2011
DOI: 10.2478/s11756-011-0075-5
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The role of TerW protein in the tellurite resistance of uropathogenic Escherichia coli

Abstract: Abstract:The ter operon, which is found on a large conjugative plasmid, pTE53, from the uropathogenic strain Escherichia coli KL53, mediates heavy metal ion resistance to tellurium compounds. Here we present the function of the terW gene, which is transcribed from its own promoter in the opposite direction to the terZABCDE loci and encodes a protein of 155 amino acids. A TerW protein containing an N-terminal His-tag was overexpressed, purified and analysed for the in vitro DNA-binding. Green fluorescent protei… Show more

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Cited by 4 publications
(5 citation statements)
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“…Potassium tellurite is highly toxic to most microorganisms and can inhibit the growth of most bacteria, which can save a lot of labor work in the epidemiological study of fecal samples. Other pathogenic bacteria that are clinically resistant to tellurite, such as Bacillus anthracis , Yersinia, and enterohemorrhagic E. coli O157:H7, etc., ( 14 , 16 , 42 , 43 ) were not identified in this study. These bacteria, as well as KP, can reduce the tellurite (TeO ) of soluble tellurite to the less toxic Te element and deposit intracellularly to form black colonies ( 44 ).…”
Section: Discussionmentioning
confidence: 82%
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“…Potassium tellurite is highly toxic to most microorganisms and can inhibit the growth of most bacteria, which can save a lot of labor work in the epidemiological study of fecal samples. Other pathogenic bacteria that are clinically resistant to tellurite, such as Bacillus anthracis , Yersinia, and enterohemorrhagic E. coli O157:H7, etc., ( 14 , 16 , 42 , 43 ) were not identified in this study. These bacteria, as well as KP, can reduce the tellurite (TeO ) of soluble tellurite to the less toxic Te element and deposit intracellularly to form black colonies ( 44 ).…”
Section: Discussionmentioning
confidence: 82%
“…TerW protein is the first known functional ter gene product and binds specifically to the promoter region of the ter operon. TerW protein controls tellurite resistance levels by inducing overexpression of the E. coli ter gene ( 14 ). At present, there are many studies on the tellurite resistance of E .…”
Section: Discussionmentioning
confidence: 99%
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“…At least one PhoP binding site is located in the regulatory region between terZ and OA04_37930 , one more is present upstream of the terW gene. terW (6.9x induced) is located a few genes away and was shown to be responsible for transcriptional control of ter genes in E. coli ( Valkovicova et al, 2011 ). The part of the ter operon regulatory region most conserved between E. coli and Pectobacterium sp.…”
Section: Resultsmentioning
confidence: 99%
“…sequences contains a 25 bp palindrome ( Supplementary Figure 4 and Supplementary File 3 ). Its position corresponds to the region protected by TerW ( Valkovicova et al, 2011 ) and probably corresponds to the TerW binding site. Three putative TerW binding sites could be located within the ter cluster in Pve : two in the middle of the intergenic region between the divergently transcribed operons (terZABCDE and OA04_37930-OA04_37890 ) and the third one is in front of terY1 .…”
Section: Resultsmentioning
confidence: 99%