The Role of Testosterone Hormone on Crystal Formation Through the Renal Expression of Osteopontin in Male Wistar Rat

Abstract

This study aims to investigate the role of testosterone hormone on crystal formation through osteopontin expression. This was an experimental study with post-test only control design. A total of 12 rats aged 10 weeks were divided into two groups which are male rats group fed with water containing EG (Ethylene Glycol) 0.75% and male rats group fed with water containing EG (Ethylene Glycol) 0.75% and were injected with testosterone hormone. Furthermore, the rats were observed and fed with standard diet for 30 days. Then, both groups were terminated. The kidneys were extracted and examined for Osteopontin expression. Histopathology examination was also performed in both groups The result of the study showed that the mean kidney osteopontin expression in male rats fed with water containing EG 0.75% and received testosterone hormone injection were significantly higher (29.16 ± 9.072) compared to male rat groups fed with water containing EG 0.75% alone (11.42 ± 3.692) with p value 0.001. Histopathology examination with HE stain revealed that both groups demonstrated the presence of stone in kidney tubules, kidney swelling and pelvic ectasis. This feature suggested the presentation of hydronephrosis as a result of stone obstruction. As a conclusion, this study shed light on the role of hormonal sex of testosterone involving in crystal formation through osteopontin expression. Keywords: urolithiasis, testosterone, sex hormone, osteopontin