The Role of the Active Oxygen Produced from Gp91phox NADPH Oxidase on the Newborn Weight of Mouse Pups

Gp91phox NADPH oxidase modulates litter size by regulating mucin1 in the uterus of mice

“…We previously showed that the birth weights of offspring were lower in gp91phox −/mice than in C57BL/ 6j mice [Hiramoto et al 2016b]. We proposed the following mechanism for this reduction in body weight: ROS derived from gp91phox activates inflammatory NLRP3, which increases the expression of caspase-1; caspase-1 in turn increases the expression of IL-1β, and IL-1β is then believed to increase the expression of IGF-1, which subsequently increases birth weight.…”

“…ROS are known to be associated with ovulation [Shkolnik et al 2011] and are involved in the ovarian follicle rhexis. In gp91phox −/mice, in which little gp91phox-dependent ROS are generated, decreased ovulation occurs, subsequently reducing the number of births [Hiramoto et al 2016b]. However, the observed reduction in the number of follicles in gp91phox −/mice does not appear to influence fertilization [Brannstrom et al 1995].…”

“…Our results showed that IL-1β played an important role in the observed decrease in weight. Gp91phox −/mice exhibit reduced birth weights of offspring compared with that of C57BL/6j mice [Hiramoto et al 2016b]. Thus, we evaluated whether IL-1β may be related to the number of births by administration of an IL-1β inhibitor.…”

“…Gp91phox is a subunit of the enzymatic complex and binds with p22phox [Sumimoto 2008]. We previously measured the number of births and weights of offspring in gp91phox-knockout (gp91phox −/-) mice and found that these mice tended to have smaller litters than control mice [Hiramoto et al 2016b]. In addition, silencing of the gp91phox gene suppressed the expression of insulin-like growth factor-1 (IGF-1) through a ROS-dependent mechanism, resulting in low birth weight offspring.…”

ACTH/cAMP-Mediated Skin Pigmentation Caused by 5-Fluorouracil Administration

“…The solvent-injected animals were administered 0.08% DMSO. 19) Preparation and staining of the skin around the buttocks. We obtained samples from the skin around the buttocks after 8 weeks of starting the experiment.…”