 128.73 ± 15.35 straws. Conclusion: Protein analysis based on MW is a predictive indicator for the quality of fresh semen and the production of frozen semen in Pasundan bulls. Evaluation parameters of fresh semen quality by MW analysis can be used to select Pasundan bulls in Indonesia.

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“…To outline the pathways of soot-mediated blocking of the acrosome reaction, we will discuss a few important biochemical and physiological alterations that the spermatozoa experience in their travel throughout the female reproductive tract [ 34 , 35 ]. When the gametes are away from the egg, they are highly negatively charged and the capacitation and acrosome reaction are impeded by the cholesterol and Zn 2+ ions in the seminal plasma [ 35 ] or via molecules of sialoglycoproteins, sialic acid, steroid sulfates and sulfated carbohydrates attached to the sperm body [ 34 ]. When approaching the oocyte, the spermatozoa undergo maturation, associated with increased permeability of the plasma membrane to calcium, stimulating the acrosome reaction.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Acrosomal Status of Human Spermatozoa Subjected to Hydrophobic Carbon Soot Treatments

Esmeryan,

Rangelov,

Chaushev

2024

Nanomaterials

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The fourth industrial revolution extensively reshapes the reality we are living in by blurring the boundaries of physical, digital and biological worlds. A good example is the previously unthinkable incursion of nanoscale waste materials, such as soot, into the technologies for assisted reproduction. Although the rapeseed oil soot may efficiently enhance the progressive motility of human spermatozoa, it is yet unknown whether this material induces undesirable oxidative stress and premature acrosome reaction, endangering the sperm-oocyte fusion and blastocyst formation. In an attempt to clarify this issue, we reveal that the three-hour incubation of human semen mixed with three main types of soot does not cause oxidative stress and spontaneous acrosome reaction of the sperm. These unique findings are attributed to synchronous elimination and stabilization of the oxidants via hydrogen bonding to the acidic groups of the soot (i.e., C=O and/or C-O-C) and electron donation by its basic chemical sites (i.e., C-OH and/or COOH). Moreover, the soot nanoparticles are electrostatically attracted by discrete positively charged areas on the sperm head, increasing its negative charge and in some cases interfering the acrosome reaction. Such novel mechanistic insights emphasize the credibility of rapeseed oil soot to confidently shift from the purely diagnostic and therapeutic phases in reproductive medicine to research dealing with the effect of carbon nanomaterials on the embryo development and implantation.

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The Role of Sperm Membrane Potential and Ion Channels in Regulating Sperm Function

Cited by 7 publications

References 201 publications

Signaling Roleplay between Ion Channels during Mammalian Sperm Capacitation

Signaling Roleplay between Ion Channels during Mammalian Sperm Capacitation

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Oxidative Stress and Acrosomal Status of Human Spermatozoa Subjected to Hydrophobic Carbon Soot Treatments

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