The role of stroma in the expansion of odontogenic cysts and adenomatoid odontogenic tumor: A polarized microscopy study

Mahajan, Aarti; Mahajan, Mahendra C; Ganvir, SM; Hazarey, Vinay K.

doi:10.4103/0976-9668.116988

Cited by 6 publications

(8 citation statements)

References 20 publications

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“…En este caso, los resultados fueron presentados en relación al colágeno tipo I, con características de fibras densas y gruesas, cuya birrefringencia es intensa entre los colores amarillo y rojo (Junqueira et al, 1978;Vij et al, 2011). Nuestros hallazgos coinciden con los estudios de Mahajan et al (2013) y Vij et al, que señalan el predominio de estas tonalidades en las paredes de los quistes radiculares, principalmente el color anaranjado.…”

Section: Discussionunclassified

Periodontitis Apical: Caracterización Histológica y Morfométrica de Quistes Radiculares y Granulomas Periapicales

et al. 2018

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RESUMEN: La periodontitis apical es una patología inflamatoria que afecta los tejidos periapicales de un diente desvitalizado. El objetivo de este estudio fue caracterizar histológica y morfométricamente las lesiones de quistes y granulomas utilizando microscopía óptica. Se analizaron seis biopsias obtenidas de dientes con indicación de exodoncia. El análisis histológico se realizó mediante microscopía óptica y microfotografías, con análisis de contraste de imágenes y conteo celular mediante ImageJ. Descripción de las características histológicas: en los quistes se observaron cavidades rodeadas de epitelio escamoso estratificado no queratinizado y una cápsula fibrosa compuesta de fibrocitos, fibroblastos e infiltrado inflamatorio crónico; en los granulomas se observaron capilares, una capsula fibrosa de fibrocitos/fibroblastos y un infiltrado inflamatorio de predominio linfocitario. Cuantificación del número celular de infiltrado inflamatorio: para quistes fue de 9,2 cel/10000 µm 2 , mientras que para granulomas fue de 20,8 cel/10000 µm 2 , sin diferencias estadísticas significativas entre ambos (p=0,654). Cuantificación del número celular de fibrocitos/fibroblastos: para quistes fue de 15,4 cel/10000 µm 2 , mientras que para granulomas fue de 18,5 cel/10000 µm 2 , sin diferencia estadística significativa (p=0,499). Porcentaje de colágeno tipo I: para los quistes fue de 37,8±19,2 %, mientras que para granulomas fue de 33,8±23,3 %, sin diferencias estadísticas significativas (p=0,704). Se observó una correlación negativa moderada para el infiltrado inflamatorio (R=0,637) y una correlación positiva baja para fibrocitos/fibroblastos (R=0,121), en relación a la cantidad de colágeno tipo I. Medición del área de las lesiones periapicales: el promedio total de las lesiones fue de 10,7±5,0 mm 2 , siendo el mayor tamaño un quiste de 18,1 mm 2 y el menor un granuloma de 5,2 mm 2. El análisis histológico permite realizar un diagnóstico diferencial de lesiones con características similares y así definir el tratamiento más adecuado.

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Section: Discussionunclassified

Periodontitis Apical: Caracterización Histológica y Morfométrica de Quistes Radiculares y Granulomas Periapicales

et al. 2018

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“…Whereas the less prevalence of greenish yellow fibers in DC might indicate that collagen fiber bundles are more closely packed and are perhaps not under the influence of intense collagenolytic activity in contrast to OKC. 8 Our study suggests that OKC is more aggressive lesion than DC by means of identifying abnormally packed collagen fibers in it. Thus the nature of collagen fibers as studied by picrosirius red polarization method may be useful diagnostic tool to differentiate between these cysts and may also help to predict their nature in term of biologic behavior and prognosis.…”

Section: 11mentioning

confidence: 93%

“…Hence, Picrosirius-Polarization method is an exceptionally simple, specific, sensitive, consistent and cost effective method for observing collagen fibers. 7,8 Experiments by Vedtofte et al in nude mice showed that the behavior of OKC depends on both the epithelium as well as underlying stroma. 9 In DC, connective tissue behaves like a reactive tissue and initiates an expansile growth.…”

Section: Discussionmentioning

confidence: 99%

Role of Thickness of Collagen Fibers in Odontogenic Keratocyst and Dentigerous Cyst– A Picrosirius Red Polarized Microscopic Study

Jahagirdar¹

2017

JMSCR

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“…Structures other than collagen does not show any birefringence when stained with picrosirius red. 3,[12][13][14] The principle behind the picrosirius red staining of the collagen fibers is that picrosirius red is an acidic dye, which binds to the basic amino acids of the collagen (ionic bonding). Few researchers also state that interaction between the picrosirius red and collagen can also be unionic.…”

Section: Discussionmentioning

confidence: 99%

“…1 Jaws host wide variety of cysts and tumors encompassing the spectrum of lesions ranging from developmental cysts to malignant neoplasms. 2,3 Few of these cysts and tumors exhibit a biologically aggressive course resulting in destruction of adjacent and deeper tissues. 2 Many researchers have emphasized on the role of epithelium in the biological behavior of these lesions, however, connective tissue especially collagen, which being understudied, may also have significant participation in its biological behavior.…”

Section: Introductionmentioning

confidence: 99%

Collagen and Its Role in predicting the Biological Behavior of Odontogenic Lesions

Kulkarni

Kumari

Jahagirdar

et al. 2017

The Journal of Contemporary Dental Practice

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Introduction: Odontogenic cysts and tumors have variable recurrence rates. Recurrence rate is mainly due to the activity of the epithelium. The epithelium of these lesions has been investigated extensively in regard to their role in proliferative and aggressive behavior of the lesions. However, the role of the connective tissue wall in their behavior has not been studied as extensively. Collagen is an essential part of the connective tissue as a whole and fibrous wall of cystic lesions especially. It is demonstrated by picrosirius red dye staining combined with polarization microscopy. This method permits the evaluation of the nature of the collagen fibers in addition to their thickness. Materials and methods:A total of 56 histopathologically diagnosed cases comprising odontogenic follicle, dentigerous cyst, unicystic ameloblastoma, keratocystic odontogenic tumor (KCOT), multicystic/solid ameloblastoma, and ameloblastic carcinoma were taken and stained using picrosirius red stain and evaluated using a polarizing microscope.Results: Collagen fibers in odontogenic follicles and dentigerous cysts showed predominant orange-red birefringence; fibers in unicystic ameloblastoma and KCOT showed both orange red and greenish-yellow birefringence; and fibers of multicystic/ solid ameloblastoma showed predominant greenish-yellow birefringence and ameloblastic carcinoma that showed almost complete greenish birefringence. As the biological behavior of the lesions in the spectrum studied progress toward aggressive nature, increase in immature collagen fibers is noticed. Conclusion:This study suggests that the nature of collagen fibers plays a pivotal role in predicting the biological behavior of odontogenic lesions. Clinical significance: Aggressive nature of the odontogenic lesions is determined by both the epithelium and the connective tissue components (collagen). Studying the nature and type of collagen helps in predicting its biological behavior. Collagen and Its Role in predicting the

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The role of stroma in the expansion of odontogenic cysts and adenomatoid odontogenic tumor: A polarized microscopy study

Cited by 6 publications

References 20 publications

Periodontitis Apical: Caracterización Histológica y Morfométrica de Quistes Radiculares y Granulomas Periapicales

Periodontitis Apical: Caracterización Histológica y Morfométrica de Quistes Radiculares y Granulomas Periapicales

Role of Thickness of Collagen Fibers in Odontogenic Keratocyst and Dentigerous Cyst– A Picrosirius Red Polarized Microscopic Study

Collagen and Its Role in predicting the Biological Behavior of Odontogenic Lesions

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