The Role of SWI/SNF Chromatin Remodeling Complexes in Hormone Crosstalk

Sarnowska, Elżbieta; Gratkowska, Dominika M.; Sacharowski, Sebastian; Cwiek, Pawel; Tohge, Takayuki; Fernie, Alisdair R.; Siedlecki, Janusz A.; Koncz, Csaba; Sarnowski, Tadeusz

doi:10.1016/j.tplants.2016.01.017

Cited by 101 publications

(101 citation statements)

References 109 publications

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“…The SWI/SNF complex is involved in steroid hormone action and directly interacts with various steroid hormone receptors including androgen receptor (Sarnowska et al 2016). The androgen receptor pathway was found as one of the most deregulated, with wide spectrum of AR transcript level in ACC patient samples.…”

Section: Discussionmentioning

confidence: 99%

Advanced adenoid cystic carcinoma (ACC) is featured by SWI/SNF chromatin remodeling complex aberrations

Jagielska

Sarnowska

Rusetska

et al. 2018

J Cancer Res Clin Oncol

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PurposeAdenoid cystic carcinoma (ACC) is a rare neurotropic cancer with slow progression occurring in salivary glands and less frequently in other body parts. ACC is featured by hyperchromatic nuclei and various mutations in genes encoding chromatin-related machineries. The ACC treatment is mainly limited to the radical surgery and radiotherapy while the chemotherapy remains ineffective. As the knowledge about molecular basis of ACC development is limited, we investigated here the molecular features of this disease.Patients and methodsThis study included 50 patients with ACC. Transcript profiling of available ACC samples vs normal salivary gland tissue, quantitative real-time PCR (qRT-PCR) transcript level measurements and the immunohistochemistry (IHC) for SWI/SNF chromatin remodeling complex (CRC) subunits and androgen receptor on surgery-derived paraffin-embedded samples were performed.ResultsTranscriptomic study followed by Gene Ontology classification indicated alteration of chromatin-related processes, including downregulated transcript levels of main SWI/SNF CRC subunits and elevated expression of BRM ATPase-coding SMARCA2 gene in ACC. Subsequent IHC indicated broad accumulation of BRM ATPase and several SWI/SNF subunits, suggesting affected control of their protein level in ACC. The IHC revealed ectopic, heterogeneous expression of androgen receptor (AR) in some ACC cells.ConclusionsOur study indicated that ACC features aberrant expression of genes controlling chromatin status and structure. We found that the balance between SWI/SNF classes is moved towards the BRM ATPase-containing complex in ACC. As BRM is known to be involved in chemoresistance in cancer cells, this observation may be the likely explanation for ACC chemoresistance.Electronic supplementary materialThe online version of this article (10.1007/s00432-018-2783-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Advanced adenoid cystic carcinoma (ACC) is featured by SWI/SNF chromatin remodeling complex aberrations

Jagielska

Sarnowska

Rusetska

et al. 2018

J Cancer Res Clin Oncol

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“…In root, the ZmCHB101-regulated genes were stratified into plant hormone signal transduction process, including different receptors, phosphatases and transcriptional regulators in auxin, cytokinin, ABA, ethylene, jasmonic acid and salicylic acid signaling pathways. Previously, it was reported that A. thaliana SWI/SNF complex regulates different hormone signaling pathways and their crosstalk in A. thaliana 40. It is thus possible that CRCs-mediated transcriptional regulation is a conserved feature between A. thaliana and maize.…”

Section: Discussionmentioning

confidence: 99%

“…Consistent with these previous findings, we found here that nucleosome occupancy of putative promoter region of two marker genes, GRMZM2G047065 and AC208201_FG002 , was significantly decrease in the two independent ZmCHB101 RNAi lines, R101 and RS1, compared with WT, leading to their upregulated expression. Several SWI/SNF subunits in A. thaliana have also been shown to interact with different signaling and transcriptional machineries40. It is therefore possible that ZmCHB101 acts as the co-activator or suppressor of these components to modulate the expression of its target genes during both normal growth/development and under stress conditions.…”

Section: Discussionmentioning

confidence: 99%

The Core Subunit of A Chromatin-Remodeling Complex, ZmCHB101, Plays Essential Roles in Maize Growth and Development

Jiang

et al. 2016

Sci Rep

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ATP-dependent chromatin remodeling complexes play essential roles in the regulation of diverse biological processes by formulating a DNA template that is accessible to the general transcription apparatus. Although the function of chromatin remodelers in plant development has been studied in A. thaliana, how it affects growth and development of major crops (e.g., maize) remains uninvestigated. Combining genetic, genomic and bioinformatic analyses, we show here that the maize core subunit of chromatin remodeling complex, ZmCHB101, plays essential roles in growth and development of maize at both vegetative and reproductive stages. Independent ZmCHB101 RNA interference plant lines displayed abaxially curling leaf phenotype due to increase of bulliform cell numbers, and showed impaired development of tassel and cob. RNA-seq-based transcriptome profiling revealed that ZmCHB101 dictated transcriptional reprogramming of a significant set of genes involved in plant development, photosynthesis, metabolic regulation, stress response and gene expressional regulation. Intriguingly, we found that ZmCHB101 was required for maintaining normal nucleosome density and 45 S rDNA compaction. Our findings suggest that the SWI3 protein, ZmCHB101, plays pivotal roles in maize normal growth and development via regulation of chromatin structure.

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“…ATP-dependent chromatin remodelers (e.g., SWI/SNF complex) were found to influence chromatin structure and its transcriptional activity by modulating nucleosome re-positioning and the overall nuclear organization [55]. Evolutionarily conserved multi-protein machinery (ATP-dependent chromatin re-modelling complexes) control DNA accessibility, chromatin structure, enable histone variant replacement and alter histone-DNA interaction during stress response [63,64].…”

Section: Epigenetic Regulation Of Chromatin Structurementioning

confidence: 99%

Epigenomics of Plant Responses to Environmental Stress

Kumar

2018

Epigenomes

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Genome-wide epigenetic changes in plants are being reported during development and environmental stresses, which are often correlated with gene expression at the transcriptional level. The sum total of the biochemical changes in nuclear DNA, post-translational modifications in histone proteins and variations in the biogenesis of non-coding RNAs in a cell is known as an epigenome. These changes are often responsible for variation in the expression of the gene without any change in the underlying nucleotide sequence. The changes might also cause variation in chromatin structure resulting in the changes in function/activity of the genome. The epigenomic changes are dynamic with respect to the endogenous and/or environmental stimuli, which affect phenotypic plasticity of the organism. Both the epigenetic changes and variation in gene expression might return to the pre-stress state soon after the withdrawal of the stress. However, a part of the epigenetic changes may be retained, which is reported to play a role in acclimatization and adaptation as well as in the evolutionary process. Probable exploitation of epigenome-engineering for improved stress tolerance in plants has become essential for better utilization of the genetic resources. This review delineates the importance of epigenomics towards the possible improvement of plant responses to environmental stresses for climate resilient agriculture.

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The Role of SWI/SNF Chromatin Remodeling Complexes in Hormone Crosstalk

Cited by 101 publications

References 109 publications

Advanced adenoid cystic carcinoma (ACC) is featured by SWI/SNF chromatin remodeling complex aberrations

Advanced adenoid cystic carcinoma (ACC) is featured by SWI/SNF chromatin remodeling complex aberrations

The Core Subunit of A Chromatin-Remodeling Complex, ZmCHB101, Plays Essential Roles in Maize Growth and Development

Epigenomics of Plant Responses to Environmental Stress

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