The Role of Sucrose in the Development of Oral Biofilm in a Simulated Mouth System

Ismail, Wan Izhan Nawawi Wan; Razak, Fathilah Abdul; Rahim, Zubaidah Hj Abdul

doi:10.3844/ojbsci.2006.62.66

Cited by 4 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It may be the role of sucrose in the TSB medium to grow the bacteria in our experiment. The initial stage of plaque formation can be sufficiently achieved by saliva, and oral streptococcus can grow continuously in human whole saliva at the expense of various organic components present in the saliva (glycoproteins) that support their growth [20]. Furthermore, sucrose can be utilized by oral streptococcus to produce extracellular polysaccharides in dental biofilms.…”

Section: Resultsmentioning

confidence: 99%

“…Sucrose consumption can lead to a decrease in pH in both nonstarved and starved biofilms of S. mutans [21]. To ingest sucrose, S. mutans produces three types of Gtf that convert sucrose into glucan: (i) Gtf that synthesizes water-soluble glucan, (ii) Gtf that synthesizes water-insoluble glucan, and (iii) Gtf that synthesizes both types of glucan [2,5,20].…”

Section: Resultsmentioning

confidence: 99%

“…Consequently, sucrose plays a role in the development of late but not early settler oral biofilms [20].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Propolis on Streptococcus mutans Biofilm Formation

Djais¹,

Putri²,

Jemmy³

et al. 2019

Pesqui. bras. odontopediatria clín. integr.

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Propolis on Streptococcus mutans Biofilm Formation

Djais¹,

Putri²,

Jemmy³

et al. 2019

Pesqui. bras. odontopediatria clín. integr.

View full text Add to dashboard Cite

Natural antimicrobials subtilosin and lauramide arginine ethyl ester synergize with conventional antibiotics clindamycin and metronidazole against biofilms of Gardnerella vaginalis but not against biofilms of healthy vaginal lactobacilli

Algburi

Volski

Chikindas

2015

Pathogens and Disease

View full text Add to dashboard Cite

The purpose of this study was to evaluate the ability of clindamycin and metronidazole to synergize with natural antimicrobials against biofilms of bacterial vaginosis (BV)-associated Gardnerella vaginalis. Minimum bactericidal concentrations for biofilm cells (MBCs-B) were determined for each antimicrobial. The MBCs-B of lauramide arginine ethyl ester (LAE), subtilosin, clindamycin and metronidazole were 50, 69.5, 20 and 500 μg mL(-1), respectively. A checkerboard assay and isobologram were used to analyze the type of interactions between these antimicrobials. The combination of metronidazole with natural antimicrobials did not inhibit planktonic lactobacilli. Clindamycin with either LAE or with subtilosin was inhibitory for planktonic but not for biofilm-associated lactobacilli. All tested antimicrobial combinations were inhibitory for BV-associated Mobiluncus curtisii and Peptostreptococcus anaerobius. LAE and subtilosin synergized with clindamycin and metronidazole against biofilms of G. vaginalis but not biofilm-associated vaginal lactobacilli. The biofilms of BV-associated pathogens can be controlled by synergistically acting combinations of conventional antibiotics and natural antimicrobials which will help better management of current antibiotics, especially considering robust bacterial resistance. Our findings create a foundation for a new strategy in the effective control of vaginal infections.

show abstract

Exploring the Inhibitory Potential of M. pendans Compounds Against N‐Acetylglucosamine (Mur) Receptor: In Silico Insights Into Antibacterial Activity and Drug‐Likeness

Gartika,

Tumilaar,

Dharsono

et al. 2024

The Scientific World Journal

View full text Add to dashboard Cite

Oral diseases are often caused by bacterial infections, making the inhibition of receptors like N‐acetylglucosamine critical in preventing bacterial formation. The plant Myrmecodia pendans (M. pendans) is known for its diverse bioactivities and may serve as a promising source for developing new antibacterial agents. This study employs in silico methods to predict the inhibitory mechanisms, pharmacokinetics, and drug‐likeness of compounds isolated from M. pendans. Three compounds were evaluated for their inhibitory effects on the MurA and MurB receptors using the AutoDock4 molecular docking software, with visualizations performed using the BIOVIA Discovery Studio Visualizer. The binding affinities obtained for compounds 1, 2, and 3 to the MurA receptor were −9.42, −9.57, and −6.84 kcal/mol, respectively, while their binding affinities to the MurB receptor were −11.25, −10.55, and −8.69 kcal/mol. These affinities were found to be stronger than those of fosfomycin (benchmark compound) but weaker than the native ligands of the respective receptors. Key amino acid residues involved in the binding to MurA were identified as Cys115 and Asp305, while Ser82 and Asn83 were noted for MurB. In the ADMET prediction and drug‐likeness analysis, some compounds met the necessary criteria, whereas others did not. Although all the three compounds demonstrated strong predicted inhibitory activity against MurA and MurB receptors, the analysis suggests that Compound 2 may hold the most promise as a potential antibacterial agent, warranting further investigation.

show abstract

The Role of Sucrose in the Development of Oral Biofilm in a Simulated Mouth System

Cited by 4 publications

References 34 publications

Effect of Propolis on Streptococcus mutans Biofilm Formation

Effect of Propolis on Streptococcus mutans Biofilm Formation

Natural antimicrobials subtilosin and lauramide arginine ethyl ester synergize with conventional antibiotics clindamycin and metronidazole against biofilms of Gardnerella vaginalis but not against biofilms of healthy vaginal lactobacilli

Exploring the Inhibitory Potential of M. pendans Compounds Against N‐Acetylglucosamine (Mur) Receptor: In Silico Insights Into Antibacterial Activity and Drug‐Likeness

Contact Info

Product

Resources

About