The role of stallion seminal proteins in fertilisation

Töpfer‐Petersen, Edda; Ekhlasi-Hundrieser, Mahnaz; Kirchhoff, Christiane; Leeb, Tosso; Sieme, Harald

doi:10.1016/j.anireprosci.2005.06.018

Cited by 110 publications

(80 citation statements)

References 59 publications

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“…For example, equine cysteine-rich secretory protein (CRISP) genes were found to be significantly associated with male fertility (Schambony et al 1998). The CRISP proteins represent the major equine fraction of seminal plasma proteins and were found to be involved in sperm-oocyte fusion (Töpfer-Petersen et al 2005). Furthermore, Giesecke et al (2011) chose three genes, namely angiotensin-converting enzyme (ACE), sperm autoantigenic protein 17 (SPA17) and FSH beta subunit (FSHB), to test as candidates for determining Hanoverian stallion fertility, finding that haplotypes of all three genes significantly contributed to the paternal and embryonic fertility components of the pregnancy rate per oestrus.…”

Section: Discussionmentioning

confidence: 99%

Implication of transcriptome profiling of spermatozoa for stallion fertility

Suliman

Becker

Wimmers

2018

Reprod. Fertil. Dev.

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Abstract. Poor fertility of breeding stallions is a recognised problem in the equine industry. The aim of the present study was to detect molecular pathways using two groups of stallions that differed in pregnancy rates as well as in the proportion of normal and motile spermatozoa. RNA was isolated from spermatozoa of each stallion and microarray data were analysed to obtain a list of genes for which transcript abundance differed between the groups (P #0.05, fold change $1.2). In all, there were 437 differentially expressed (DE) genes between the two groups (P # 0.05, fold change $1.2). Next, the DE genes were analysed using Database for Annotation, Visualisation, and Integrated Discovery (DAVID). Finally, ingenuity pathways analysis (IPA) was used to identify top biological functions and significant canonical pathways associated with the DE genes. Analysis using the DAVID database showed significant enrichment in the gene ontology (GO) term 'RNA binding' (P ¼ 0.05) and in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cytokinecytokine receptor interaction (P ¼ 0.02). Furthermore, IPA analysis showed interconnected biological functions and canonical pathways involved in the regulation of spermatogenesis and male fertility. In addition, significantly enriched metabolic pathways were identified. In conclusion, the present study has identified, for the first time, molecular processes in stallion spermatozoa that could be associated with stallion fertility.

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Section: Discussionmentioning

confidence: 99%

Implication of transcriptome profiling of spermatozoa for stallion fertility

Suliman

Becker

Wimmers

2018

Reprod. Fertil. Dev.

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“…Moreover, some SP-proteins, once into the female genital tract after natural mating or AI, modulate the uterine immune response against the foreign semen, facilitating sperm transport [11], sperm-oocyte fusion [12] and the development of a healthy embryo [13].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the porcine seminal plasma proteome comparing ejaculate portions

Pérez-Patiño

Barranco

Parrilla

et al. 2016

Journal of Proteomics

102

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Full identification of SP-proteins remains challenging, particularly in some livestock species such as porcine. This experimental study aims to provide an extensive proteomic analysis of boar SP and to generate a public accessible database of boar SP-proteome. A SP-pool from 33 entire ejaculates from 11 boars (3 ejaculates per boar) was analyzed to characterize the boar SP-proteome. Moreover, 20 ejaculates collected in fractions (P1: first 10 mL of sperm rich ejaculate fraction (SRF), P2: rest of SRF and P3: post-SRF) from 5 boars (4 ejaculates per boar) were analyzed to evaluate differentially expressed SP-proteins among portions. SP-samples were subjected to a combination of SEC, 1-D SDS PAGE and NanoLC-ESI-MS/MS followed by functional bioinformatics analysis.The identified proteins were quantified from normalized LFQ intensity data. A total of 33,557 spectra corresponding to 8,189 peptides and 536 SP-proteins were identified with ≥ 95% Confidence (Unused Score > 1.3) and a false discovery rate (FDR) ≤ 1%. Of the 536 SP-proteins, 409 were identified in Sus Scrofa taxonomy and 374 of them were Biological Significance: This proteomic study provides the major characterization of the boar SP-proteome with more than 250 proteins first reported. The boar SP-proteome is described so that a spectral library can be built for relative 'label free' protein quantitation with SWATH approach. This proteomic profiling allows the creation of a publicly accessible database of the boar SP-proteome, as a first step for further understanding the role of SP-proteins in reproductive outcomes as well as for identification of biomarkers for sperm quality and fertility.

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“…O plasma seminal proporciona boas condições para a manutenção da motilidade, da sobrevivência e do transporte espermático, tanto no sistema reprodutor do macho, quanto da fêmea (TÖPFER-PETERSEN et al, 2004). Entretanto, estudos sobre as proteínas do plasma seminal e da membrana espermática ainda precisam definir os tipos de proteínas e os mecanismos de ação que afetam a viabilidade desses gametas (ASADPOUR et al, 2007).…”

Section: Introductionunclassified

“…O plasma seminal dos Bovídeos possui a família das proteínas designadas de BSP-A1/-A2 e BSP-A3 (15-17kDa), (MANJUNATH, 1984), e FMP, relacionadas à motilidade progressiva (37,5kDa). Em eqüinos, as proteínas HSP-1 (22-25kDa), HSP-2 (25kDa, pI 6,5-6,9), HSP-7 (14kDa) e HSP-12 kDa pertencem à família das espermadesinas, e a SP-1 possui correlação positiva com a fertilidade (TÖPFER-PETERSEN et al, 2004).…”

Section: Introductionunclassified