The Role of Synthetic Extracellular Matrices in Endothelial Progenitor Cell Homing for Treatment of Vascular Disease

Williams, Priscilla A.; Silva, Eduardo A.

doi:10.1007/s10439-015-1400-x

Cited by 20 publications

(16 citation statements)

References 92 publications

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“…Recently, considerable attention has been paid to the important role of MMPs play in cartilage degeneration, which starts from ECM metabolism imbalance involving proteoglycan degradation and collagen damage [48, 49]. Degradation of the ECM is also involved in the incipient stage of atherosclerosis and occurs over the course of the progressive disease [50, 51]. Therefore, MMP1 may have a subtle regulatory effect on the pathogenesis of atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction

et al. 2017

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Transient ischemic attack (TIA) is a predictor for cerebral infarction (CI), and early diagnosis of TIA is extremely important for the prevention of CI. We set out to identify novel antibody biomarkers for TIA and CI, and detected matrix metalloproteinase 1 (MMP1), chromobox homolog 1 (CBX1), and chromobox homolog 5 (CBX5) as candidate antigens using serological identification of antigens by recombinant cDNA expression cloning (SEREX) and Western blotting to confirm the presence of serum antibodies against the antigens. Amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) revealed that serum antibody levels were significantly higher in patients with TIA or acute-phase CI (aCI) compared with healthy donors (P < 0.01). Spearman’s correlation analysis and multivariate logistic regression analysis demonstrated that levels of anti-MMP1, anti-CBX1, and anti-CBX5 antibodies were associated with age, cigarette-smoking habits, and blood pressure. Thus, serum levels of antibodies against MMP1, CBX1, and CBX5 could potentially serve as useful tools for diagnosing TIA and predicting the onset of aCI.

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Section: Discussionmentioning

confidence: 99%

Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction

et al. 2017

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“…Evidence suggests that the injured endothelial monolayer may be regenerated by circulating bone marrow-derived endothelial progenitor cells (EPC), which accelerate re-endothelialization and protect against the initiation and progression of atherosclerosis [203–205,206]. EPC represent less than 1% of the cells in blood.…”

Section: Endothelial Progenitor Cellsmentioning

confidence: 99%

“…It should be noted, however, that controversy exists with respect to the identification and characterization of ‘EPC’ which may actually encompass several different populations, as well as with regard to their definitive role in the pathophysiology of atherosclerosis vs in angiogenesis [208,209]. Insufficient blood flow resulting in local tissue ischemia is recognized as a potent stimulus for recruitment of EPC [206]; EPC express chemokine receptor type 4 (CXCR-4) receptors that bind stromal-derived factor-1 (SDF-1) that is released at sites of ischemia [210]. Higher circulating levels of progenitor cells reflect greater repair capacity and have been shown to reduce the progression of atherosclerosis [211 ].…”

Section: Endothelial Progenitor Cellsmentioning

confidence: 99%

Vascular endothelium – Gatekeeper of vessel health

2016

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The vascular endothelium is an interface between the blood stream and the vessel wall. Changes in this single cell layer of the artery wall are believed of primary importance in the pathogenesis of vascular disease/atherosclerosis. The endothelium responds to humoral, neural and especially hemodynamic stimuli and regulates platelet function, inflammatory responses, vascular smooth muscle cell growth and migration, in addition to modulating vascular tone by synthesizing and releasing vasoactive substances. Compromised endothelial function contributes to the pathogenesis of cardiovascular disease; endothelial 'dysfunction' is associated with risk factors, correlates with disease progression, and predicts cardiovascular events. Therapies for atherosclerosis have been developed, therefore, that are directed towards improving endothelial function.

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“…[25] Using synthetic ECM as an EPC delivery vehicle promotes cell survival, engraftment efficiency, endothelial differentiation, and tissue repair. [26] A recent study revealed that ECM proteins enhance the capture of late-outgrowth EPCs under shear flow. [27] These findings indicate that the developed BBV augments the survival and differentiation of transplanted EPCs and atorvastatin represents the synergetic effect on neovascularization in ischemic tissues.…”

Section: Treatment Of Ischemic Disease In Mouse Modelsmentioning

confidence: 99%

Tissue Engineered Bio‐Blood‐Vessels Constructed Using a Tissue‐Specific Bioink and 3D Coaxial Cell Printing Technique: A Novel Therapy for Ischemic Disease

Gao

Lee

Jang

et al. 2017

Adv Funct Materials

272

188

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Endothelial progenitor cells (EPCs) are a promising cell source for the treatment of several ischemic diseases for their potentials in neovascularization. However, the application of EPCs in cell-based therapy has shown low therapeutic efficacy due to hostile tissue conditions after ischemia. In this study, a bio-blood-vessel (BBV) is developed, which is produced using a novel hybrid bioink (a mixture of vascular-tissue-derived decellularized extracellular matrix (VdECM) and alginate) and a versatile 3D coaxial cell printing method for delivering EPC and proangiogenic drugs (atorvastatin) to the ischemic injury sites. The hybrid bioink not only provides a favorable environment to promote the proliferation, differentiation, and neovascularization of EPCs but also enables a direct fabrication of tubular BBV. By controlling the printing parameters, the printing method allows to construct BBVs in desired dimensions, carrying both EPCs and atorvastatin-loaded poly(lactic-co-glycolic) acid microspheres. The therapeutic efficacy of cell/drug-laden BBVs is evaluated in an ischemia model at nude mouse hind limb, which exhibits enhanced survival and differentiation of EPCs, increased rate of neovascularization, and remarkable salvage of ischemic limbs. These outcomes suggest that the 3D-printed ECM-mediated cell/drug implantation can be a new therapeutic approach for the treatment of various ischemic diseases.

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The Role of Synthetic Extracellular Matrices in Endothelial Progenitor Cell Homing for Treatment of Vascular Disease

Cited by 20 publications

References 92 publications

Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction

Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction

Vascular endothelium – Gatekeeper of vessel health

Tissue Engineered Bio‐Blood‐Vessels Constructed Using a Tissue‐Specific Bioink and 3D Coaxial Cell Printing Technique: A Novel Therapy for Ischemic Disease

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