The role of the bifunctional enzyme, fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase, in carbon partitioning during salt stress and salt tolerance in Rice (Oryza sativa L.)

Udomchalothorn, Thanikarn; Maneeprasobsuk, Somporn; Bangyeekhun, Eakaphan; Boon-Long, Preeda; Chadchawan, Supachitra

doi:10.1016/j.plantsci.2008.11.009

Cited by 21 publications

(19 citation statements)

References 36 publications

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“…Although 23 genes of LPT123‐TC171 seemed to be lacking in function, LPT123‐TC171 retained normal growth and development. Moreover, this plant showed better adaptation under a salt‐stress condition, as previously reported (Udomchalothorn et al, 2009). Therefore, the deleterious mutation found in this genome did not negatively affect the main metabolic processes of the cell, but rather responded to stress adaption mechanisms in rice.…”

Section: Discussionsupporting

confidence: 84%

“…The point mutations found in the exome analysis may result in the drought‐ and salt‐stress response change in the LPT123‐TC171 mutant line, as many of them were shown to be responsive to salt and drought stresses and involved in regulation of gene expression and signal transduction. Interestingly, we did not detect the mutations in fructose‐6‐phosphate‐2‐kinase/fructose‐2,6‐bisphosphatase (Udomchalothorn et al, 2009) and OsNUC1 (Sripinyowanich et al, 2013) genes. Presumably, the changes in gene expression of these two genes found previously were caused by mutation in the regulatory regions rather than in the exon.…”

Section: Discussionmentioning

confidence: 63%

“…LPT123‐TC171 is a ST and DT variant rice line that was generated from somaclonal variegation of the callus of LPT123 rice ( Oryza sativa ‘Leung Pratew 123’) that is more susceptible to abiotic stress (Thikart et al, 2005; Udomchalothorn et al, 2009; Pongprayoon et al, 2013). Some of the adaptive mechanisms of LPT123‐TC171 to salt stress have been reported (Udomchalothorn et al, 2009; Sripinyowanich et al, 2013), where the gene expression level of at least two genes, fructose‐6‐phosphate‐2‐kinase/fructose‐2,6‐bisphosphatase (Udomchalothorn et al, 2009) and OsNUC1 (Sripinyowanich et al, 2013), were found to be different between the LPT123 and LPT123‐TC171 genotypes, and this may contribute to the ST ability in the LPT123‐TC171 line.…”

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confidence: 99%

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Molecular Karyotyping and Exome Analysis of Salt‐Tolerant Rice Mutant from Somaclonal Variation

et al. 2014

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LPT123-TC171 is a salt-tolerant (ST) and drought-tolerant (DT) rice line that was selected from somaclonal variation of the original Leuang Pratew 123 (LPT123) rice cultivar. The objective of this study was to identify the changes in the rice genome that possibly lead to ST and/or DT characteristics. The genomes of LPT123 and LPT123-TC171 were comparatively studied at the four levels of whole chromosomes (chromosome structure including telomeres, transposable elements, and DNA sequence changes) by using next-generation sequencing analysis. Compared with LPT123, the LPT123-TC171 line displayed no changes in the ploidy level, but had a significant deficiency of chromosome ends (telomeres). The functional genome analysis revealed new aspects of the genome response to the in vitro cultivation condition, where exome sequencing revealed the molecular spectrum and pattern of changes in the somaclonal variant compared with the parental LPT123 cultivar. Mutation detection was performed, and the degree of mutations was evaluated to estimate the impact of mutagenesis on the protein functions. Mutations within the known genes responding to both drought and salt stress were detected in 493 positions, while mutations within the genes responding to only salt stress were found in 100 positions. The possible functions of the mutated genes contributing to salt or drought tolerance were discussed. It was concluded that the ST and DT characteristics in the somaclonal variegated line resulted from the base changes in the salt-and drought-responsive genes rather than the changes in chromosome structure or the large duplication or deletion in the specific region of the genome.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 63%

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confidence: 99%

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Molecular Karyotyping and Exome Analysis of Salt‐Tolerant Rice Mutant from Somaclonal Variation

et al. 2014

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“…Fructose-bisphosphate aldolase (FBPase), starch synthase and isoflavone reductase are closely related to the sucrose synthesis and photosynthetic products formation [42][43][44]. In our study, S decreased the abundance of FBPase (B4FR47, A0A096RD67), starch synthase (B8A2L4) and isoflavone reductase (B4FD74) (Fig.…”

Section: Effects Of Light Intensity On Photosynthesis-related Proteinsmentioning

confidence: 59%

Physiological and comparative proteomic analysis provides new insights into the effects of shade stress in maize (Zea mays L.)

et al. 2020

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Background: Shade stress, a universal abiotic stress, suppresses plant growth and production seriously. However, little is known regarding the protein regulatory networks under shade stress. To better characterize the proteomic changes of maize leaves under shade stress, 60% shade (S) and supplementary lighting (L) on cloudy daylight from tasseling stage to physiological maturity stage were designed, the ambient sunlight treatment was used as control (CK). Isobaric tag for relative and absolute quantification (iTRAQ) technology was used to determine the proteome profiles in leaves. Results: Shading significantly decreased the SPAD value, net photosynthetic rate, and grain yield. During two experimental years, grain yields of S were reduced by 48 and 47%, and L increased by 6 and 11%, compared to CK. In total, 3958 proteins were identified by iTRAQ, and 2745 proteins were quantified including 349 proteins showed at least 1.2-fold changes in expression levels between treatments and CK. The differentially expressed proteins were classified into photosynthesis, stress defense, energy production, signal transduction, and protein and amino acid metabolism using the Web Gene Ontology Annotation Plot online tool. In addition, these proteins showed significant enrichment of the chloroplasts (58%) and cytosol (21%) for subcellular localization. Conclusions: 60% shade induced the expression of proteins involved in photosynthetic electron transport chain (especially light-harvesting complex) and stress/defense/detoxification. However, the proteins related to calvin cycle, starch and sucrose metabolisms, glycolysis, TCA cycle, and ribosome and protein synthesis were dramatically depressed. Together, our results might help to provide a valuable resource for protein function analysis and also clarify the proteomic and physiological mechanism of maize underlying shade stress.

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“…In contrast, fructose bisphosphatase (FBPase-1) is an enzyme that converts fructose-1,6-bisphosphate to fructose 6-phosphate in gluconeogenesis [37]. The synthesis and degradation of fructose-2, 6-biophosphate are catalyzed by the bifunctional enzyme 6-phosphofructo-2-kinase/fructose bisphosphatase 2 (PFK-2/ FBPase-2) [38]. The up-accumulated abundance of the ATP-PFK and FBPase-2, and down-accumulated abundance of FBPase-1 may have resulted in the accumulation of Fru-1, 6-P2 to produce more energy through glycolysis to resist or adapt to chilling stress (Table 1).…”

Section: Daps Involved In Carbohydrate and Energy Metabolismmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways responding to chilling stress in maize seedlings

Wang

Shan

et al. 2016

Journal of Proteomics

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The role of the bifunctional enzyme, fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase, in carbon partitioning during salt stress and salt tolerance in Rice (Oryza sativa L.)

Cited by 21 publications

References 36 publications

Molecular Karyotyping and Exome Analysis of Salt‐Tolerant Rice Mutant from Somaclonal Variation

Molecular Karyotyping and Exome Analysis of Salt‐Tolerant Rice Mutant from Somaclonal Variation

Physiological and comparative proteomic analysis provides new insights into the effects of shade stress in maize (Zea mays L.)

iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways responding to chilling stress in maize seedlings

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